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AB308222

Anti-CHD2 antibody [HL2066]

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Rabbit Recombinant Monoclonal CHD2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHD2.

View Alternative Names

Chromodomain-helicase-DNA-binding protein 2, CHD-2, ATP-dependent helicase CHD2, CHD2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD2 antibody [HL2066] (AB308222)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD2 antibody [HL2066] (AB308222)

Immunohistochemical analysis of paraffin-embedded mouse tissues labelling CHD2 with ab308222 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-CHD2 antibody [HL2066] (AB308222)
  • WB

Supplier Data

Western blot - Anti-CHD2 antibody [HL2066] (AB308222)

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD2 antibody [HL2066] (ab308222) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Lane 1:

293T cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Lane 3:

HeLa cell lysate at 30 µg

Lane 4:

HepG2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-CHD2 antibody [HL2066] (AB308222)
  • WB

Supplier Data

Western blot - Anti-CHD2 antibody [HL2066] (AB308222)

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD2 antibody [HL2066] (ab308222) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CHD2 antibody [HL2066] (ab308222) at 1/1000 dilution

Lane 1:

Neuro2A cell lysate at 30 µg

Lane 2:

Rat2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2066

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CHD2. The exact immunogen used to generate this antibody is proprietary information.

O14647

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CHD2 protein also known as Chromodomain-Helicase-DNA-binding protein 2 acts as an ATP-dependent chromatin remodeler. This protein has a mass of approximately 210 kDa. CHD2 is expressed in various tissues with higher expression levels noted in the brain and skeletal muscle. As a part of the chromatin remodeling family CHD2 plays a role in modifying the structure of chromatin to affect gene expression.
Biological function summary

CHD2 contributes to DNA repair and transcription regulation by modifying chromatin structure. It is a part of the CHD family of chromatin remodelers which influences nucleosome positioning and accessibility. By facilitating transcription CHD2 impacts gene expression necessary for neural development and other processes. CHD2 functions as a monomer and does not form larger protein complexes.

Pathways

CHD2 influences gene expression regulation and neural development. It plays a role in the transcriptional regulation pathway by interacting with chromatin to control the accessibility of transcription factors. The protein links with others like CHD3 and CHD4 within this pathway to coordinate chromatin conformation changes necessary for proper transcription processes.

The CHD2 protein relates to epileptic encephalopathy and neurodevelopmental disorders. Mutations in the CHD2 gene associate with these conditions affecting neurological function and development. CHD2 mutations often coexist with other genetic factors involving proteins like SCN1A contributing to the complex nature of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-binding helicase that specifically binds to the promoter of target genes, leading to chromatin remodeling, possibly by promoting deposition of histone H3.3. Involved in myogenesis via interaction with MYOD1 : binds to myogenic gene regulatory sequences and mediates incorporation of histone H3.3 prior to the onset of myogenic gene expression, promoting their expression (By similarity).
See full target information CHD2
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Product promise

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