Anti-CHD5 antibody [EPR27395-47] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-CHD5 antibody [EPR27395-47] - BSA and Azide free (AB307832)

This data was developed using ab307831, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : HeLa (PMID : 21931736). Lysates were made freshly and used in WB immediately to minimize protein degradation. The identity of the bands between 15 kDa and 100 kDa are unknown. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200,000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-CHD5 antibody [EPR27395-47] (<a href='/en-us/products/primary-antibodies/chd5-antibody-epr27395-47-ab307831'>ab307831</a>) at 1/1000 dilution

Lane 1:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 260 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-CHD5 antibody [EPR27395-47] - BSA and Azide free (AB307832)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver, spleen (PMID : 24849318). Lysates were made freshly and used in WB immediately to minimize protein degradation. The identity of the bands between 100 kDa and 180 kDa are unknown. In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10,000 dilution. Exposure time : 114 seconds

All lanes:

Western blot - Anti-CHD5 antibody [EPR27395-47] (<a href='/en-us/products/primary-antibodies/chd5-antibody-epr27395-47-ab307831'>ab307831</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 260 kDa

false

Exposure time: 114s

• WB

Supplier Data

Western blot - Anti-CHD5 antibody [EPR27395-47] - BSA and Azide free (AB307832)

This data was developed using ab307831, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver, spleen (PMID : 24849318). Lysates were made freshly and used in WB immediately to minimize protein degradation. The identity of the bands between 130 kDa and 200 kDa are unknown. In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10,000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-CHD5 antibody [EPR27395-47] (<a href='/en-us/products/primary-antibodies/chd5-antibody-epr27395-47-ab307831'>ab307831</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 260 kDa

false

Exposure time: 180s