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Rabbit Recombinant Monoclonal CGRP-I antibody. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF, WB, IHC-Fr and reacts with Human, Rat, Mouse, Recombinant full length protein - Mouse samples. Cited in 4 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (AB283568), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (AB283568), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (AB283568), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (AB283568), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (AB283568), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)IHC-PICC/IFWBIHC-Fr
Human
Not recommended
Tested
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Expected
Tested
Expected
Not recommended
Tested
Rat
Not recommended
Expected
Tested
Expected
Not recommended
Tested
Recombinant full length protein - Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Recombinant full length protein - Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species

Rat

Dilution info

1/100

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species

Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Target data

Function

CGRP1/CALCA is a peptide hormone that induces vasodilation mediated by the CALCRL-RAMP1 receptor complex. Dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP. CGRP1 can also bind and activate CALCR-RAMP1 (AMYR1) receptor complex.

Targets

Calcitonin gene-related peptide 2

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CGRP-I antibody. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF, WB, IHC-Fr and reacts with Human, Rat, Mouse, Recombinant full length protein - Mouse samples. Cited in 4 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23804-95

Purification technique

Affinity purification Protein A

Specificity

Does not work with human IHC-P.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The target designated as CGRP-I and CGRP-II refers to calcitonin gene-related peptide isoforms which play key roles in various physiological functions. CGRP around 37 amino acids long has alternative names such as alpha-CGRP and beta-CGRP occurring mainly in the central nervous system and peripheral nervous tissues. These peptides are potent vasodilators integral to the modulation of blood vessel tone and immune responses. Both isoforms though small in mass allow for significant physiological impacts due to their receptor interactions.

Biological function summary

CGRP-I and CGRP-II contribute to vasodilation and pain transmission in the nervous system. Each isoform can activate specific CGRP receptors mainly located on smooth muscle cells and neurons without forming stable complexes. Their activity involves increasing intracellular cAMP levels facilitating vascular relaxation and modulating nociceptive pathways. Consequently CGRP peptides are essential for conveying pain signals and supporting neurogenic inflammation mechanisms.

Pathways

CGRP-I and CGRP-II serve critical roles in the cardiovascular and nervous system pathways. The cAMP-dependent pathway one of the essential routes of action elevates vasodilation responses where CGRP interacts with the RAMP1 protein a receptor accessory protein. Another pathway involves the modulation of pain where CGRP interacts with other neurotransmitters and proteins such as substance P and neurokinin.

Associated diseases and disorders

CGRP-I and CGRP-II show significant associations with migraines and hypertension. These peptides through their vasodilatory capabilities and nociceptive signaling connect to migraines by interacting with proteins like RAMP1 in trigeminal neurons exacerbating migraine pathology. Furthermore CGRP's role in promoting vasodilation links to hypertension management where altered CGRP activity affects blood pressure regulation. These connections make CGRP-related pathways viable targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Immunohistochemical analysis of paraffin-embedded Mouse spinal cord tissue labelling CGRP-I+CGRP-II with ab283568 at 1/1000 (0.639 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse central termination sensory neurons (PMID: 29634489). The section was incubated with ab283568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Immunohistochemical analysis of paraffin-embedded Rat spinal cord tissue labelling CGRP-I+CGRP-II with ab283568 at 1/1000 (0.639 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat central termination sensory neurons. The section was incubated with ab283568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Immunohistochemical analysis of paraffin-embedded Rat skin tissue labelling CGRP-I+CGRP-II with ab283568 at 1/1000 (0.639 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with ab283568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: no staining on rat skin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling CGRP-I+CGRP-II with ab283568 at 1/1000 (0.639 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the muscularis nerve fibres of mouse colon. The section was incubated with ab283568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spinal cord tissue labeling CGRP-I+CGRP-II with ab283568 at 1/100 (6.39 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green). Positive staining on the dorsal horn of mouse spinal cord is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labelling CGRP-I+CGRP-II with ab283568 at 1/1000 (0.639 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with ab283568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: no staining on mouse skin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spinal cord tissue labeling CGRP-I+CGRP-II with ab283568 at 1/100 (6.39 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green). Positive staining on the dorsal horn of rat spinal cord is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skin tissue labeling CGRP-I+CGRP-II with ab283568 at 1/100 (6.39 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green). The nuclear counterstain was DAPI (Blue).

    Negative control: No staining on rat skin is observed.

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry/ Immunofluorescence - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TT cells labelling CGRP-I+CGRP-II with ab283568 at 1/100 (6.39 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Confocal image showing cytoplasmic staining in TT cell line, and no staining in 293T cell line is observed.

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skin tissue labeling CGRP-I+CGRP-II with ab283568 at 1/100 (6.39 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution (Green). The nuclear counterstain was DAPI (Blue).

    Negative control: No staining on mouse skin is observed.

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Flow Cytometry (Intracellular) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) (Left) / TT (Human thyroid carcinoma epithelial cell) (Right) cells labelling CGRP-I+CGRP-II with ab283568 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, Goat F(ab')2 Anti-Rabbit IgG - H&L (DyLight® 488), pre-adsorbed ab98507) at 1/500 dilution was used as the secondary antibody.

    Negative control: 293T.

  • Western blot - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Western blot - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTFresh lysate of TT cell line was used in this blot.

    Negative control: 293T, Hela, Daudi (HPA database)

    Exposure time: 26 seconds

    All lanes: Western blot - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568) at 1/1000 dilution

    Lane 1: TT (human thyroid carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 10 kDa

    This data was developed using ab283568, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Fresh lysate of TT cell line was used in this blot.

    Negative control: 293T, Hela, Daudi (HPA database)

    Exposure time: 26 seconds

  • Western blot - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568), expandable thumbnail

    Western blot - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody has cross-reaction with mouse CALCB.

    The rec proteins CALCB and CLACA were made in-house.

    CALCB recombinant protein was made in-house and expressed from the E.coli expression system.

    CLACA recombinant protein was expressed from a mammalian - HEK-293 expression system.

    Exposure time: 1 second

    All lanes: Western blot - Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab283568) at 1/1000 dilution

    Lane 1: His-tagged mouse CALCB recombinant protein at 0.01 µg

    Lane 2: Mouse CALCA recombinant protein at 0.01 µg

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 14 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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