Rabbit Polyclonal Calcitonin antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 32 publications.
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- International journal of molecular sciences 24:2023Enhanced Intervertebral Disc Repair via Genetically Engineered Mesenchymal Stem Cells with Tetracycline Regulatory System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeji Kim,Seong Bae An,Sang-Hyuk Lee,Jong Joo Lee,Sung Bum Kim,Jae-Cheul Ahn,Dong-Youn Hwang,Inbo HanPubMed 38003216
- Frontiers in neurology 14:11680122023Attenuation of immobilization stress-induced hypertension by temperature-controllable warm needle acupuncture in rats and the peripheral neural mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSe Kyun Bang,Suchan Chang,Su Yeon Seo,Suk-Yun Kang,Seong Jin Cho,Kwang-Ho Choi,Xing Juping,Hee Young Kim,Yeonhee RyuPubMed 37384285
- International journal of molecular sciences 24:2023An Injectable Engineered Cartilage Gel Improves Intervertebral Disc Repair in a Rat Nucleotomy Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBasanta Bhujel,Soon Shim Yang,Hwal Ran Kim,Sung Bum Kim,Byoung-Hyun Min,Byung Hyune Choi,Inbo HanPubMed 36834559
- Brain and behavior 12:e25942022Overexpression of BDNF in the ventrolateral periaqueductal gray regulates the behavior of epilepsy-migraine comorbid rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong Wang,Lu-Lan Fu,Zi-Ru Deng,Juan Zhang,Mei-Dan Zu,Jun-Cang Wu,Yu WangPubMed 35557046
- Neuroscience bulletin 38:386-4022022Referred Somatic Hyperalgesia Mediates Cardiac Regulation by the Activation of Sympathetic Nerves in a Rat Model of Myocardial Ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Cui,Guang Sun,Honglei Cao,Qun Liu,Kun Liu,Shuya Wang,Bing Zhu,Xinyan GaoPubMed 35471719
- Journal of orthopaedic translation 33:153-1612022Biodegradable magnesium implant enhances angiogenesis and alleviates medication-related osteonecrosis of the jaw in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWang-Yong Zhu,Jiaxin Guo,Wei-Fa Yang,Zhuo-Ying Tao,Xinmiao Lan,Leilei Wang,Jiankun Xu,Ling Qin,Yu-Xiong SuPubMed 35415073
- The journal of headache and pain 23:122022Efficacy profile of noninvasive vagus nerve stimulation on cortical spreading depression susceptibility and the tissue response in a rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTzu-Ting Liu,Andreia Morais,Tsubasa Takizawa,Inge Mulder,Bruce J Simon,Shih-Pin Chen,Shuu-Jiun Wang,Cenk Ayata,Jiin-Cherng YenPubMed 35062860
- Bioactive materials 8:95-1082021Magnesium implantation or supplementation ameliorates bone disorder in CFTR-mutant mice through an ATF4-dependent Wnt/β-catenin signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiankun Xu,Peijie Hu,Xiaotian Zhang,Junjiang Chen,Jiali Wang,Jieting Zhang,Ziyi Chen,Mei Kuen Yu,Yiu Wa Chung,Yan Wang,Xiaohu Zhang,Yifeng Zhang,Nianye Zheng,Hao Yao,Jiang Yue,Hsiao Chang Chan,Ling Qin,Ye Chun RuanPubMed 34541389
- European journal of histochemistry : EJH 65:2021Comparison of CGRP distributions in the maxillary sinus and trigeminal ganglion between elderly dentulous and edentulous humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroyuki Matsuda,Iwao Sato,Rieko Asaumi,Takuya Omotehara,Shinichi Kawata,Kenta Nagahori,Zhong-Lian Li,Masahiro ItohPubMed 33787056
- Nature communications 12:4222021Adrenomedullin-CALCRL axis controls relapse-initiating drug tolerant acute myeloid leukemia cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClément Larrue et. al.PubMed 33462236
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Mouse | Predicted |
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-5.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (By similarity).
Alternative names
Calc, Calca, Calcitonin
Recommended products
Rabbit Polyclonal Calcitonin antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 32 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
The immunogen used to raise this antibody has 78% homology with the human CGRP protein. Previous batches of ab47027 have shown to work in human tissue. Please contact Abcam Scientific Support for more information.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CGRP or Calcitonin Gene-Related Peptide is a 37-amino acid neuropeptide with a molecular mass of approximately 3.8 kDa. This peptide exists mainly in two forms alpha-CGRP and beta-CGRP differing slightly in amino acid sequence. CGRP expresses in peripheral and central nervous systems with high levels in the dorsal root ganglia and thyroid gland. It also has a significant presence in the enteric nervous system and some segments of the cardiovascular system. Scientists note that CGRP acts as a potent vasodilator and plays a significant role in transducing pain signals.
- Biological function summary
CGRP contributes to numerous physiological processes including blood flow regulation body temperature control pain transmission and neurogenic inflammation. It acts independently and does not substantially form part of larger protein complexes in its primary activities. Through binding to the CGRP receptor a complex made up of the calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1) CGRP initiates downstream signaling that results in its various biological effects.
- Pathways
Studies place CGRP within the neuropeptide signaling pathway and the vasodilation pathway. It significantly influences the cyclic AMP (cAMP) signaling pathway where it works collaboratively with other neuropeptides like substance P. The interaction with CLR and RAMP1 triggers the production of cAMP which enhances vasodilation and pain transmission processes in the body.
- Associated diseases and disorders
CGRP holds a notable connection to migraines and cardiovascular diseases. Elevated CGRP levels are observed in migraine sufferers and targeted treatments utilizing anti-CGRP antibodies have demonstrated efficacy in reducing migraine frequency. Additionally research links CGRP to blood pressure regulation abnormalities implicating its involvement in hypertension. Both conditions involve complex interactions with other proteins such as substance P and endothelial nitric oxide synthase (eNOS) which modulate vascular tone and inflammation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)
IHC image of CGRP staining in rat dorsal root ganglion formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)
IHC image of CGRP staining in a Rat normal thyroid formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 0.1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)
IHC-P image of CGRP staining on Rat spinal cord sections using ab47027 (1:400). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.
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