AB180583

Anti-CHCHD1 antibody [EPR12253(2)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CHCHD1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C10orf34, MRPS37, CHCHD1, Small ribosomal subunit protein mS37, Coiled-coil-helix-coiled-coil-helix domain-containing protein 1, Nuclear protein C2360, MRP-S37

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CHCHD1 antibody [EPR12253(2)] (AB180583)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHCHD1 antibody [EPR12253(2)] (AB180583)

Immunocytochemical staining of paraformaldehyde fixed A431 cells labeling CHCHD1 using ab180583 at 1/100 dilution and Goat anti rabbit IgG (Dylight 488) at 1/200. Counterstained with DAPI.

Supplier Data

Western blot - Anti-CHCHD1 antibody [EPR12253(2)] (AB180583)

All lanes:

Western blot - Anti-CHCHD1 antibody [EPR12253(2)] (ab180583) at 1/1000 dilution

Lane 1:

HT-1376 cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 13 kDa

false

Carrier free

Anti-CHCHD1 antibody [EPR12253(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12253(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHCHD1 also known as Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 1 is a protein with a molecular mass of approximately 13 kDa. It is localized mainly in the mitochondria where it plays an important role in maintaining mitochondrial function. Expression of CHCHD1 is found in various tissues with an increased presence in the heart skeletal muscle and brain which indicates its functional relevance in these energy-demanding tissues.

Biological function summary

The coiled-coil structure of CHCHD1 participates in protein-protein interactions within the cell. It is a component of a larger protein complex in the mitochondrial intermembrane space which assists in maintaining the stability and function of mitochondrial proteins. CHCHD1 contributes to the regulation of mitochondrial biogenesis and dynamics affecting cellular energy production and overall metabolism.

Pathways

Studies show that CHCHD1 is involved in the mitochondrial oxidative phosphorylation pathway. It assists in regulating electron transport chain efficiency influencing ATP production. CHCHD1 also interacts with cytochrome c an important player in the electron transport chain highlighting its role in energy metabolism. These interactions ensure cells produce enough energy to support vital functions.

Research connects CHCHD1 to neurodegenerative diseases like Parkinson's and amyotrophic lateral sclerosis (ALS). Altered CHCHD1 function can disrupt mitochondrial homeostasis contributing to the pathophysiology of these conditions. Interaction with proteins such as alpha-synuclein in Parkinson's or SOD1 in ALS links CHCHD1 to these disorders emphasizing its importance in disease progression and as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information CHCHD1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 222: PubMed37200023

2023

Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters.

Applications

Unspecified application

Species

Unspecified reactive species

Femmy C Stempels,Muwei Jiang,Harry M Warner,Magda-Lena Moser,Maaike H Janssens,Sjors Maassen,Iris H Nelen,Rinse de Boer,William F Jiemy,David Knight,Julian Selley,Ronan O'Cualain,Maksim V Baranov,Thomas C Q Burgers,Roberto Sansevrino,Dragomir Milovanovic,Peter Heeringa,Matthew C Jones,Rifka Vlijm,Martin Ter Beest,Geert van den Bogaart

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CHCHD1 antibody [EPR12253(2)]

Immunocytochemistry/ Immunofluorescence - Anti-CHCHD1 antibody [EPR12253(2)] (AB180583)

Western blot - Anti-CHCHD1 antibody [EPR12253(2)] (AB180583)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise