Rabbit Recombinant Monoclonal CHD1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant full length protein - Human, Human samples.
Images
![Western blot - Anti-Chd1 antibody [EPR28727-78] (AB318138), expandable thumbnail](https://content.abcam.com/products/images/chd1-antibody-epr28727-78-ab318138--western-blot-img432635.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Chd1 antibody [EPR28727-78] (AB318138)")
![Immunocytochemistry/ Immunofluorescence - Anti-Chd1 antibody [EPR28727-78] (AB318138), expandable thumbnail](https://content.abcam.com/products/images/chd1-antibody-epr28727-78-ab318138--immunocytochemistry-immunofluorescence-img432574.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Chd1 antibody [EPR28727-78] (AB318138)")
![Western blot - Anti-Chd1 antibody [EPR28727-78] (AB318138), expandable thumbnail](https://content.abcam.com/products/images/chd1-antibody-epr28727-78-ab318138--western-blot-img432555.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Chd1 antibody [EPR28727-78] (AB318138)")
![Flow Cytometry (Intracellular) - Anti-Chd1 antibody [EPR28727-78] (AB318138), expandable thumbnail](https://content.abcam.com/products/images/chd1-antibody-epr28727-78-ab318138--flow-cytometry-intracellular-img432617.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Chd1 antibody [EPR28727-78] (AB318138)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IP | IHC-P | ChIP-seq | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant full length protein - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
ChIP-seq
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant full length protein - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3 (PubMed:18042460, PubMed:28866611). Required for maintaining open chromatin and pluripotency in embryonic stem cells (By similarity).
Alternative names
Chromodomain-helicase-DNA-binding protein 1, CHD-1, ATP-dependent helicase CHD1, CHD1
Recommended products
Rabbit Recombinant Monoclonal CHD1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant full length protein - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28727-78
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Chd1 antibody [EPR28727-78] (ab318138), expandable thumbnail](https://content.abcam.com/products/images/chd1-antibody-epr28727-78-ab318138--western-blot-img432635.jpg "Western blot - Anti-Chd1 antibody [EPR28727-78] (ab318138)")
Western blot - Anti-Chd1 antibody [EPR28727-78] (ab318138)
Chd1 Western blot staining using rabbit Anti-Chd1 antibody
In Western blot, ab318138 was shown to bind specifically to Chd1. Target of interest was observed at 250 kDa in wild-type HEK-293T cell lysates (lane 1) with no signal observed at this size in CHD1 knockout cell line Human CHD1 knockout HEK-293T cell line ab266512 (knockout cell lysate 263151) (lane 2).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Chd1 antibody [EPR28727-78] (ab318138) at 1/1000 dilution
Lane 1: Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: CHD1 knockout HEK-293T whole cell lysate at 20 µg with NFDM/TBST
Lane 3: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 250 kDa, 124 kDa
Exposure time: 180s
![Immunocytochemistry/ Immunofluorescence - Anti-Chd1 antibody [EPR28727-78] (ab318138), expandable thumbnail](https://content.abcam.com/products/images/chd1-antibody-epr28727-78-ab318138--immunocytochemistry-immunofluorescence-img432574.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Chd1 antibody [EPR28727-78] (ab318138)")
Immunocytochemistry/ Immunofluorescence - Anti-Chd1 antibody [EPR28727-78] (ab318138)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CHD1 KO HEK293T (CHD1 knockout human embryonic kidney epithelial cell) Human CHD1 knockout HEK-293T cell line ab266512 cells labelling Chd1 with ab318138 at 1/50 (10.58 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing nuclear staining in wildtype HEK-293T cells (shown in green), and negative staining in CHD1 knockout HEK-293T cells. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Western blot - Anti-Chd1 antibody [EPR28727-78] (ab318138), expandable thumbnail](https://content.abcam.com/products/images/chd1-antibody-epr28727-78-ab318138--western-blot-img432555.jpg "Western blot - Anti-Chd1 antibody [EPR28727-78] (ab318138)")
Western blot - Anti-Chd1 antibody [EPR28727-78] (ab318138)
This antibody does not cross-react with human Chd2.
In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Chd1 antibody [EPR28727-78] (ab318138) at 1/1000 dilution
Lane 1: His-tagged human Chd1 protein at 10 ng with NFDM/TBST
Lane 2: His-tagged human Chd2 protein at 10 ng with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 25 kDa
Exposure time: 180s
![Flow Cytometry (Intracellular) - Anti-Chd1 antibody [EPR28727-78] (ab318138), expandable thumbnail](https://content.abcam.com/products/images/chd1-antibody-epr28727-78-ab318138--flow-cytometry-intracellular-img432617.jpg "Flow Cytometry (Intracellular) - Anti-Chd1 antibody [EPR28727-78] (ab318138)")
Flow Cytometry (Intracellular) - Anti-Chd1 antibody [EPR28727-78] (ab318138)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized CHD1 konckout HEK293T (Right) / Parental HEK293T(EDWT04) (Left) cells labelling Chd1 with ab318138 at 1/500 dilution (0.1ug) /Magenta (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
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