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AB70469

Anti-CHD4 antibody [3F2/4]

5

(8 Reviews)

|

(65 Publications)

Mouse Monoclonal CHD4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 65 publications.

View Alternative Names

ATP-dependent chromatin remodeler CHD4, Chromo domain-containing protein 4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, CHD-4, CHD4

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD4 antibody [3F2/4] (AB70469)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD4 antibody [3F2/4] (AB70469)

IHC image of CHD4 staining in human breast carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70469, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Carrier free

    Anti-CHD4 antibody [3F2/4] - ChIP Grade - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3F2/4

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHD4 also known as CDH4 is a protein that functions as part of the nucleosome remodeling and deacetylase (NuRD) complex. CHD4 has a molecular mass of approximately 218 kDa. The protein contains a chromo domain a helicase domain and two PHD-type zinc fingers. It is expressed ubiquitously in human tissues with higher expression levels seen in the spleen and fetal brain. This protein plays an essential role in DNA transcription regulation by modifying chromatin structure.
Biological function summary

CHD4 acts within the NuRD complex to mediate transcriptional repression. This complex combines chromatin remodeling with histone deacetylation to alter chromatin dynamics affecting gene expression. CHD4's involvement in the complex facilitates the static organization of chromatin therefore contributing to transcriptional silencing of specific gene sets. The protein's activity is often in response to environmental and developmental cues playing an integral role in cellular differentiation and proliferation processes.

Pathways

CHD4 connects closely with DNA damage repair and cell cycle regulation pathways. It participates actively in the DNA damage response pathway by facilitating the repair of double-strand breaks working alongside proteins such as ATM and ATR. CHD4 also influences the cell cycle by interacting with cyclins and cyclin-dependent kinases which maintain proper cell cycle progression and check points.

CHD4 has been linked to various cancers and neurodevelopmental disorders. Alterations or mutations in CHD4 have associations with certain cancers like colorectal cancer where it affects cellular proliferation and migration. In neurodevelopmental disorders disrupted CHD4 function may lead to chromatin misregulation contributing to intellectual disabilities. CHD4’s interactions with proteins such as p53 in cancer and MeCP2 in neurological conditions highlight its connections in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA (PubMed : 28977666, PubMed : 32543371). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 17626165, PubMed : 28977666, PubMed : 9804427). Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination (PubMed : 25593309). Involved in neurogenesis (By similarity).
See full target information CHD4
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Publications (65)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed39907108

2025

Chromatin remodeler CHD4 establishes chromatin states required for ovarian reserve formation, maintenance and male germ cell survival.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhisa Munakata,Mengwen Hu,Yuka Kitamura,Raissa G Dani,Adam L Bynder,Amelia S Fritz,Richard M Schultz,Satoshi H Namekawa

Blood 145:422-437 PubMed39437550

2024

Conserved helical motifs in the IKZF1 disordered region mediate NuRD interaction and transcriptional repression.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Zhang,Yi-Fang Wang,Alex Montoya,Ilinca Patrascan,Nehir Nebioglu,Husayn A Pallikonda,Radina Georgieva,James W D King,Holger B Kramer,Pavel V Shliaha,David S Rueda,Matthias Merkenschlager

The EMBO journal 43:2453-2485 PubMed38719994

2024

The GATAD2B-NuRD complex drives DNA:RNA hybrid-dependent chromatin boundary formation upon DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Zhichao Liu,Kamal Ajit,Yupei Wu,Wei-Guo Zhu,Monika Gullerova

Nucleic acids research 52:6811-6829 PubMed38676947

2024

CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Chen,Ming-Feng Huang,Da-Meng Fan,Yao-Hui He,Wen-Juan Zhang,Jian-Cheng Ding,Bing-Ling Peng,Xu Pan,Ya Liu,Jun Du,Ying Li,Zhi-Ying Liu,Bing-Lan Xie,Zhi-Jian Kuang,Jia Yi,Wen Liu

Scientific reports 14:8286 PubMed38594331

2024

CHD4 acts as a prognostic factor and drives radioresistance in HPV negative HNSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Geyer,Maximilian Geyer,Ute Reuning,Sarah Klapproth,Klaus-Dietrich Wolff,Markus Nieberler

Nature communications 15:2163 PubMed38461299

2024

Mi-2β promotes immune evasion in melanoma by activating EZH2 methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Cang Li,Zhengyu Wang,Licheng Yao,Xingyu Lin,Yongping Jian,Yujia Li,Jie Zhang,Jingwei Shao,Phuc D Tran,James R Hagman,Meng Cao,Yusheng Cong,Hong-Yu Li,Colin R Goding,Zhi-Xiang Xu,Xuebin Liao,Xiao Miao,Rutao Cui

Cancer research 84:241-257 PubMed37963210

2023

The Chromatin Remodeler CHD4 Sustains Ewing Sarcoma Cell Survival by Controlling Global Chromatin Architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Joana Graca Marques,Blaz Pavlovic,Quy A Ngo,Gloria Pedot,Michaela Roemmele,Larissa Volken,Samanta Kisele,Romain Perbet,Marco Wachtel,Beat W Schäfer

Human molecular genetics 32:3361-3373 PubMed37738575

2023

Divergent phenotypes in constitutive versus conditional mutant mouse models of Sifrim-Hitz-Weiss syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Larrigan,Shrilaxmi V Joshi,Pierre Mattar

PLoS pathogens 19:e1011477 PubMed37410772

2023

Changes in SUMO-modified proteins in Epstein-Barr virus infection identifies reciprocal regulation of TRIM24/28/33 complexes and the lytic switch BZLF1.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos F De La Cruz-Herrera,Michael H Tatham,Umama Z Siddiqi,Kathy Shire,Edyta Marcon,Jack F Greenblatt,Ronald T Hay,Lori Frappier

Nature communications 14:3848 PubMed37385984

2023

Dissecting the roles of MBD2 isoforms and domains in regulating NuRD complex function during cellular differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Schmolka,Ino D Karemaker,Richard Cardoso da Silva,Davide C Recchia,Vincent Spegg,Jahnavi Bhaskaran,Michael Teske,Nathalie P de Wagenaar,Matthias Altmeyer,Tuncay Baubec
View all publications
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Product promise

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