Rabbit Recombinant Monoclonal CHD5 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-CHD5 antibody [EPR27395-47] (AB307831), expandable thumbnail](https://content.abcam.com/products/images/chd5-antibody-epr27395-47-ab307831--western-blot-img407317.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CHD5 antibody [EPR27395-47] (AB307831)")
![Western blot - Anti-CHD5 antibody [EPR27395-47] (AB307831), expandable thumbnail](https://content.abcam.com/products/images/chd5-antibody-epr27395-47-ab307831--western-blot-img407316.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CHD5 antibody [EPR27395-47] (AB307831)")
![Western blot - Anti-CHD5 antibody [EPR27395-47] (AB307831), expandable thumbnail](https://content.abcam.com/products/images/chd5-antibody-epr27395-47-ab307831--western-blot-img407315.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CHD5 antibody [EPR27395-47] (AB307831)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription (PubMed:23318260). May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3 (PubMed:23318260, PubMed:23948251). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:27806305). Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation (PubMed:23948251, PubMed:27806305). In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages (PubMed:23948251). Regulates the expression of genes involved in cell proliferation and differentiation (PubMed:17289567). Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation (PubMed:17289567). In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa (PubMed:24252660).
Alternative names
Kiaa0444, Chd5, Chromodomain-helicase-DNA-binding protein 5, CHD-5, ATP-dependent helicase CHD5
Recommended products
Rabbit Recombinant Monoclonal CHD5 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27395-47
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Pyrimidyn™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CHD5 also known as Chromodomain Helicase DNA Binding Protein 5 is a member of the chromodomain helicase DNA-binding family of proteins. CHD5 weighs around 240 kDa and plays a significant role in chromatin remodeling impacting gene transcription regulation. This protein is primarily expressed in neural tissues particularly in the brain where it influences neuronal differentiation and development. As part of a larger family CHD5 shares structural similarities with other CHD proteins involved in modifying the structure of chromatin.
- Biological function summary
CHD5 acts as a tumor suppressor influencing cell cycle progression and apoptosis. It interacts with nucleosome structures as part of a larger chromatin remodeling complex. Through its activity CHD5 affects transcriptional regulation of several genes involved in neurodevelopment and carcinogenesis. It works in coordination with other chromatin remodelers such as CHD4 to maintain genomic stability by facilitating appropriate DNA packaging and gene accessibility.
- Pathways
CHD5 interacts with pathways involved in cell cycle regulation and DNA damage response. It plays an important role in the p53 signaling pathway an important pathway maintaining genomic integrity and preventing tumor formation. CHD5 interacts with proteins such as p53 and HDAC1 contributing to the transcriptional regulation tasks essential for maintaining cellular homeostasis and preventing oncogenic transformations.
- Associated diseases and disorders
CHD5 has a strong association with neurodegenerative diseases and various cancers. Low expression levels of CHD5 have been linked to neuroblastoma and glioma conditions where its tumor-suppressive role is compromised. The protein’s interaction with p53 highlights its importance in activation pathways suppressed in cancerous cells. Additionally its chromatin remodeling function may hint at important insights in treating these diseases through targeted genetic or therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831), expandable thumbnail](https://content.abcam.com/products/images/chd5-antibody-epr27395-47-ab307831--western-blot-img407317.jpg "Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831)")
Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver, spleen (PMID:24849318).
Lysates were made freshly and used in WB immediately to minimize protein degradation.
The identity of the bands between 100 kDa and 180 kDa are unknown.
In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10,000 dilution.
Exposure time: 114 secondsAll lanes: Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Rat liver tissue lysate at 20 µg
Lane 3: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 260 kDa
Exposure time: 114s
![Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831), expandable thumbnail](https://content.abcam.com/products/images/chd5-antibody-epr27395-47-ab307831--western-blot-img407316.jpg "Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831)")
Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver, spleen (PMID:24849318).
Lysates were made freshly and used in WB immediately to minimize protein degradation.
The identity of the bands between 130 kDa and 200 kDa are unknown.
In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10,000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Mouse testis tissue lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 260 kDa
Exposure time: 180s
![Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831), expandable thumbnail](https://content.abcam.com/products/images/chd5-antibody-epr27395-47-ab307831--western-blot-img407315.jpg "Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831)")
Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: HeLa (PMID: 21931736).
Lysates were made freshly and used in WB immediately to minimize protein degradation.
The identity of the bands between 15 kDa and 100 kDa are unknown.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200,000 dilution.
Exposure time: 37 secondsAll lanes: Western blot - Anti-CHD5 antibody [EPR27395-47] (ab307831) at 1/1000 dilution
Lane 1: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 260 kDa
Exposure time: 37s
Downloads
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