AB72418

Anti-CHD4 antibody

Name: Anti-CHD4 antibody (ab72418) | Abcam
Brand: Abcam Limited
SKU: AB72418
Price: 480 USD
Availability: InStock

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(50 Publications)

Rabbit Polyclonal CHD4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 50 publications. Immunogen corresponding to Synthetic Peptide within Human CHD4.

View Alternative Names

Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, CHD4

2 Images

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Immunoprecipitation - Anti-CHD4 antibody (AB72418)

Detection of CHD4 by Western Blot of Immunprecipitate.
ab72418 at 0.1µg/ml staining CHD4 in HeLa whole cell lysate immunoprecipitated using ab72418 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-CHD4 antibody (ab72418)

Predicted band size: 218 kDa

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Supplier Data

Western blot - Anti-CHD4 antibody (AB72418)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-CHD4 antibody (ab72418) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 218 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CHD4. The exact immunogen used to generate this antibody is proprietary information.

Q14839

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab72418 was affinity purified using an epitope specific to CHD4 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHD4 also known as CDH4 is a protein that functions as part of the nucleosome remodeling and deacetylase (NuRD) complex. CHD4 has a molecular mass of approximately 218 kDa. The protein contains a chromo domain a helicase domain and two PHD-type zinc fingers. It is expressed ubiquitously in human tissues with higher expression levels seen in the spleen and fetal brain. This protein plays an essential role in DNA transcription regulation by modifying chromatin structure.

Biological function summary

CHD4 acts within the NuRD complex to mediate transcriptional repression. This complex combines chromatin remodeling with histone deacetylation to alter chromatin dynamics affecting gene expression. CHD4's involvement in the complex facilitates the static organization of chromatin therefore contributing to transcriptional silencing of specific gene sets. The protein's activity is often in response to environmental and developmental cues playing an integral role in cellular differentiation and proliferation processes.

Pathways

CHD4 connects closely with DNA damage repair and cell cycle regulation pathways. It participates actively in the DNA damage response pathway by facilitating the repair of double-strand breaks working alongside proteins such as ATM and ATR. CHD4 also influences the cell cycle by interacting with cyclins and cyclin-dependent kinases which maintain proper cell cycle progression and check points.

CHD4 has been linked to various cancers and neurodevelopmental disorders. Alterations or mutations in CHD4 have associations with certain cancers like colorectal cancer where it affects cellular proliferation and migration. In neurodevelopmental disorders disrupted CHD4 function may lead to chromatin misregulation contributing to intellectual disabilities. CHD4’s interactions with proteins such as p53 in cancer and MeCP2 in neurological conditions highlight its connections in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent helicase that binds and distorts nucleosomal DNA (PubMed : 28977666, PubMed : 32543371). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 17626165, PubMed : 28977666, PubMed : 9804427). Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination (PubMed : 25593309). Involved in neurogenesis (By similarity).

See full target information CHD4

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Publications (50)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6314 PubMed37813869

2023

Regulation of CTCF loop formation during pancreatic cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Lyu,M Jordan Rowley,Michael J Kulik,Stephen Dalton,Victor G Corces

American journal of human genetics 110:442-459 PubMed36812914

2023

Epigenetic regulation of plastin 3 expression by the macrosatellite DXZ4 and the transcriptional regulator CHD4.

Applications

Unspecified application

Species

Unspecified reactive species

Eike A Strathmann,Irmgard Hölker,Nikolai Tschernoster,Seyyedmohsen Hosseinibarkooie,Julien Come,Cecile Martinat,Janine Altmüller,Brunhilde Wirth

International journal of molecular sciences 23: PubMed36076959

2022

Interplay between the Chd4/NuRD Complex and the Transcription Factor Znf219 Controls Cardiac Cell Identity.

Applications

Unspecified application

Species

Unspecified reactive species

Fadoua El Abdellaoui-Soussi,Paula S Yunes-Leites,Dolores López-Maderuelo,Fernando García-Marqués,Jesús Vázquez,Juan Miguel Redondo,Pablo Gómez-Del Arco

Journal of molecular endocrinology 69:329-341 PubMed35521759

2022

The Chd4 subunit of the NuRD complex regulates Pdx1-controlled genes involved in β-cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca K Davidson,Staci A Weaver,Nolan Casey,Sukrati Kanojia,Elise Hogarth,Rebecca Schneider Aguirre,Emily K Sims,Carmella Evans-Molina,Jason M Spaeth

Epigenetics & chromatin 15:16 PubMed35568926

2022

Mouse Chd4-NURD is required for neonatal spermatogonia survival and normal gonad development.

Applications

Unspecified application

Species

Unspecified reactive species

Rodrigo O de Castro,Agustin Carbajal,Luciana Previato de Almeida,Victor Goitea,Courtney T Griffin,Roberto J Pezza

Frontiers in pediatrics 10:881287 PubMed35615634

2022

Identification of SOX2 Interacting Proteins in the Developing Mouse Lung With Potential Implications for Congenital Diaphragmatic Hernia.

Applications

Unspecified application

Species

Unspecified reactive species

Kim A A Schilders,Gabriëla G Edel,Evelien Eenjes,Bianca Oresta,Judith Birkhoff,Anne Boerema-de Munck,Marjon Buscop-van Kempen,Panagiotis Liakopoulos,Petros Kolovos,Jeroen A A Demmers,Raymond Poot,Rene M H Wijnen,Dick Tibboel,Robbert J Rottier

Genes & development 36:468-482 PubMed35450884

2022

CHD4 is recruited by GATA4 and NKX2-5 to repress noncardiac gene programs in the developing heart.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary L Robbe,Wei Shi,Lauren K Wasson,Angel P Scialdone,Caralynn M Wilczewski,Xinlei Sheng,Austin J Hepperla,Brynn N Akerberg,William T Pu,Ileana M Cristea,Ian J Davis,Frank L Conlon

Journal of experimental & clinical cancer research : CR 41:34 PubMed35073946

2022

SMARCA5 interacts with NUP98-NSD1 oncofusion protein and sustains hematopoietic cells transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Zivojin Jevtic,Vittoria Matafora,Francesca Casagrande,Fabio Santoro,Saverio Minucci,Massimilliano Garre',Milad Rasouli,Olaf Heidenreich,Giovanna Musco,Jürg Schwaller,Angela Bachi

Nature immunology 23:122-134 PubMed34937932

2021

Hierarchical regulation of the resting and activated T cell epigenome by major transcription factor families.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhong,Sarah K Walker,Yuri Pritykin,Christina S Leslie,Alexander Y Rudensky,Joris van der Veeken

Nature communications 12:1718 PubMed33741961

2021

Citrullination of pyruvate kinase M2 by PADI1 and PADI3 regulates glycolysis and cancer cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Sébastien Coassolo,Guillaume Davidson,Luc Negroni,Giovanni Gambi,Sylvain Daujat,Christophe Romier,Irwin Davidson

View all publications

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