Rabbit Recombinant Monoclonal CHD6 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-CHD6 antibody [EPR23857-29] - BSA and Azide free (AB281836), expandable thumbnail](https://content.abcam.com/products/images/chd6-antibody-epr23857-29-bsa-and-azide-free-ab281836--western-blot-img3252.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CHD6 antibody [EPR23857-29] - BSA and Azide free (AB281836)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
DNA-dependent ATPase that plays a role in chromatin remodeling. Regulates transcription by disrupting nucleosomes in a largely non-sliding manner which strongly increases the accessibility of chromatin (PubMed:28533432). Activates transcription of specific genes in response to oxidative stress through interaction with NFE2L2. (Microbial infection) Acts as a transcriptional repressor of different viruses including influenza virus or papillomavirus. During influenza virus infection, the viral polymerase complex localizes CHD6 to inactive chromatin where it gets degraded in a proteasome independent-manner.
Alternative names
CHD5, KIAA1335, RIGB, CHD6, Chromodomain-helicase-DNA-binding protein 6, CHD-6, ATP-dependent helicase CHD6, Radiation-induced gene B protein
Recommended products
Rabbit Recombinant Monoclonal CHD6 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23857-29
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab281836 is the carrier-free version of Anti-CHD6 antibody [EPR23857-29] ab259858.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CHD6 protein also known as chromodomain-helicase-DNA-binding protein 6 is a member of the CHD family characterized by the presence of chromo domains and helicase domains. CHD6 has an approximate molecular mass of 290 kDa. It is widely expressed in human tissues with significant levels found in the brain heart and skeletal muscles. CHD6 plays a role in nucleosome remodeling which involves the alteration of chromatin structure to regulate DNA accessibility for transcription replication and repair.
- Biological function summary
Chromodomain-helicase enzymes such as CHD6 help to reorganize chromatin structure making it critical for access and expression of genetic information. CHD6 functions within a complex system which influences diverse transcriptional programs through chromatin structure modulation. It primarily interacts with factors involved in chromatin dynamics allowing it to participate actively in numerous cellular processes essential for maintaining normal cell functions and genetic expression stability.
- Pathways
The activity of CHD6 is essential in gene expression and DNA repair pathways. CHD6 is linked with the major transcription pathways such as RNA polymerase II transcription. It also frequently interacts with proteins like BRG1 another chromatin-remodeling factor to maintain precise regulation of these pathways. Together these proteins coordinate to ensure the correct transcriptional response and chromatin environment necessary for effective cell functioning.
- Associated diseases and disorders
Aberrant CHD6 function associates with a range of pathophysiological conditions including neurodevelopmental disorders and cancer. Mutations or dysregulation in the expression of CHD6 can lead to conditions like CHARGE syndrome characterized by multiple congenital anomalies. CHD6's interaction with proteins like p53 a well-known tumor suppressor highlights its involvement in pathways that maintain genomic integrity and prevent tumorigenesis. Understanding these interactions can provide insight into therapeutic targets for related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-CHD6 antibody [EPR23857-29] - BSA and Azide free (ab281836), expandable thumbnail](https://content.abcam.com/products/images/chd6-antibody-epr23857-29-bsa-and-azide-free-ab281836--western-blot-img3252.jpg "Western blot - Anti-CHD6 antibody [EPR23857-29] - BSA and Azide free (ab281836)")
Western blot - Anti-CHD6 antibody [EPR23857-29] - BSA and Azide free (ab281836)
This data was developed using 259858, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were made freshly and used in WB test immediately to minimize protein degradation.
Exposure time: 158 seconds
All lanes: Western blot - Anti-CHD6 antibody [EPR23857-29] (Anti-CHD6 antibody [EPR23857-29] ab259858) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 305 kDa
Observed band size: 315 kDa
Downloads
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