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AB114095

Anti-CHD6 antibody

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(2 Publications)

Rabbit Polyclonal CHD6 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CHD6 aa 2650 to C-terminus.

View Alternative Names

CHD5, KIAA1335, RIGB, CHD6, ATP-dependent chromatin remodeler CHD6, Chromo domain-containing protein 6, Radiation-induced gene B protein, CHD-6

2 Images
Immunoprecipitation - Anti-CHD6 antibody (AB114095)
  • IP

Unknown

Immunoprecipitation - Anti-CHD6 antibody (AB114095)

ab114095, at 1 µg/ml, staining CHD6 in HeLa whole cell lysate immunoprecipitated using ab114095 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-CHD6 antibody (ab114095)

Predicted band size: 305 kDa

false

Western blot - Anti-CHD6 antibody (AB114095)
  • WB

Unknown

Western blot - Anti-CHD6 antibody (AB114095)

All lanes:

Western blot - Anti-CHD6 antibody (ab114095) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Predicted band size: 305 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CHD6 aa 2650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TD26

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab114095 was affinity purified using an epitope specific to CHD6 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CHD6 protein also known as chromodomain-helicase-DNA-binding protein 6 is a member of the CHD family characterized by the presence of chromo domains and helicase domains. CHD6 has an approximate molecular mass of 290 kDa. It is widely expressed in human tissues with significant levels found in the brain heart and skeletal muscles. CHD6 plays a role in nucleosome remodeling which involves the alteration of chromatin structure to regulate DNA accessibility for transcription replication and repair.
Biological function summary

Chromodomain-helicase enzymes such as CHD6 help to reorganize chromatin structure making it critical for access and expression of genetic information. CHD6 functions within a complex system which influences diverse transcriptional programs through chromatin structure modulation. It primarily interacts with factors involved in chromatin dynamics allowing it to participate actively in numerous cellular processes essential for maintaining normal cell functions and genetic expression stability.

Pathways

The activity of CHD6 is essential in gene expression and DNA repair pathways. CHD6 is linked with the major transcription pathways such as RNA polymerase II transcription. It also frequently interacts with proteins like BRG1 another chromatin-remodeling factor to maintain precise regulation of these pathways. Together these proteins coordinate to ensure the correct transcriptional response and chromatin environment necessary for effective cell functioning.

Aberrant CHD6 function associates with a range of pathophysiological conditions including neurodevelopmental disorders and cancer. Mutations or dysregulation in the expression of CHD6 can lead to conditions like CHARGE syndrome characterized by multiple congenital anomalies. CHD6's interaction with proteins like p53 a well-known tumor suppressor highlights its involvement in pathways that maintain genomic integrity and prevent tumorigenesis. Understanding these interactions can provide insight into therapeutic targets for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent chromatin-remodeling factor (PubMed : 17027977, PubMed : 28533432). Regulates transcription by disrupting nucleosomes in a largely non-sliding manner which strongly increases the accessibility of chromatin; nucleosome disruption requires ATP (PubMed : 28533432). Activates transcription of specific genes in response to oxidative stress through interaction with NFE2L2.. (Microbial infection) Acts as a transcriptional repressor of different viruses including influenza virus or papillomavirus. During influenza virus infection, the viral polymerase complex localizes CHD6 to inactive chromatin where it gets degraded in a proteasome independent-manner.
See full target information CHD6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 8:130 PubMed36473865

2022

The chromatin remodeler CHD6 promotes colorectal cancer development by regulating TMEM65-mediated mitochondrial dynamics via EGF and Wnt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Boyu Zhang,Qingxin Liu,Weijie Wen,Han Gao,Wenxia Wei,Aiwen Tang,Baifu Qin,Haiwen Lyu,Xiangqi Meng,Kai Li,Huilin Jin,Fenghai Yu,Qihao Pan,Junzhong Lin,Mong-Hong Lee

International journal of molecular medicine 41:155-163 PubMed29115379

2017

Histone deacetylase 3 is associated with gastric cancer cell growth via the miR-454-mediated targeting of CHD5.

Applications

Unspecified application

Species

Unspecified reactive species

Guangru Xu,Hongxing Zhu,Minghui Zhang,Jinhua Xu
View all publications
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Product promise

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