Rabbit Polyclonal CHD6 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CHD6 aa 2650 to C-terminus.
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Publications
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- Cell discovery 8:1302022The chromatin remodeler CHD6 promotes colorectal cancer development by regulating TMEM65-mediated mitochondrial dynamics via EGF and Wnt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoyu Zhang,Qingxin Liu,Weijie Wen,Han Gao,Wenxia Wei,Aiwen Tang,Baifu Qin,Haiwen Lyu,Xiangqi Meng,Kai Li,Huilin Jin,Fenghai Yu,Qihao Pan,Junzhong Lin,Mong-Hong LeePubMed 36473865
- International journal of molecular medicine 41:155-1632017Histone deacetylase 3 is associated with gastric cancer cell growth via the miR-454-mediated targeting of CHD5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangru Xu,Hongxing Zhu,Minghui Zhang,Jinhua XuPubMed 29115379
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CHD6 aa 2650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TD26
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
DNA-dependent ATPase that plays a role in chromatin remodeling. Regulates transcription by disrupting nucleosomes in a largely non-sliding manner which strongly increases the accessibility of chromatin (PubMed:28533432). Activates transcription of specific genes in response to oxidative stress through interaction with NFE2L2. (Microbial infection) Acts as a transcriptional repressor of different viruses including influenza virus or papillomavirus. During influenza virus infection, the viral polymerase complex localizes CHD6 to inactive chromatin where it gets degraded in a proteasome independent-manner.
Alternative names
CHD5, KIAA1335, RIGB, CHD6, Chromodomain-helicase-DNA-binding protein 6, CHD-6, ATP-dependent helicase CHD6, Radiation-induced gene B protein
Recommended products
Rabbit Polyclonal CHD6 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CHD6 aa 2650 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CHD6 aa 2650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TD26
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab114095 was affinity purified using an epitope specific to CHD6 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CHD6 protein also known as chromodomain-helicase-DNA-binding protein 6 is a member of the CHD family characterized by the presence of chromo domains and helicase domains. CHD6 has an approximate molecular mass of 290 kDa. It is widely expressed in human tissues with significant levels found in the brain heart and skeletal muscles. CHD6 plays a role in nucleosome remodeling which involves the alteration of chromatin structure to regulate DNA accessibility for transcription replication and repair.
- Biological function summary
Chromodomain-helicase enzymes such as CHD6 help to reorganize chromatin structure making it critical for access and expression of genetic information. CHD6 functions within a complex system which influences diverse transcriptional programs through chromatin structure modulation. It primarily interacts with factors involved in chromatin dynamics allowing it to participate actively in numerous cellular processes essential for maintaining normal cell functions and genetic expression stability.
- Pathways
The activity of CHD6 is essential in gene expression and DNA repair pathways. CHD6 is linked with the major transcription pathways such as RNA polymerase II transcription. It also frequently interacts with proteins like BRG1 another chromatin-remodeling factor to maintain precise regulation of these pathways. Together these proteins coordinate to ensure the correct transcriptional response and chromatin environment necessary for effective cell functioning.
- Associated diseases and disorders
Aberrant CHD6 function associates with a range of pathophysiological conditions including neurodevelopmental disorders and cancer. Mutations or dysregulation in the expression of CHD6 can lead to conditions like CHARGE syndrome characterized by multiple congenital anomalies. CHD6's interaction with proteins like p53 a well-known tumor suppressor highlights its involvement in pathways that maintain genomic integrity and prevent tumorigenesis. Understanding these interactions can provide insight into therapeutic targets for related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CHD6 antibody (ab114095)
All lanes: Western blot - Anti-CHD6 antibody (ab114095) at 0.1 µg/mL
Lane 1: Whole cell lysate from HeLa cells at 50 µg
Lane 2: Whole cell lysate from HeLa cells at 15 µg
Lane 3: Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 305 kDa
Exposure time: 30s
Immunoprecipitation - Anti-CHD6 antibody (ab114095)
ab114095, at 1 µg/ml, staining CHD6 in HeLa whole cell lysate immunoprecipitated using ab114095 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-CHD6 antibody (ab114095)
Predicted band size: 305 kDa
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