AB181370

Anti-CHD4 antibody [EPR12229]

20ul selling size
RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal CHD4 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ATP-dependent chromatin remodeler CHD4, Chromo domain-containing protein 4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, CHD-4, CHD4

3 Images

Flow Cytometry (Intracellular) - Anti-CHD4 antibody [EPR12229] (AB181370)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CHD4 antibody [EPR12229] (AB181370)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling CHD4 with ab181370 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR12229] (AB181370)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR12229] (AB181370)

Immunofluorescent analysis of HeLa cells labeling CHD4 with ab181370 at 1/50 dilution.

Supplier Data

Western blot - Anti-CHD4 antibody [EPR12229] (AB181370)

All lanes:

Western blot - Anti-CHD4 antibody [EPR12229] (ab181370) at 1/10000 dilution

Lane 1:

NCCIT cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 218 kDa

false

Carrier free

Anti-CHD4 antibody [EPR12229] - BSA and Azide free
660 APC

APC Anti-CHD4 antibody [EPR12229]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CHD4 antibody [EPR12229]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CHD4 antibody [EPR12229]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CHD4 antibody [EPR12229]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CHD4 antibody [EPR12229]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CHD4 antibody [EPR12229]
578 PE

PE Anti-CHD4 antibody [EPR12229]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12229

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 95% homology with CDH3. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHD4 also known as CDH4 is a protein that functions as part of the nucleosome remodeling and deacetylase (NuRD) complex. CHD4 has a molecular mass of approximately 218 kDa. The protein contains a chromo domain a helicase domain and two PHD-type zinc fingers. It is expressed ubiquitously in human tissues with higher expression levels seen in the spleen and fetal brain. This protein plays an essential role in DNA transcription regulation by modifying chromatin structure.

Biological function summary

CHD4 acts within the NuRD complex to mediate transcriptional repression. This complex combines chromatin remodeling with histone deacetylation to alter chromatin dynamics affecting gene expression. CHD4's involvement in the complex facilitates the static organization of chromatin therefore contributing to transcriptional silencing of specific gene sets. The protein's activity is often in response to environmental and developmental cues playing an integral role in cellular differentiation and proliferation processes.

Pathways

CHD4 connects closely with DNA damage repair and cell cycle regulation pathways. It participates actively in the DNA damage response pathway by facilitating the repair of double-strand breaks working alongside proteins such as ATM and ATR. CHD4 also influences the cell cycle by interacting with cyclins and cyclin-dependent kinases which maintain proper cell cycle progression and check points.

CHD4 has been linked to various cancers and neurodevelopmental disorders. Alterations or mutations in CHD4 have associations with certain cancers like colorectal cancer where it affects cellular proliferation and migration. In neurodevelopmental disorders disrupted CHD4 function may lead to chromatin misregulation contributing to intellectual disabilities. CHD4’s interactions with proteins such as p53 in cancer and MeCP2 in neurological conditions highlight its connections in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA (PubMed : 28977666, PubMed : 32543371). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 17626165, PubMed : 28977666, PubMed : 9804427). Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination (PubMed : 25593309). Involved in neurogenesis (By similarity).

See full target information CHD4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

F1000Research 13:1061 PubMed40535172

2025

A guide to selecting high-performing antibodies for Rab10 (UniProt ID: P61026) for use in western blot, immunoprecipitation, and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Ruíz Moleón,Charles Alende,Maryam Fotouhi,Kathleen Southern,Riham Ayoubi,Carl Laflamme

Frontiers in cell and developmental biology 8:553 PubMed32714933

2020

ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4.

Applications

Unspecified application

Species

Unspecified reactive species

XiaoYun Sun,WenJun Yu,Li Li,YuHua Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CHD4 antibody [EPR12229]

Flow Cytometry (Intracellular) - Anti-CHD4 antibody [EPR12229] (AB181370)

Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR12229] (AB181370)

Western blot - Anti-CHD4 antibody [EPR12229] (AB181370)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise