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AB250231

Anti-CHCHD1 antibody [EPR12253(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CHCHD1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C10orf34, MRPS37, CHCHD1, Small ribosomal subunit protein mS37, Coiled-coil-helix-coiled-coil-helix domain-containing protein 1, Nuclear protein C2360, MRP-S37

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CHCHD1 antibody [EPR12253(2)] - BSA and Azide free (AB250231)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHCHD1 antibody [EPR12253(2)] - BSA and Azide free (AB250231)

This data was developed using ab180583, the same antibody clone in a different buffer formulation.

Immunocytochemical staining of paraformaldehyde fixed A431 cells labeling CHCHD1 using ab180583 at 1/100 dilution and Goat anti rabbit IgG (Dylight 488) at 1/200. Counterstained with DAPI.

Western blot - Anti-CHCHD1 antibody [EPR12253(2)] - BSA and Azide free (AB250231)
  • WB

Supplier Data

Western blot - Anti-CHCHD1 antibody [EPR12253(2)] - BSA and Azide free (AB250231)

This data was developed using ab180583, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CHCHD1 antibody [EPR12253(2)] (<a href='/en-us/products/primary-antibodies/chchd1-antibody-epr122532-ab180583'>ab180583</a>) at 1/1000 dilution

Lane 1:

HT-1376 cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12253(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250231 is the carrier-free version of ab180583.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHCHD1 also known as Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 1 is a protein with a molecular mass of approximately 13 kDa. It is localized mainly in the mitochondria where it plays an important role in maintaining mitochondrial function. Expression of CHCHD1 is found in various tissues with an increased presence in the heart skeletal muscle and brain which indicates its functional relevance in these energy-demanding tissues.
Biological function summary

The coiled-coil structure of CHCHD1 participates in protein-protein interactions within the cell. It is a component of a larger protein complex in the mitochondrial intermembrane space which assists in maintaining the stability and function of mitochondrial proteins. CHCHD1 contributes to the regulation of mitochondrial biogenesis and dynamics affecting cellular energy production and overall metabolism.

Pathways

Studies show that CHCHD1 is involved in the mitochondrial oxidative phosphorylation pathway. It assists in regulating electron transport chain efficiency influencing ATP production. CHCHD1 also interacts with cytochrome c an important player in the electron transport chain highlighting its role in energy metabolism. These interactions ensure cells produce enough energy to support vital functions.

Research connects CHCHD1 to neurodegenerative diseases like Parkinson's and amyotrophic lateral sclerosis (ALS). Altered CHCHD1 function can disrupt mitochondrial homeostasis contributing to the pathophysiology of these conditions. Interaction with proteins such as alpha-synuclein in Parkinson's or SOD1 in ALS links CHCHD1 to these disorders emphasizing its importance in disease progression and as a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information CHCHD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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