JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB51330

Anti-CHD6 antibody [2174C2a]

1

(1 Review)

|

(2 Publications)

Mouse Monoclonal CHD6 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CHD6.

View Alternative Names

CHD5, KIAA1335, RIGB, CHD6, Chromodomain-helicase-DNA-binding protein 6, CHD-6, ATP-dependent helicase CHD6, Radiation-induced gene B protein

1 Images
Western blot - Anti-CHD6 antibody [2174C2a] (AB51330)
  • WB

Unknown

Western blot - Anti-CHD6 antibody [2174C2a] (AB51330)

All lanes:

Western blot - Anti-CHD6 antibody [2174C2a] (ab51330) at 1/50 dilution

All lanes:

HeLa whole cell lysate at 25 µg

Secondary

All lanes:

anti-mouse IgG at 1/2500 dilution

Predicted band size: 305 kDa

Observed band size: 305 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2174C2a

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human CHD6. The exact immunogen used to generate this antibody is proprietary information.

Q8TD26

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CHD6 protein also known as chromodomain-helicase-DNA-binding protein 6 is a member of the CHD family characterized by the presence of chromo domains and helicase domains. CHD6 has an approximate molecular mass of 290 kDa. It is widely expressed in human tissues with significant levels found in the brain heart and skeletal muscles. CHD6 plays a role in nucleosome remodeling which involves the alteration of chromatin structure to regulate DNA accessibility for transcription replication and repair.
Biological function summary

Chromodomain-helicase enzymes such as CHD6 help to reorganize chromatin structure making it critical for access and expression of genetic information. CHD6 functions within a complex system which influences diverse transcriptional programs through chromatin structure modulation. It primarily interacts with factors involved in chromatin dynamics allowing it to participate actively in numerous cellular processes essential for maintaining normal cell functions and genetic expression stability.

Pathways

The activity of CHD6 is essential in gene expression and DNA repair pathways. CHD6 is linked with the major transcription pathways such as RNA polymerase II transcription. It also frequently interacts with proteins like BRG1 another chromatin-remodeling factor to maintain precise regulation of these pathways. Together these proteins coordinate to ensure the correct transcriptional response and chromatin environment necessary for effective cell functioning.

Aberrant CHD6 function associates with a range of pathophysiological conditions including neurodevelopmental disorders and cancer. Mutations or dysregulation in the expression of CHD6 can lead to conditions like CHARGE syndrome characterized by multiple congenital anomalies. CHD6's interaction with proteins like p53 a well-known tumor suppressor highlights its involvement in pathways that maintain genomic integrity and prevent tumorigenesis. Understanding these interactions can provide insight into therapeutic targets for related diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

DNA-dependent ATPase that plays a role in chromatin remodeling. Regulates transcription by disrupting nucleosomes in a largely non-sliding manner which strongly increases the accessibility of chromatin (PubMed : 28533432). Activates transcription of specific genes in response to oxidative stress through interaction with NFE2L2.. (Microbial infection) Acts as a transcriptional repressor of different viruses including influenza virus or papillomavirus. During influenza virus infection, the viral polymerase complex localizes CHD6 to inactive chromatin where it gets degraded in a proteasome independent-manner.
See full target information CHD6
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1026 PubMed39863586

2025

CHD6 has poly(ADP-ribose)- and DNA-binding domains and regulates PARP1/2-trapping inhibitor sensitivity via abasic site repair.

Applications

Unspecified application

Species

Unspecified reactive species

Luc Provencher,Wilson Nartey,Peter M Brownlee,Austin W Atkins,Jean-Philippe Gagné,Lou Baudrier,Nicholas S Y Ting,Cortt G Piett,Shujuan Fang,Dustin D Pearson,Shaun Moore,Pierre Billon,Zachary D Nagel,Guy G Poirier,Gareth J Williams,Aaron A Goodarzi

Cellular microbiology 13:1894-906 PubMed21899694

2011

CHD6 chromatin remodeler is a negative modulator of influenza virus replication that relocates to inactive chromatin upon infection.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Alfonso,Thomas Lutz,Ariel Rodriguez,J Pablo Chavez,Paloma Rodriguez,Silvia Gutierrez,Amelia Nieto
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB114095

Anti-CHD6 antibody

Immunoprecipitation - Anti-CHD6 antibody (AB114095)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB309968

Alexa Fluor® 488 Anti-c-Kit antibody [YR145]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-c-Kit antibody [YR145] (AB309968)

  • 0
  • 0
Primary Antibodies

AB190232

Anti-MRPL13 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL13 antibody (AB190232)

  • 0
  • 0
Primary Antibodies

AB308167

Anti-Miz1 antibody [HL1548]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Miz1 antibody [HL1548] (AB308167)

  • 0
  • 0
Primary Antibodies

AB47062

Anti-TRIM33 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TRIM33 antibody (AB47062)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com