AB86073

Anti-ch TOG antibody

Name: Anti-ch TOG antibody (ab86073) | Abcam
Brand: Abcam Limited
SKU: AB86073

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(9 Publications)

Rabbit Polyclonal ch TOG antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human CKAP5.

View Alternative Names

KIAA0097, CKAP5, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, Ch-TOG

2 Images

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Immunoprecipitation - Anti-ch TOG antibody (AB86073)

Immunoprecipitation/ Western Blot of ab86073. Lane 1 : ab86073 at 3µg/mg whole cell lysate. Lane 2 : Control IgG. ab86073 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-ch TOG antibody (ab86073)

Predicted band size: 218 kDa

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Unknown

Western blot - Anti-ch TOG antibody (AB86073)

All lanes:

Western blot - Anti-ch TOG antibody (ab86073) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

mouse NIH3T3 cell lysate at 50 µg

Predicted band size: 218 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CKAP5. The exact immunogen used to generate this antibody is proprietary information.

Q14008

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ch-TOG also known as colonic and hepatic tumor overexpressed gene serves as an important player in microtubule dynamics. With a molecular weight of approximately 217 kDa this protein is an important component in microtubule polymerization. It is expressed in various tissues including the brain and testis demonstrating its diverse role in cellular structure and division processes. As an integral part of the microtubule organizing center ch-TOG directly contributes to the assembly and stability of the mitotic spindle.

Biological function summary

Ch-TOG is highly involved in cell cycle regulation and mitosis. By interacting with microtubules it maintains the proper formation of the mitotic spindle ensuring accurate chromosome segregation in eukaryotic cells. It functions within larger protein complexes such as the Augmin complex which assists in spindle microtubule nucleation. This protein's ability to promote microtubule growth is essential for dynamic cellular events particularly during cell division.

Pathways

Ch-TOG plays a significant role in the cell cycle and mitotic spindle assembly pathways. It closely associates with proteins such as TACC3 and Aurora A kinase which regulate mitotic spindle formation and stability. In the TACC3-Aurora A pathway ch-TOG helps enhance microtubule assembly a process necessary for successful cell proliferation and division. The interaction with these critical proteins illustrates its contribution to maintaining cellular integrity during mitosis.

Ch-TOG links to cancer progression and developmental abnormalities. Its overexpression often appears in various types of cancer such as colorectal and liver cancers due to its role in promoting uncontrolled cell division. In these circumstances ch-TOG interacts with oncogenic pathways potentially involving proteins like p53 to facilitate tumor growth. Additionally mutations or dysregulation in ch-TOG can lead to improper mitotic progression contributing to developmental disorders related to cell division anomalies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as a processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed : 23532825). Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments (PubMed : 27156448).

See full target information CKAP5

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9689 PubMed39516491

2024

HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2.

Applications

Unspecified application

Species

Unspecified reactive species

Venecia Alexandria Valdez,Meisheng Ma,Bernardo Gouveia,Rui Zhang,Sabine Petry

Nature communications 14:289 PubMed36702836

2023

Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG.

Applications

Unspecified application

Species

Unspecified reactive species

Aamir Ali,Chithran Vineethakumari,Cristina Lacasa,Jens Lüders

Cancer cell international 22:238 PubMed35902921

2022

CircPDSS1 promotes the proliferation, invasion, migration, and EMT of breast cancer cell via regulating miR-320c/CKAP5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Liu,Jingyong Song,Yu Kang,Yaojia Wang,Anyue Chen

Cell reports 38:110305 PubMed35081344

2022

Compartmentalized control of Cdk1 drives mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Flavia Serpico,Francesco Febbraro,Caterina Pisauro,Domenico Grieco

Journal of experimental & clinical cancer research : CR 37:247 PubMed30305138

2018

GLI2 promotes cell proliferation and migration through transcriptional activation of ARHGEF16 in human glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dengliang Huang,Yiting Wang,Linlin Xu,Limin Chen,Minzhang Cheng,Wei Shi,Huanting Xiong,Detina Zalli,Shiwen Luo

The Journal of biological chemistry 293:15733-15747 PubMed30054275

2018

The microtubule-associated protein HURP recruits the centrosomal protein TACC3 to regulate K-fiber formation and support chromosome congression.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Zhang,Lora Tan,Qiaoyun Yang,Chenyu Li,Yih-Cherng Liou

Cancer cell 32:444-459.e7 PubMed29017056

2017

Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.

Applications

Unspecified application

Species

Unspecified reactive species

Federico Gulluni,Miriam Martini,Maria Chiara De Santis,Carlo Cosimo Campa,Alessandra Ghigo,Jean Piero Margaria,Elisa Ciraolo,Irene Franco,Ugo Ala,Laura Annaratone,Davide Disalvatore,Giovanni Bertalot,Giuseppe Viale,Anna Noatynska,Mara Compagno,Sara Sigismund,Filippo Montemurro,Marcus Thelen,Fan Fan,Patrick Meraldi,Caterina Marchiò,Salvatore Pece,Anna Sapino,Roberto Chiarle,Pier Paolo Di Fiore,Emilio Hirsch

Molecular biology of the cell 27:1300-9 PubMed26912793

2016

Divergent microtubule assembly rates after short- versus long-term loss of end-modulating kinesins.

Applications

Species

Unspecified reactive species

Linda Wordeman,Justin Decarreau,Juan Jesus Vicente,Michael Wagenbach

The Journal of cell biology 197:141-60 PubMed22472443

2012

Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles.

Applications

Species

Unspecified reactive species

Georg H H Borner,Robin Antrobus,Jennifer Hirst,Gary S Bhumbra,Patrycja Kozik,Lauren P Jackson,Daniela A Sahlender,Margaret S Robinson

View all publications

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Product promise

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Anti-ch TOG antibody

Immunoprecipitation - Anti-ch TOG antibody (AB86073)

Western blot - Anti-ch TOG antibody (AB86073)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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