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AB183852

Anti-CHAC2 antibody

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Rabbit Polyclonal CHAC2 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Glutathione-specific gamma-glutamylcyclotransferase 2 aa 1 to C-terminus.

View Alternative Names

Glutathione-specific gamma-glutamylcyclotransferase 2, Gamma-GCG 2, Cation transport regulator-like protein 2, CHAC2

3 Images
Western blot - Anti-CHAC2 antibody (AB183852)
  • WB

Supplier Data

Western blot - Anti-CHAC2 antibody (AB183852)

All lanes:

Western blot - Anti-CHAC2 antibody (ab183852) at 1/500 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

C8D30 whole cell lysate at 30 µg

Lane 3:

NIH3T3 whole cell lysate at 30 µg

Lane 4:

BCL-1 whole cell lysate at 30 µg

Lane 5:

Raw264.7 whole cell lysate at 30 µg

Lane 6:

C2C12 whole cell lysate at 30 µg

Predicted band size: 21 kDa

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Western blot - Anti-CHAC2 antibody (AB183852)
  • WB

Supplier Data

Western blot - Anti-CHAC2 antibody (AB183852)

All lanes:

Western blot - Anti-CHAC2 antibody (ab183852) at 1/1000 dilution

All lanes:

Human ESC whole cell lysate at 30 µg

Predicted band size: 21 kDa

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Western blot - Anti-CHAC2 antibody (AB183852)
  • WB

Supplier Data

Western blot - Anti-CHAC2 antibody (AB183852)

All lanes:

Western blot - Anti-CHAC2 antibody (ab183852) at 1/500 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Predicted band size: 21 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Glutathione-specific gamma-glutamylcyclotransferase 2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WUX2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein CHAC2 also known as ChaC cation transport regulator homolog 2 is a member of the glutathione-specific gamma-glutamylcyclotransferase family. This protein has a molecular mass of approximately 28 kDa. CHAC2 is mainly expressed in the liver kidney and testis where it plays key roles in cellular processes. Researchers often study CHAC2 for its involvement in modulating cellular redox states and regulating intracellular glutathione levels.
Biological function summary

CHAC2 participates in the degradation of glutathione an essential antioxidant within cells. This protein forms part of a glutathione degradation complex helping to maintain cellular homeostasis by affecting oxidative stress response. By breaking down gamma-glutamyl bonds in glutathione CHAC2 influences many cellular functions such as cell proliferation and apoptosis.

Pathways

CHAC2 significantly impacts oxidative stress and apoptosis pathways. In these routes CHAC2's activity interlinks closely with the regulation of intracellular reactive oxygen species (ROS) levels. CHAC1 a homolog of CHAC2 shares similarities in the pathways suggesting a level of redundancy or cooperative functions between these proteins particularly in stress response mechanisms.

CHAC2 associates with conditions featuring oxidative stress imbalances like cancer and neurodegenerative disorders. The modulation of glutathione levels by CHAC2 has implications in cancer therapy where oxidative stress could affect cell survival. Connections with proteins like CHAC1 highlight similar pathways in disease contexts suggesting potential combinatorial therapeutic strategies targeting related pathways in oxidative stress-induced conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides.
See full target information Glutathione-specific gamma-glutamylcyclotransferase 2
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