AB217808

Anti-CHAC1 antibody

Name: Anti-CHAC1 antibody (ab217808) | Abcam
Brand: Abcam Limited
SKU: AB217808
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal CHAC1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CHAC1 aa 100-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BOTCH, CHAC1, Glutathione-specific gamma-glutamylcyclotransferase 1, Gamma-GCG 1, Blocks Notch protein, Cation transport regulator-like protein 1, Botch

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAC1 antibody (AB217808)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAC1 antibody (AB217808)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling CHAC1 with ab217808 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining

Supplier Data

Western blot - Anti-CHAC1 antibody (AB217808)

All lanes:

Western blot - Anti-CHAC1 antibody (ab217808) at 1/200 dilution

Lane 1:

Mouse brain lysate

Lane 2:

Mouse kidney lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

Predicted band size: 28 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CHAC1 aa 100-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9BUX1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHAC1 also known as cation transport regulator-like protein (CHAC) plays a significant role in various cellular processes. It is a protein with an approximate mass of 27 kDa and is widely expressed in human tissues including the pancreas kidney and liver. This protein functions primarily as a γ-glutamyl cyclotransferase responsible for the cleavage of glutathione into 5-oxo-proline and cysteinylglycine therefore influencing cellular redox status and stress responses.

Biological function summary

CHAC1 acts as an important player in cell death regulation and stress response mechanisms. It participates in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. CHAC1 engages in these processes by interacting with and being regulated by ATF4 a transcription factor activated during cellular stress. It does not function as part of a large complex but has interactions with specific stress-related proteins.

Pathways

CHAC1 is involved in the integrated stress response (ISR) pathway particularly under conditions that elicit ER stress. It has a significant role in the amino acid deprivation response pathway. The ISR pathway links CHAC1 with other proteins such as ATF4 and eIF2α and through these interactions it helps regulate cellular adaptation to stress.

CHAC1 has connections to cancer and neurodegenerative diseases. In certain cancer types CHAC1 gets upregulated linking it to the modulation of oxidative stress and apoptosis which are important processes in tumor development and progression. Additionally in neurodegenerative diseases its role in oxidative stress management potentially contributes to neuronal cell death associating it indirectly with proteins like caspase-3 that are involved in apoptotic pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides (PubMed : 27913623). Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (PubMed : 19109178). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis : acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed : 22445366).

See full target information CHAC1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1589143 PubMed40880648

2025

Polydatin ameliorates hyperhidrosis by targeting Aqp5 in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Feng Chen,Zhi Feng,Feng-Qiang Yu,Rui-Qin Qiu,Xu Li,Jian-Bo Lin

Neoplasma 70:777-786 PubMed38247333

2024

CHAC1 promotes cell ferroptosis and enhances radiation sensitivity in thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlin Yang,Miao Zhang,Wei Xia,Zhongchao Mai,Ying Ye,Bin Zhao,Yanan Song

Allergologia et immunopathologia 51:99-110 PubMed36916093

2023

CHAC1 exacerbates LPS-induced ferroptosis and apoptosis in HK-2 cells by promoting oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Zhou,Hongwei Zhang

FASEB journal : official publication of the Federa 33:13465-13475 PubMed31560857

2019

Redistribution of EC-SOD resolves bleomycin-induced inflammation increased apoptosis of recruited alveolar macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Ayed Allawzi,Ivy McDermott,Cassidy Delaney,Kianna Nguyen,Laith Banimostafa,Ashley Trumpie,Laura Hernandez-Lagunas,Kent Riemondy,Austin Gillen,Jay Hesselberth,Karim El Kasmi,Carmen C Sucharov,William J Janssen,Kurt Stenmark,Russell Bowler,Eva Nozik-Grayck

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CHAC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHAC1 antibody (AB217808)

Western blot - Anti-CHAC1 antibody (AB217808)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise