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AB220688

Anti-CHCHD2 antibody

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(4 Publications)

Rabbit Polyclonal CHCHD2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CHCHD2 aa 1-50.

View Alternative Names

C7orf17, AAG10, CHCHD2, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, Aging-associated gene 10 protein, HCV NS2 trans-regulated protein, NS2TP

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CHCHD2 antibody (AB220688)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHCHD2 antibody (AB220688)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cells labeling CHCHD2 with ab220688 at 4 μg/mL (green).

Western blot - Anti-CHCHD2 antibody (AB220688)
  • WB

Supplier Data

Western blot - Anti-CHCHD2 antibody (AB220688)

All lanes:

Western blot - Anti-CHCHD2 antibody (ab220688) at 1/100 dilution

Lane 1:

RT-4 cell line lysate

Lane 2:

U-251 MG cell line lysate

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CHCHD2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6H1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHCHD2 also known as coiled-coil-helix-coiled-coil-helix domain-containing protein 2 is encoded by the CHCHD2 gene. Weighing approximately 15.5 kDa CHCHD2 is located in the mitochondrial intermembrane space. Expression of CHCHD2 occurs in various tissues with higher levels in the brain and heart. Its mechanical action involves interactions with mitochondrial proteins to help stabilize the structure and function within the cell mitochondria.
Biological function summary

CHCHD2 plays a role in mitochondrial respiratory function and cellular energy metabolism. This protein partners with other mitochondrial proteins forming complexes that maintain mitochondrial integrity and proper energy production. CHCHD2 contributes to processes such as oxidative phosphorylation by interacting with the mitochondrial respiratory chain impacting ATP generation and cellular energy dynamics.

Pathways

Interactions with other proteins such as CHCHD10 position CHCHD2 in significant signaling and energy pathways like the respiratory electron transport chain. CHCHD2's role within oxidative phosphorylation and mitochondrial biogenesis pathways is fundamental. These pathways are central to energy production and are interconnected with other mitochondrial proteins like complex IV members ensuring efficient energy output within cells.

Mutations in CHCHD2 associate with Parkinson’s disease and other neurodegenerative conditions. These mutations can disrupt mitochondrial function leading to energy deficits and cellular stress. CHCHD2 is also linked to proteins involved in neurodegeneration such as alpha-synuclein suggesting a complex interplay that may contribute to the pathogenesis of these diseases. Understanding these associations can inform therapeutic strategies targeting CHCHD2 dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen) (PubMed : 23303788).
See full target information CHCHD2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115687 PubMed40343798

2025

A retinoic acid:YAP1 signaling axis controls atrial lineage commitment.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Abraham,Aleksandra Kostina,Brett Volmert,Thomas Roule,Ling Huang,Jingting Yu,April E Williams,Emily Megill,Aidan Douglas,Olivia M Pericak,Alex Morris,Eleonora Stronati,Arantza Larrinaga-Zamanillo,Raquel Fueyo,Mikel Zubillaga,Mark D Andrake,Naiara Akizu,Aitor Aguirre,Conchi Estaras

Human molecular genetics 31:3987-4005 PubMed35786718

2022

Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa R Kee,Jessica L Wehinger,Pamela Espinoza Gonzalez,Eric Nguyen,Kyle C McGill Percy,Sophia A Khan,Dale Chaput,Xinming Wang,Tian Liu,David E Kang,Jung-A A Woo

Human molecular genetics 29:2788-2802 PubMed32898862

2020

Gene expression profiles complement the analysis of genomic modifiers of the clinical onset of Huntington disease.

Applications

Unspecified application

Species

Unspecified reactive species

Galen E B Wright,Nicholas S Caron,Bernard Ng,Lorenzo Casal,William Casazza,Xiaohong Xu,Jolene Ooi,Mahmoud A Pouladi,Sara Mostafavi,Colin J D Ross,Michael R Hayden

Cancer cell international 19:313 PubMed31787850

2019

Long noncoding RNA DLX6-AS1 promotes neuroblastoma progression by regulating miR-107/BDNF pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huan-Yu Zhang,Mao-Qing Xing,Jing Guo,Jin-Chuan Zhao,Xin Chen,Zhong Jiang,Hong Zhang,Qian Dong
View all publications
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