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AB259857

Anti-CHD5 antibody [EPR23858-3]

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Rabbit Recombinant Monoclonal CHD5 antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Human samples.

View Alternative Names

KIAA0444, CHD5, Chromodomain-helicase-DNA-binding protein 5, CHD-5, ATP-dependent helicase CHD5

3 Images
Flow Cytometry (Intracellular) - Anti-CHD5 antibody [EPR23858-3] (AB259857)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CHD5 antibody [EPR23858-3] (AB259857)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa(Human cervix adenocarcinoma epithelial cell, Left)/ PANC-1 (Human pancreatic epithelioid carcinoma epithelial cell, Right) cells labelling CHD5 with ab259857 at 1/500 dilution (0.1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control : HeLa (PMID : 29682202).

Immunocytochemistry/ Immunofluorescence - Anti-CHD5 antibody [EPR23858-3] (AB259857)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHD5 antibody [EPR23858-3] (AB259857)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized PANC-1 cells labelling CHD5 with ab259857 at 1/100 (5.55 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in PANC-1 cell line. Negative control : HeLa (PMID : 29682202) is observed.

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Western blot - Anti-CHD5 antibody [EPR23858-3] (AB259857)
  • WB

Lab

Western blot - Anti-CHD5 antibody [EPR23858-3] (AB259857)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID : 25247294).

Negative control : HeLa (PMID : 21931736).

Lysates were made freshly and used in WB immediately to minimize protein degradation.

Exposure time : 136 seconds

All lanes:

Western blot - Anti-CHD5 antibody [EPR23858-3] (ab259857) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 223 kDa

Observed band size: 260 kDa

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  • Carrier free

    Anti-CHD5 antibody [EPR23858-3] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23858-3

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHD5 also known as Chromodomain Helicase DNA Binding Protein 5 is a member of the chromodomain helicase DNA-binding family of proteins. CHD5 weighs around 240 kDa and plays a significant role in chromatin remodeling impacting gene transcription regulation. This protein is primarily expressed in neural tissues particularly in the brain where it influences neuronal differentiation and development. As part of a larger family CHD5 shares structural similarities with other CHD proteins involved in modifying the structure of chromatin.
Biological function summary

CHD5 acts as a tumor suppressor influencing cell cycle progression and apoptosis. It interacts with nucleosome structures as part of a larger chromatin remodeling complex. Through its activity CHD5 affects transcriptional regulation of several genes involved in neurodevelopment and carcinogenesis. It works in coordination with other chromatin remodelers such as CHD4 to maintain genomic stability by facilitating appropriate DNA packaging and gene accessibility.

Pathways

CHD5 interacts with pathways involved in cell cycle regulation and DNA damage response. It plays an important role in the p53 signaling pathway an important pathway maintaining genomic integrity and preventing tumor formation. CHD5 interacts with proteins such as p53 and HDAC1 contributing to the transcriptional regulation tasks essential for maintaining cellular homeostasis and preventing oncogenic transformations.

CHD5 has a strong association with neurodegenerative diseases and various cancers. Low expression levels of CHD5 have been linked to neuroblastoma and glioma conditions where its tumor-suppressive role is compromised. The protein’s interaction with p53 highlights its importance in activation pathways suppressed in cancerous cells. Additionally its chromatin remodeling function may hint at important insights in treating these diseases through targeted genetic or therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.
See full target information CHD5
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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

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Primary Antibodies

AB240640

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Primary Antibodies

AB172730

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com