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AB169249

Anti-CHAMP antibody

5

(3 Reviews)

|

(1 Publication)

Mouse Polyclonal CHAMP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CHAMP1.

View Alternative Names

C13orf8, CAMP, CHAMP, KIAA1802, ZNF828, CHAMP1, Chromosome alignment-maintaining phosphoprotein 1, Zinc finger protein 828

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-CHAMP antibody (AB169249)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CHAMP antibody (AB169249)

Immunofluorescent analysis of HeLa cells labeling CHAMP with ab169249 at 10 μg/ml.

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CHAMP1.

Q96JM3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHAMP also known as Chromatin Associated Module Protein serves an important role in chromatin remodeling. Its molecular mass is approximately 75 kDa. This protein localizes mainly in the nucleus where it assists in altering chromatin structure to regulate DNA access. CHAMP facilitates the binding of transcription factors by repositioning nucleosomes which is essential for gene expression and DNA repair processes.
Biological function summary

CHAMP plays a role in the regulation of gene expression and impacts cellular differentiation and proliferation. It acts as part of a larger chromatin remodeling complex that includes several other proteins enabling the coordination of multiple functions necessary for cellular homeostasis. Its activity ensures that specific genes are either activated or repressed as the cell requires contributing to the diversity of cellular functions.

Pathways

CHAMP contributes to key signaling cascades such as the Wnt signaling and Notch signaling pathways. In the Wnt pathway its modification of chromatin accessibility allows transcription factors like TCF/LEF to bind DNA and activate transcription. The protein shows interactions with core components like β-catenin in Wnt signaling and in the Notch pathway it influences cellular fate decisions by impacting expression of target genes critical for development.

Improper function of CHAMP has connections to cancer and neurological disorders. Altered activity can lead to dysregulation of gene expression promoting tumorigenesis where it often associates with increased levels of β-catenin an important protein in cancer development. Similarly in neurological disorders its failure in chromatin modification affects the expression of genes essential for neural function sometimes involving interactions with proteins like NICD in Notch-related processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
See full target information CHAMP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of surgical pathology 40:224-35 PubMed26501226

2015

A Molecular Study of Pediatric Spindle and Sclerosing Rhabdomyosarcoma: Identification of Novel and Recurrent VGLL2-related Fusions in Infantile Cases.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Alaggio,Lei Zhang,Yun-Shao Sung,Shih-Chiang Huang,Chun-Liang Chen,Gianni Bisogno,Angelica Zin,Narasimhan P Agaram,Michael P LaQuaglia,Leonard H Wexler,Cristina R Antonescu
View all publications
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Product promise

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