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Rabbit Recombinant Monoclonal CHD1L antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 4 publications.

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Images

Western blot - Anti-CHD1L antibody [EPR14515(2)] (AB197019), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [EPR14515(2)] (AB197019), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [EPR14515(2)] (AB197019), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CHD1L antibody [EPR14515(2)] (AB197019), expandable thumbnail
  • Immunoprecipitation - Anti-CHD1L antibody [EPR14515(2)] (AB197019), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/520
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

ATP-dependent chromatin remodeler that mediates chromatin-remodeling following DNA damage (PubMed:19661379, PubMed:29220652, PubMed:29220653, PubMed:33357431, PubMed:34210977, PubMed:34486521, PubMed:34874266). Recruited to DNA damage sites through interaction with poly-ADP-ribose: specifically recognizes and binds histones that are poly-ADP-ribosylated on serine residues in response to DNA damage (PubMed:19661379, PubMed:29220652, PubMed:29220653, PubMed:34486521, PubMed:34874266). Poly-ADP-ribose-binding activates the ATP-dependent chromatin remodeler activity, thereby regulating chromatin during DNA repair (PubMed:19661379, PubMed:29220652, PubMed:29220653, PubMed:34486521, PubMed:34874266). Catalyzes nucleosome sliding away from DNA breaks in an ATP-dependent manner (PubMed:19661379, PubMed:29220652, PubMed:29220653). Chromatin remodeling activity promotes PARP2 removal from chromatin (PubMed:33275888).

Alternative names

ALC1, CHD1L, Chromodomain-helicase-DNA-binding protein 1-like, Amplified in liver cancer protein 1

Recommended products

Rabbit Recombinant Monoclonal CHD1L antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14515(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The CHD1L gene encodes a protein known as chromodomain helicase DNA-binding protein 1-like. This protein plays a role in chromatin remodeling using the energy from ATP hydrolysis to alter chromatin structure thereby facilitating processes such as DNA repair and transcription. The mass of CHD1L is approximately 120 kDa. This protein is expressed in various tissues with a notable presence in liver and cancerous tissues indicating its potential involvement in cellular proliferation and repair mechanisms.

Biological function summary

The function of CHD1L is significant in genomic stability and response to DNA damage. It acts as a part of the chromatin remodeling complex and contributes to the regulation of chromatin dynamics affecting gene expression and repair pathways. CHD1L interacts with other proteins involved in DNA repair ensuring the cell maintains genetic integrity under stress conditions.

Pathways

CHD1L is involved in the DNA damage response and chromatin organization pathways. It interacts with RAD51-associated protein which is important for homologous recombination repair and collaborates with proteins in the pathway to restore DNA integrity after damage. CHD1L's activity in these pathways highlights its role in maintaining cellular homeostasis and preventing genomic instability.

Associated diseases and disorders

CHD1L has been implicated in hepatocellular carcinoma and ovarian cancer. The overexpression of CHD1L in such cancers suggests a role in cancer progression by promoting cell proliferation and survival. Additionally its interaction with HNF4A a protein involved in liver cancer accentuates its impact on oncogenic pathways. Understanding CHD1L in these contexts could offer insights into therapeutic targets for cancer treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-CHD1L antibody [EPR14515(2)] (ab197019), expandable thumbnail

    Western blot - Anti-CHD1L antibody [EPR14515(2)] (ab197019)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CHD1L antibody [EPR14515(2)] (ab197019) at 1/5000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

    Lane 2: A549 (Human lung carcinoma) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 101 kDa

    Observed band size: 101 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [EPR14515(2)] (ab197019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [EPR14515(2)] (ab197019)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling CHD1L with ab197019 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nuclear and weakly cytoplasm staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [EPR14515(2)] (ab197019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [EPR14515(2)] (ab197019)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CHD1L with ab197019 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nuclear and weakly cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CHD1L antibody [EPR14515(2)] (ab197019), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CHD1L antibody [EPR14515(2)] (ab197019)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CHD1L with ab197019 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Nuclear and weakly cytoplasm staining on HeLa cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab197019 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunoprecipitation - Anti-CHD1L antibody [EPR14515(2)] (ab197019), expandable thumbnail

    Immunoprecipitation - Anti-CHD1L antibody [EPR14515(2)] (ab197019)

    CHD1L was immunoprecipitated from 293 (Human embryonic kidney) whole cell extract with ab197019 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab197019 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: 293 whole cell extract (Input) 10 μg. Lane 2: ab197019 IP in 293 whole cell extract. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab197019 in 293 whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CHD1L antibody [EPR14515(2)] (ab197019)

    Predicted band size: 101 kDa

  • Flow Cytometry (Intracellular) - Anti-CHD1L antibody [EPR14515(2)] (ab197019), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CHD1L antibody [EPR14515(2)] (ab197019)

    Intracellular flow cytometric analysis of HeLa (Human epithelial cells from cervix adenocarcinoma)cells labeling CHD1L with ab197019 at 1/520 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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