Anti-Chd1 antibody

• WB

Supplier Data

Western blot - Anti-Chd1 antibody (AB242098)

All lanes:

Western blot - Anti-Chd1 antibody (ab242098) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 197 kDa

true

Exposure time: 3s

• ChIP/Chip

Unknown

ChIP on chip - Anti-Chd1 antibody (AB242098)

ab242098 (10 μg) was used to immunoprecipitate chromatin from K562 cells according to the protocol of Ren et al (Genes Dev. 2002 16 : 245-256).

(A) Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites.

(B) As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Chd1 ChIP, normal rabbit IgG showed little enrichment.

• IP

Supplier Data

Immunoprecipitation - Anti-Chd1 antibody (AB242098)

Chd1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242098 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab242098 at 1 μg/ml.

Lane 1 : ab242098 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Chd1 antibody (ab242098)

Predicted band size: 197 kDa

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