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AB214295

Anti-CH25H antibody

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(2 Publications)

Rabbit Polyclonal CH25H antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CH25H aa 50-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH, CH25H

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CH25H antibody (AB214295)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CH25H antibody (AB214295)

Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse kidney tissue, blocked with 3% hydrogen peroxide at 37°C for 20 minutes, labeling CH25H using ab214295 at a 1/200 dilution overnight at 4°C, followed by conjugation to the secondary antibody for 90 minutes and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CH25H aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O95992

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CH25H protein also known as cholesterol 25-hydroxylase catalyzes the conversion of cholesterol into 25-hydroxycholesterol. This enzyme has a mass of approximately 33 kDa. It expresses widely in tissues but its level of expression is highest in macrophages and the liver. The protein is part of the enzymatic group that modulates lipid homeostasis and is integral to host defense mechanisms.
Biological function summary

Cholesterol 25-hydroxylase plays a significant role in lipid metabolism and immune regulation. It produces 25-hydroxycholesterol a potent oxysterol which exerts effects on lipid metabolic processes and immune cell signaling. While not part of a large multiprotein complex CH25H's activity impacts cellular cholesterol levels and potentially influences the production of certain signaling molecules.

Pathways

Cholesterol 25-hydroxylase contributes to the regulation of lipid metabolic pathways and innate immune responses. Within the lipid metabolism pathway CH25H interacts with proteins like LXR (liver X receptor) affecting cholesterol homeostasis. The enzyme also influences immune regulation by altering the production of signaling molecules that modulate the interferon response affecting pathways related to inflammation and innate immunity.

Cholesterol 25-hydroxylase has connections to atherosclerosis and viral infections. In the context of atherosclerosis its function in regulating cholesterol levels can influence plaque formation in arteries. The protein's involvement in immune response modulation links it to the body's defense against viral infections. Additionally CH25H's activity interacts with proteins involved in the immune response such as IFN-induced proteins to mount an antiviral state.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed : 9852097). Plays a key role in cell positioning and movement in lymphoid tissues : 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (By similarity). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed : 9852097). As an interferon-stimulated gene, has broad antiviral activities against a wide range of enveloped viruses, such as vesicular stomatitis virus (VSV) and SARS coronavirus-2 (SARS-CoV-2). Its product, 25-hydroxycholesterol, activates the ER-localized enzyme ACAT to induce internalization of accessible cholesterol on the plasma membrane and restricts SARS-CoV-2 S protein-mediated fusion which inhibits virus replication (PubMed : 32944968, PubMed : 33239446). In testis, production of 25-hydroxycholesterol by macrophages plays a role in Leydig cell differentiation (By similarity). Required to restrain inflammation in macrophages : production of 25-hydroxycholesterol protects macrophages from cholesterol overload, thereby preventing mitochondrial DNA release and subsequent activation of the AIM2 inflammasome (By similarity).
See full target information CH25H
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 11:1364380 PubMed38463490

2024

Identification of marker genes for spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Luan,Jiayu Zhang,Yansong Wang

The European journal of neuroscience 50:1759-1778 PubMed30767299

2019

Lipid markers and related transcripts during excitotoxic neurodegeneration in kainate-treated mice.

Applications

Unspecified application

Species

Unspecified reactive species

Farah Chali,Giampaolo Milior,Serge Marty,Mélanie Morin-Brureau,Caroline Le Duigou,Etienne Savary,Corinne Blugeon,Laurent Jourdren,Richard Miles
View all publications
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Product promise

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