AB248690

Anti-CHD3 antibody [EPNCIR110] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CHD3 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse samples. Cited in 1 publication.

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110] - BSA and Azide free (AB248690)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110] - BSA and Azide free (AB248690)

This data was developed using ab134125, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Mouse spleen tissue labelling CHD3 with ab134125 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110] - BSA and Azide free (AB248690)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110] - BSA and Azide free (AB248690)

This data was developed using ab134125, the same antibody clone in a different buffer formulation.Immunofluorescent staining of 3T3 cells labelling CHD3 with ab134125 at 1/100.

Unknown

Western blot - Anti-CHD3 antibody [EPNCIR110] - BSA and Azide free (AB248690)

This data was developed using ab134125, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CHD3 antibody [EPNCIR110] (<a href='/en-us/products/primary-antibodies/chd3-antibody-epncir110-ab134125'>ab134125</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 226 kDa

Observed band size: 260 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR110

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab248690 is the carrier-free version of ab134125.

Species reactivity
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Collaborations
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHD3 also known as Chromodomain Helicase DNA Binding Protein 3 is a protein involved in transcription regulation. The protein has a molecular mass of approximately 220 kDa. CHD3 is a member of the chromatin remodeler family and is expressed widely throughout various tissues. It contains dual chromodomains a helicase/ATPase domain and a DNA-binding domain enabling it to remodel chromatin structure by altering nucleosome positioning on DNA.

Biological function summary

CHD3 plays a significant role in modifying chromatin architecture and regulating the accessibility of the DNA to transcription factors. It functions as part of the NuRD (Nucleosome Remodeling and Deacetylase) complex which combines the features of ATP-dependent chromatin remodeling and histone deacetylase activity. CHD3 helps remodel nucleosomes and remove acetyl groups from histones which leads to chromatin condensation and transcriptional repression. Its activity influences genes involved in development differentiation and cell cycle control.

Pathways

CHD3's function integrates into pathways that control gene expression and DNA repair. It plays a role in the transcriptional regulation pathway working alongside proteins like HDAC1 and NuRD components. Additionally CHD3 participates in the DNA damage response cooperating with proteins such as CHD4 and RBBP4 to ensure genomic stability. These interactions and functions place CHD3 at a strategic position within cellular mechanisms maintaining transcription fidelity and genomic integrity.

CHD3 associations with cancer and developmental disorders stand out. Abnormal expression or mutations in CHD3 can contribute to tumorigenesis as it can affect the expression and repression of oncogenes and tumor suppressor genes. Furthermore CHD3 mutations have been linked to Snijders Blok-Campeau syndrome a neurodevelopmental disorder. The interaction between CHD3 and other proteins like HDAC1 and CHD4 plays a critical role in the manifestation of these conditions influencing pathways that drive disease progression and phenotype expression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information CHD3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4467 PubMed37491377

2023

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhong,Xujia Wu,Yixin Gao,Junju Chen,Maolei Zhang,Huangkai Zhou,Jia Yang,Feizhe Xiao,Xuesong Yang,Nunu Huang,Haoyue Qi,Xiuxing Wang,Fan Bai,Yu Shi,Nu Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CHD3 antibody [EPNCIR110] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110] - BSA and Azide free (AB248690)

Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110] - BSA and Azide free (AB248690)

Western blot - Anti-CHD3 antibody [EPNCIR110] - BSA and Azide free (AB248690)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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