Rabbit Polyclonal CHCH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHURC1 aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CHURC1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WUH1
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Orangutan | Dilution info - | Notes - |
Target data
Function
Transcriptional activator that mediates FGF signaling during neural development (By similarity). Plays a role in the regulation of cell movement (By similarity). Isoform 4. Does not bind DNA by itself.
Alternative names
C14orf52, CHCH, My015, CHURC1, Protein Churchill
Recommended products
Rabbit Polyclonal CHCH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHURC1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CHURC1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WUH1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CHCH domain-containing protein also known as CHCHD plays a mechanical role within the cell by being involved in mitochondrial processes. It has a mass of approximately 15 kDa. This protein is expressed in various tissues with high levels in the heart and skeletal muscles. It localizes to the mitochondria where it interacts with other mitochondrial proteins to maintain cellular function.
- Biological function summary
The CHCHD protein participates in the assembly and stabilization of protein complexes within the mitochondria. It serves as a component of the mitochondrial intermembrane space bridging complex (MIB) facilitating electron transfer processes necessary for cellular respiration. The stability of the complex is essential for maintaining the proper mitochondrial bioenergetics that influence overall cellular health.
- Pathways
The CHCHD protein is an important player in mitochondrial respiration and apoptosis pathways. It interacts with cytochrome c during the electron transport chain which is important for ATP production. This protein also relates to apoptosis through interactions that influence the release of pro-apoptotic factors like c-caspases supporting the balance between cell survival and programmed cell death.
- Associated diseases and disorders
The CHCHD protein associates with neurodegenerative diseases such as Parkinson's disease where its dysfunction contributes to mitochondrial abnormalities. It also connects with cardiac disorders since it influences the mitochondrial pathways important for heart function. In Parkinson's disease alterations in interactions between CHCHD and proteins like CHCHD2 have been implicated highlighting the protein's role in mitochondrial health and disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-CHCH antibody (ab243505)
PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma derived from bone) cells stained for CHCH (green) using ab243505 at 4 μg/ml in ICC/IF analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHCH antibody (ab243505)
Formalin-fixed, paraffin-embedded human colon tissue stained for CHCH using ab243505 at 1/50 dilution in immunohistochemical analysis.
Western blot - Anti-CHCH antibody (ab243505)
All lanes: Western blot - Anti-CHCH antibody (ab243505) at 0.4 µg/mL
Lane 1: Empty vector transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2: HEK-293T overexpressing CHCH with C-terminal myc-DDk tag (~3.1 kDa).
Predicted band size: 13 kDa
Downloads
Product protocols
- Visit the general protocols
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