Anti-CGRP antibody [4901] is a mouse monoclonal antibody that is used to detect CGRP in Flow cytometry, ICC/IF, IHC-Fr. Suitable for Guinea pig, Human, Mouse, Rat samples.
- Recognizes Calcitonin Gene-Related Peptide (CGRP), Rat beta CGRP, and Human alpha CGRP (with 10-fold lower sensitivity compared to Rat alpha CGRP)
- Cited in over 150 publications
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (AB81887), expandable thumbnail](https://content.abcam.com/products/images/cgrp-antibody-4901-ab81887--immunocytochemistry-immunofluorescence-img14692.gif "Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (AB81887)")
Publications
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- The journal of headache and pain 24:1542023Progesterone distribution in the trigeminal system and its role to modulate sensory neurotransmission: influence of sex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAida Maddahi,Karin Warfvinge,Anja Holm,Jacob C A Edvinsson,Philip Victor Reducha,Spyridoula Kazantzi,Kristian A Haanes,Lars EdvinssonPubMed 37957603
- International journal of molecular sciences 24:2023Switching Rat Resident Macrophages from M1 to M2 Phenotype by Iba1 Silencing Has Analgesic Effects in SNL-Induced Neuropathic Pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoxana-Olimpia Gheorghe,Andreea Violeta Grosu,Melania Magercu,Mihail-Sebastian Ghenghea,Cristina Elena Zbarcea,Alexandra Tanase,Simona Negres,Alexandru Filippi,Gabriela Chiritoiu,Mihaela Gherghiceanu,Sorina Dinescu,Gisela Gaina,Damir Sapunar,Violeta RistoiuPubMed 37958812
- The journal of headache and pain 24:1412023The potent analgesia of intrathecal 2R, 6R-HNK via TRPA1 inhibition in LF-PENS-induced chronic primary pain model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAn-Ran Liu,Zhen-Jia Lin,Ming Wei,Yuan Tang,Hui Zhang,Xiang-Ge Peng,Ying Li,Yu-Fan Zheng,Zhi Tan,Li-Jun Zhou,Xia FengPubMed 37858040
- The journal of headache and pain 24:1372023GFAP-NpHR mediated optogenetic inhibition of trigeminal nucleus caudalis attenuates hypersensitive behaviors and thalamic discharge attributed to infraorbital nerve constriction injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElina Kc,Jaisan Islam,Hyong Kyu Kim,Young Seok ParkPubMed 37821818
- Biomedicines 11:2023Thoracic Dorsal Root Ganglion Application of Resiniferatoxin Reduces Myocardial Ischemia-Induced Ventricular Arrhythmias.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomoki Yamaguchi,Siamak Salavatian,Yuki Kuwabara,Abigail Hellman,Bradley K Taylor,Kimberly Howard-Quijano,Aman MahajanPubMed 37893094
- International journal of molecular sciences 24:2023Effects of Early Exposure to Isoflurane on Susceptibility to Chronic Pain Are Mediated by Increased Neural Activity Due to Actions of the Mammalian Target of the Rapamycin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQun Li,Reilley Paige Mathena,Fengying Li,Xinzhong Dong,Yun Guan,Cyrus David MintzPubMed 37762067
- iScience 26:1077922023Spinal TAOK2 contributes to neuropathic pain via cGAS-STING activation in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Zhang,Ang Li,Yu-Fan Liu,Zhong-Ming Sun,Bing-Xin Jin,Jia-Piao Lin,Yan Yang,Yong-Xing YaoPubMed 37720090
- British journal of pharmacology 180:3194-32142023Inhibition of Brd4 alleviates osteoarthritis pain via suppression of neuroinflammation and activation of Nrf2-mediated antioxidant signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Sun,Xing-He Wang,Fan-He Song,Dan-Yang Li,Shao-Jie Gao,Long-Qing Zhang,Jia-Yi Wu,Dai-Qiang Liu,Li-Wei Wang,Ya-Qun Zhou,Wei MeiPubMed 37485568
- iScience 26:1076902023Human iPS cell-derived sensory neurons can be infected by SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnthony Flamier,Punam Bisht,Alexsia Richards,Danielle L Tomasello,Rudolf JaenischPubMed 37680484
- Cells 12:2023Calcitonin Related Polypeptide Alpha Mediates Oral Cancer Pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNguyen Huu Tu,Kenji Inoue,Parker K Lewis,Ammar Khan,Jun Hyeong Hwang,Varun Chokshi,Branka Brukner Dabovic,Shanmugapriya Selvaraj,Aditi Bhattacharya,Zinaida Dubeykovskaya,Nathalie M Pinkerton,Nigel W Bunnett,Cynthia A Loomis,Donna G Albertson,Brian L SchmidtPubMed 37443709
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P01256
Reactivity data
Select an application| WB | Flow Cyt | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Human | Not recommended | Expected | Tested | Expected |
| Mouse | Not recommended | Expected | Expected | Predicted |
| Rat | Not recommended | Expected | Expected | Predicted |
| Guinea pig | Not recommended | Expected | Expected | Predicted |
| Mammals | Not recommended | Predicted | Predicted | Predicted |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse, Guinea pig, Mammals | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
Species Mouse | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
Species Guinea pig | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals, Rat, Mouse, Guinea pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
CGRP1/CALCA is a peptide hormone that induces vasodilation mediated by the CALCRL-RAMP1 receptor complex. Dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP. CGRP1 can also bind and activate CALCR-RAMP1 (AMYR1) receptor complex.
