AB189786

Anti-CGRP antibody

Name: Anti-CGRP antibody (ab189786) | Abcam
Brand: Abcam Limited
SKU: AB189786
Price: 535 USD
Availability: InStock

(1 Review)

(9 Publications)

Rabbit Polyclonal CGRP-I antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 9 publications.

View Alternative Names

CALC1, CALCA, Calcitonin gene-related peptide 1, CGRP1, Alpha-type CGRP, Calcitonin gene-related peptide I, CGRP-I

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody (AB189786)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody (AB189786)

Immunofluorescence analysis of Human U2OS cells using Anti-CGRP antibody (ab189786) at dilution of 1 : 100. Blue : DAPI for nuclear staining.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody (AB189786)

Immunofluorescence analysis of Rat C6 cells using Anti-CGRP antibody at dilution of 1 : 100. Blue : DAPI for nuclear staining.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody (AB189786)

Immunofluorescence analysis of Mouse L929 cells using Anti-CGRP antibody at dilution of 1 : 100. Blue : DAPI for nuclear staining.

Supplier Data

Western blot - Anti-CGRP antibody (AB189786)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-CGRP antibody (ab189786) at 1/1000 dilution

All lanes:

Mouse brain at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB

applications

style

left-column

Alternative Products

Primary Antibodies

AB36001

Anti-CGRP antibody

ELISA - Anti-CGRP antibody (AB36001)

Primary Antibodies

AB47856

Biotin Anti-CGRP antibody

Primary Antibodies

AB195387

Anti-CGRP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (AB195387)

Primary Antibodies

AB47027

Anti-CGRP antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (AB47027)

Primary Antibodies

AB81887

Anti-CGRP antibody [4901]

Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (AB81887)

Primary Antibodies

AB135271

Anti-CGRP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (AB135271)

Complementary Products

Assay Kits

AB221828

Human Procalcitonin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Procalcitonin ELISA Kit (AB221828)

Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB108986

Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker

Western blot - Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (AB108986)

Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CGRP or Calcitonin Gene-Related Peptide is a 37-amino acid neuropeptide with a molecular mass of approximately 3.8 kDa. This peptide exists mainly in two forms alpha-CGRP and beta-CGRP differing slightly in amino acid sequence. CGRP expresses in peripheral and central nervous systems with high levels in the dorsal root ganglia and thyroid gland. It also has a significant presence in the enteric nervous system and some segments of the cardiovascular system. Scientists note that CGRP acts as a potent vasodilator and plays a significant role in transducing pain signals.

Biological function summary

CGRP contributes to numerous physiological processes including blood flow regulation body temperature control pain transmission and neurogenic inflammation. It acts independently and does not substantially form part of larger protein complexes in its primary activities. Through binding to the CGRP receptor a complex made up of the calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1) CGRP initiates downstream signaling that results in its various biological effects.

Pathways

Studies place CGRP within the neuropeptide signaling pathway and the vasodilation pathway. It significantly influences the cyclic AMP (cAMP) signaling pathway where it works collaboratively with other neuropeptides like substance P. The interaction with CLR and RAMP1 triggers the production of cAMP which enhances vasodilation and pain transmission processes in the body.

CGRP holds a notable connection to migraines and cardiovascular diseases. Elevated CGRP levels are observed in migraine sufferers and targeted treatments utilizing anti-CGRP antibodies have demonstrated efficacy in reducing migraine frequency. Additionally research links CGRP to blood pressure regulation abnormalities implicating its involvement in hypertension. Both conditions involve complex interactions with other proteins such as substance P and endothelial nitric oxide synthase (eNOS) which modulate vascular tone and inflammation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

CGRP1/CALCA is a peptide hormone that induces vasodilation mediated by the CALCRL-RAMP1 receptor complex (PubMed : 1318039, PubMed : 33602864, PubMed : 9620797). Dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role (PubMed : 3492492). It also elevates platelet cAMP (PubMed : 1318039). CGRP1 can also bind and activate CALCR-RAMP1 (AMYR1) receptor complex (PubMed : 38603770).

