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Rabbit Recombinant Monoclonal CHD4 antibody. Suitable for IP, ChIP, WB, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq and reacts with Human, Mouse samples. Cited in 1 publication.

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Images

ChIP - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (AB240640), expandable thumbnail
  • Immunoprecipitation - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (AB240640), expandable thumbnail
  • Western blot - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (AB240640), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (AB240640), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (AB240640), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPChIPWBIHC-PICC/IFFlow Cyt (Intra)ChIC/CUT&RUN-seq
Human
Tested
Tested
Tested
Not recommended
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Not recommended
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg for 25.00000 µg chromatin
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/600
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

ATP-dependent helicase that binds and distorts nucleosomal DNA (PubMed:28977666, PubMed:32543371). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:17626165, PubMed:28977666, PubMed:9804427). Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination (PubMed:25593309). Involved in neurogenesis (By similarity).

Alternative names

Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, CHD4

Recommended products

Rabbit Recombinant Monoclonal CHD4 antibody. Suitable for IP, ChIP, WB, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq and reacts with Human, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22953-38
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CHD4 also known as CDH4 is a protein that functions as part of the nucleosome remodeling and deacetylase (NuRD) complex. CHD4 has a molecular mass of approximately 218 kDa. The protein contains a chromo domain a helicase domain and two PHD-type zinc fingers. It is expressed ubiquitously in human tissues with higher expression levels seen in the spleen and fetal brain. This protein plays an essential role in DNA transcription regulation by modifying chromatin structure.

Biological function summary

CHD4 acts within the NuRD complex to mediate transcriptional repression. This complex combines chromatin remodeling with histone deacetylation to alter chromatin dynamics affecting gene expression. CHD4's involvement in the complex facilitates the static organization of chromatin therefore contributing to transcriptional silencing of specific gene sets. The protein's activity is often in response to environmental and developmental cues playing an integral role in cellular differentiation and proliferation processes.

Pathways

CHD4 connects closely with DNA damage repair and cell cycle regulation pathways. It participates actively in the DNA damage response pathway by facilitating the repair of double-strand breaks working alongside proteins such as ATM and ATR. CHD4 also influences the cell cycle by interacting with cyclins and cyclin-dependent kinases which maintain proper cell cycle progression and check points.

Associated diseases and disorders

CHD4 has been linked to various cancers and neurodevelopmental disorders. Alterations or mutations in CHD4 have associations with certain cancers like colorectal cancer where it affects cellular proliferation and migration. In neurodevelopmental disorders disrupted CHD4 function may lead to chromatin misregulation contributing to intellectual disabilities. CHD4’s interactions with proteins such as p53 in cancer and MeCP2 in neurological conditions highlight its connections in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • ChIP - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640), expandable thumbnail

    ChIP - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640)

    Chromatin was prepared from K-562 cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 μg of chromatin, 5 μg of ab240640 (red), or 5 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
    Primers and probes are from paper PMID: 23505388.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  • Immunoprecipitation - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640), expandable thumbnail

    Immunoprecipitation - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640)

    CHD4 was immunoprecipitated from 0.35 mg NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug with ab240640 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240640 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: NCCIT whole cell lysate 10ug.

    Lane 2: ab240640 IP in NCCIT whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab240640 in NCCIT whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min.

    All lanes: Immunoprecipitation - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640)

    Predicted band size: 218 kDa

  • Western blot - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640), expandable thumbnail

    Western blot - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: PMID: 28572115) .

    Exposure time: 1 second.

    All lanes: Western blot - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640) at 1/1000 dilution

    Lane 1: NCCIT (human pluripotent embryonic carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 5: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 218 kDa

    Observed band size: 280 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling CHD4 with ab240640 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining and weak cytoplasmic staining in NIH/3T3 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling CHD4 with ab240640 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining and weak cytoplasmic staining in HeLa cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling CHD4 with ab240640 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody.

  • ChIC/CUT&RUN sequencing - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 K562 (human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab240640 [EPR22953-38]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • ChIC/CUT&RUN sequencing - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 K562 (human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab240640 [EPR22953-38]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • ChIC/CUT&RUN sequencing - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-CHD4 antibody [EPR22953-38] - ChIP Grade (ab240640)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 K562 (human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab240640 [EPR22953-38]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

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