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AB314084

Anti-CGRP antibody [BLR169J] - BSA free

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Rabbit Recombinant Monoclonal Calcitonin antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human CALCA aa 50 to C-terminus.

View Alternative Names

CALC1, CALCA, Calcitonin

3 Images
Immunoprecipitation - Anti-CGRP antibody [BLR169J] - BSA free (AB314084)
  • IP

Supplier Data

Immunoprecipitation - Anti-CGRP antibody [BLR169J] - BSA free (AB314084)

Samples : Whole cell lysate (500 µg per IP reaction; 20% of IP loaded) from TT cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-CGRP recombinant monoclonal antibody [BLR169J] (A700-169) used for IP at 20 µl/mg lysate. CGRP was also immunoprecipitated by a second antibody against a differet epitope of CGRP (BL-1966-2B10). For blotting immunoprecipitated CGRP, A700-169 was used at 1 : 1000. Chemiluminescence with an exposure time of 30 seconds.

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Western blot - Anti-CGRP antibody [BLR169J] - BSA free (AB314084)
  • WB

Supplier Data

Western blot - Anti-CGRP antibody [BLR169J] - BSA free (AB314084)

Samples : Whole cell lysate (50 µg) from HeLa, HEK293T, TT, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-CGRP recombinant monoclonal antibody [BLR169J] (A700-169CF) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-CGRP antibody [BLR169J] - BSA free (ab314084) at 1/1000 dilution

Lane 1:

HeLa Whole cell lysate at 50 µg

Lane 2:

HEK293T Whole cell lysate at 50 µg

Lane 3:

TT Whole cell lysate at 50 µg

Lane 4:

Jurkat Whole cell lysate at 50 µg

Lane 5:

Hep-G2 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Western blot - Anti-CGRP antibody [BLR169J] - BSA free (AB314084)
  • WB

Supplier Data

Western blot - Anti-CGRP antibody [BLR169J] - BSA free (AB314084)

Samples : Whole cell lysate (50 µg) from NIH 3T3, CT26, NIH 3T3 + 1 µg purified mouse CGRP, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-CGRP recombinant monoclonal antibody [BLR169J] (A700-169CF) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-CGRP antibody [BLR169J] - BSA free (ab314084) at 1/1000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 50 µg

Lane 2:

CT26 Whole cell lysate at 50 µg

Lane 3:

NIH 3T3 + 1 µg purified mouse CGRP Whole cell lysate at 50 µg

Lane 4:

TCMK-1 Whole cell lysate at 50 µg

Lane 5:

BW5147.3 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR169J

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CALCA aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P01258

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcitonin also known as thyrocalcitonin is a peptide hormone with a molecular mass of about 3.4 kDa. It consists of 32 amino acids and is primarily synthesized in parafollicular cells or C cells of the thyroid gland. This hormone regulates calcium and phosphate homeostasis in the body. It reduces blood calcium levels by inhibiting osteoclast activity in the bones and decreasing reabsorption in the kidneys leading to more calcium excretion. Calcitonin also interacts with its receptor a G protein-coupled receptor found in tissues like bone kidney and brain.
Biological function summary

This hormone plays a significant role in maintaining calcium levels by balancing the actions of parathyroid hormone (PTH). Calcitonin works independently but interacts with the broader system managing bone remodeling through an intricate network. The presence of calcitonin in specialized receptor complexes allows it to exert its effects efficiently in target tissues making it an important player in calcium and phosphorus metabolism.

Pathways

Calcitonin acts within the calcium signaling pathway and the bone resorption pathway. Through these pathways calcitonin works alongside other proteins such as parathyroid hormone (PTH) and calcitonin gene-related peptide (CGRP) maintaining skeletal integrity. It modulates the activity of osteoclasts ensuring that bone resorption activities are counterbalanced by bone formation and influences intracellular calcium concentration by binding to its receptor on target cells.

Calcitonin is linked to conditions such as osteoporosis and medullary thyroid carcinoma. Osteoporosis characterized by decreased bone density and increased fracture risk involves dysregulated calcium and phosphate balance. Proteins like estrogen and growth factors along with calcitonin influence bone health. Medullary thyroid carcinoma a type of thyroid cancer arising from C cells affects calcitonin levels with this hormone serving as a biomarker for diagnosing and monitoring treatment effectiveness. Calcitonin's connection with these diseases highlights its diagnostic and therapeutic potential.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcitonin. Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed : 35324283).. Katacalcin. Katacalcin is a potent plasma calcium-lowering peptide.
See full target information CALCA
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