Alternative names
Calc, Calca, Calcitonin gene-related peptide 1, CGRP1, Alpha-type CGRP, Calcitonin gene-related peptide I, CGRP-I
Recommended products
Anti-CGRP antibody [4901] is a mouse monoclonal antibody that is used to detect CGRP in Flow cytometry, ICC/IF, IHC-Fr. Suitable for Guinea pig, Human, Mouse, Rat samples.
- Recognizes Calcitonin Gene-Related Peptide (CGRP), Rat beta CGRP, and Human alpha CGRP (with 10-fold lower sensitivity compared to Rat alpha CGRP)
- Cited in over 150 publications
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P01256
- Clone number
- 4901
- Purification technique
- Affinity purification Protein G
- Specificity
ab81887 is specific for Calcitonin Gene Related Peptide. It also recognizes rat beta CGRP and human alpha CGRP (10 fold less sensitivity compared to rat alpha CGRP).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CGRP antibody [4901] (ab81887) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-Fr in human samples.
Anti-CGRP antibody [4901] (ab81887) has been cited over 153 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-CGRP antibody [4901] (ab81887) has high sensitivity and specificity.
Anti-CGRP antibody [4901] (ab81887) has 8 independent reviews from customers.
Anti-CGRP antibody [4901] (ab81887) specifically detects CGRP (UniProt ID: P01256; Molecular weight: 4kDa) and is sold in 100 uL selling sizes.
CGRP (Calcitonin Gene-Related Peptide) is a significant neuropeptide extensively studied in neuroscience. Its role is particularly noted in various neurodegenerative conditions, including Alzheimer's disease (AD). CGRP is involved in modulating inflammation and neuroprotection, making it a valuable marker for assessing neuroinflammatory responses. In AD, changes in CGRP expression can provide insights into the disease's progression and the associated inflammatory processes. This highlights CGRP's importance in understanding and potentially targeting neuroinflammation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CGRP or Calcitonin Gene-Related Peptide is a 37-amino acid neuropeptide with a molecular mass of approximately 3.8 kDa. This peptide exists mainly in two forms alpha-CGRP and beta-CGRP differing slightly in amino acid sequence. CGRP expresses in peripheral and central nervous systems with high levels in the dorsal root ganglia and thyroid gland. It also has a significant presence in the enteric nervous system and some segments of the cardiovascular system. Scientists note that CGRP acts as a potent vasodilator and plays a significant role in transducing pain signals.
- Biological function summary
CGRP contributes to numerous physiological processes including blood flow regulation body temperature control pain transmission and neurogenic inflammation. It acts independently and does not substantially form part of larger protein complexes in its primary activities. Through binding to the CGRP receptor a complex made up of the calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1) CGRP initiates downstream signaling that results in its various biological effects.
- Pathways
Studies place CGRP within the neuropeptide signaling pathway and the vasodilation pathway. It significantly influences the cyclic AMP (cAMP) signaling pathway where it works collaboratively with other neuropeptides like substance P. The interaction with CLR and RAMP1 triggers the production of cAMP which enhances vasodilation and pain transmission processes in the body.
- Associated diseases and disorders
CGRP holds a notable connection to migraines and cardiovascular diseases. Elevated CGRP levels are observed in migraine sufferers and targeted treatments utilizing anti-CGRP antibodies have demonstrated efficacy in reducing migraine frequency. Additionally research links CGRP to blood pressure regulation abnormalities implicating its involvement in hypertension. Both conditions involve complex interactions with other proteins such as substance P and endothelial nitric oxide synthase (eNOS) which modulate vascular tone and inflammation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)
Immunofluorescent staining of CGRP (red) in cryopreserved DRG neurons grown on coverslips using ab81887 at 1/50 dilution. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5.
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