AB70469

Anti-CHD4 antibody [3F2/4]

Name: Anti-CHD4 antibody [3F2/4] Mouse monoclonal (ab70469) | Abcam
Brand: Abcam Limited
SKU: AB70469
Price: 210 USD
Availability: InStock
Rating: 5 (8 reviews)

BOND RX™ Validated
What is this?

(8 Reviews)

(54 Publications)

Mouse Monoclonal CHD4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 54 publications.

View Alternative Names

Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, CHD4

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD4 antibody [3F2/4] (AB70469)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD4 antibody [3F2/4] (AB70469)

IHC image of CHD4 staining in human breast carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70469, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3F2/4

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHD4 also known as CDH4 is a protein that functions as part of the nucleosome remodeling and deacetylase (NuRD) complex. CHD4 has a molecular mass of approximately 218 kDa. The protein contains a chromo domain a helicase domain and two PHD-type zinc fingers. It is expressed ubiquitously in human tissues with higher expression levels seen in the spleen and fetal brain. This protein plays an essential role in DNA transcription regulation by modifying chromatin structure.

Biological function summary

CHD4 acts within the NuRD complex to mediate transcriptional repression. This complex combines chromatin remodeling with histone deacetylation to alter chromatin dynamics affecting gene expression. CHD4's involvement in the complex facilitates the static organization of chromatin therefore contributing to transcriptional silencing of specific gene sets. The protein's activity is often in response to environmental and developmental cues playing an integral role in cellular differentiation and proliferation processes.

Pathways

CHD4 connects closely with DNA damage repair and cell cycle regulation pathways. It participates actively in the DNA damage response pathway by facilitating the repair of double-strand breaks working alongside proteins such as ATM and ATR. CHD4 also influences the cell cycle by interacting with cyclins and cyclin-dependent kinases which maintain proper cell cycle progression and check points.

CHD4 has been linked to various cancers and neurodevelopmental disorders. Alterations or mutations in CHD4 have associations with certain cancers like colorectal cancer where it affects cellular proliferation and migration. In neurodevelopmental disorders disrupted CHD4 function may lead to chromatin misregulation contributing to intellectual disabilities. CHD4’s interactions with proteins such as p53 in cancer and MeCP2 in neurological conditions highlight its connections in disease mechanisms.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

ATP-dependent helicase that binds and distorts nucleosomal DNA (PubMed : 28977666, PubMed : 32543371). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 17626165, PubMed : 28977666, PubMed : 9804427). Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination (PubMed : 25593309). Involved in neurogenesis (By similarity).

See full target information CHD4

style

left-column

Publications (54)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 19:e1011477 PubMed37410772

2023

Changes in SUMO-modified proteins in Epstein-Barr virus infection identifies reciprocal regulation of TRIM24/28/33 complexes and the lytic switch BZLF1.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos F De La Cruz-Herrera,Michael H Tatham,Umama Z Siddiqi,Kathy Shire,Edyta Marcon,Jack F Greenblatt,Ronald T Hay,Lori Frappier

Nature communications 14:3848 PubMed37385984

2023

Dissecting the roles of MBD2 isoforms and domains in regulating NuRD complex function during cellular differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Schmolka,Ino D Karemaker,Richard Cardoso da Silva,Davide C Recchia,Vincent Spegg,Jahnavi Bhaskaran,Michael Teske,Nathalie P de Wagenaar,Matthias Altmeyer,Tuncay Baubec

International journal of molecular sciences 24: PubMed37445725

2023

Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4 Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1).

Applications

Unspecified application

Species

Unspecified reactive species

Ilyas Chohra,Subhajit Giri,Brigitte Malgrange

iScience 26:106701 PubMed37207277

2023

Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Han H Hsu,Greta Pintacuda,Ruize Liu,Eugeniu Nacu,April Kim,Kalliopi Tsafou,Natalie Petrossian,William Crotty,Jung Min Suh,Jackson Riseman,Jacqueline M Martin,Julia C Biagini,Daya Mena,Joshua K T Ching,Edyta Malolepsza,Taibo Li,Tarjinder Singh,Tian Ge,Shawn B Egri,Benjamin Tanenbaum,Caroline R Stanclift,Annie M Apffel,Steven A Carr,Monica Schenone,Jake Jaffe,Nadine Fornelos,Hailiang Huang,Kevin C Eggan,Kasper Lage

Cell genomics 3:100250 PubMed36950384

2023

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Greta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper Lage

International journal of molecular sciences 23: PubMed36076959

2022

Interplay between the Chd4/NuRD Complex and the Transcription Factor Znf219 Controls Cardiac Cell Identity.

Applications

Unspecified application

Species

Unspecified reactive species

Fadoua El Abdellaoui-Soussi,Paula S Yunes-Leites,Dolores López-Maderuelo,Fernando García-Marqués,Jesús Vázquez,Juan Miguel Redondo,Pablo Gómez-Del Arco

Journal of molecular endocrinology 69:329-341 PubMed35521759

2022

The Chd4 subunit of the NuRD complex regulates Pdx1-controlled genes involved in β-cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca K Davidson,Staci A Weaver,Nolan Casey,Sukrati Kanojia,Elise Hogarth,Rebecca Schneider Aguirre,Emily K Sims,Carmella Evans-Molina,Jason M Spaeth

Cell reports 38:110534 PubMed35320709

2022

The mTOR chromatin-bound interactome in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine R Dufour,Charlotte Scholtes,Ming Yan,Yonghong Chen,Lingwei Han,Ting Li,Hui Xia,Qiyun Deng,Mathieu Vernier,Vincent Giguère

Cell reports 38:110524 PubMed35294876

2022

The BTB transcription factors ZBTB11 and ZFP131 maintain pluripotency by repressing pro-differentiation genes.

Applications

Unspecified application

Species

Unspecified reactive species

Görkem Garipler,Congyi Lu,Alexis Morrissey,Lorena S Lopez-Zepeda,Yingzhen Pei,Simon E Vidal,Ana Paula Zen Petisco Fiore,Begüm Aydin,Matthias Stadtfeld,Uwe Ohler,Shaun Mahony,Neville E Sanjana,Esteban O Mazzoni

Nucleic acids research 50:3169-3189 PubMed35150568

2022

The DNA dioxygenase Tet1 regulates H3K27 modification and embryonic stem cell biology independent of its catalytic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Chrysanthou,Qin Tang,Joun Lee,Samuel J Taylor,Yilin Zhao,Ulrich Steidl,Deyou Zheng,Meelad M Dawlaty

View all publications

style

left-column

style

left-column

style

right-column