See full target information CALCA

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The journal of headache and pain 26:197 PubMed41039196

2025

Inhibition of the METTL3/mA/miR-34a-5p axis suppresses trigeminovascular activation in nitroglycerin-induced migraine via the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Minming Shao,Feng Zhang,Caiyan He,Jiabi Li,Shengdong He

Biomaterials research 28:0118 PubMed39717477

2024

Custom-Made Ce-Mn Bimetallic Nanozyme for the Treatment of Intervertebral Disc Degeneration by Inhibiting Oxidative Stress and Modulating Macrophage M1/M2 Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Wu,Zhenhao Chen,Han Huang,Hongwei Wang,Xianghe Wang,Zian Lu,Haocheng Xu,Xiaosheng Ma,Feng Zeng,Hongli Wang

Cell biology international 48:1755-1765 PubMed39129231

2024

METTL3 mediated ferroptosis in chondrocytes and promoted pain in KOA via HMGB1 m6A modification.

Applications

Unspecified application

Species

Unspecified reactive species

Tianchi Bao,Taiyang Liao,Xuefeng Cai,Binjie Lu,Gaole Dai,Shuai Pei,Yunqing Zhang,Yuwei Li,Bo Xu

Evidence-based complementary and alternative medicine : eCAM 2022:8659462 PubMed36337584

2022

Electroacupuncture Modulates 5-HT-Mediated cAMP/PKA Signaling to Improve Intestinal Motility Disorders in a Thy1-Syn Parkinson's Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Shen,Zhaoqin Wang,Rude Huang,Luyi Wu,Yan Huang,Qin Qi,Rui Zhong,Yiyi Chen,Lingjie Li,Huangan Wu

Oxidative medicine and cellular longevity 2022:4815284 PubMed35726333

2022

PLGA Containing Human Adipose-Derived Stem Cell-Derived Extracellular Vesicles Accelerates the Repair of Alveolar Bone Defects via Transfer of CGRP.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Bo Zhang,Yufan Yang,Bibo Peng,Rui Ye

Journal of neuroinflammation 18:287 PubMed34893074

2021

MicroRNA-155-5p promotes neuroinflammation and central sensitization via inhibiting SIRT1 in a nitroglycerin-induced chronic migraine mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Qianwen Wen,Yunfeng Wang,Qi Pan,Ruimin Tian,Dunke Zhang,Guangcheng Qin,Jiying Zhou,Lixue Chen

Science translational medicine 12: PubMed32641489

2020

Cyclin-dependent-like kinase 5 is required for pain signaling in human sensory neurons and mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Paolo La Montanara,Arnau Hervera,Lucas L Baltussen,Thomas H Hutson,Ilaria Palmisano,Francesco De Virgiliis,Guiping Kong,Jessica Chadwick,Yunan Gao,Katalin Bartus,Qasim A Majid,Nikos Gorgoraptis,Kingsley Wong,Jenny Downs,Tommaso Pizzorusso,Sila K Ultanir,Helen Leonard,Hongwei Yu,David S Millar,Nagy Istvan,Nicholas D Mazarakis,Simone Di Giovanni

Gene 655:1-12 PubMed29474860

2018

Pharmacological targeting of the mammalian clock reveals a novel analgesic for osteoarthritis-induced pain.

Applications

Unspecified application

Species

Unspecified reactive species

Vaskar Das,Ranjan Kc,Xin Li,Disha Varma,Sujun Qiu,Jeffrey S Kroin,Christopher B Forsyth,Ali Keshavarzian,Andre J van Wijnen,Thomas J Park,Gary S Stein,Insug O-Sullivan,Thomas P Burris,Hee-Jeong Im

Scientific reports 8:3220 PubMed29459715

2018

Saliva as a medium to detect and measure biomarkers related to pain.

Applications

Unspecified application

Species

Unspecified reactive species

Hajer Jasim,Anders Carlsson,Britt Hedenberg-Magnusson,Bijar Ghafouri,Malin Ernberg

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com