Anti-CHD6 antibody [EPR26714-1]

• IHC

Supplier Data

Immunohistochemistry - Anti-CHD6 antibody [EPR26714-1] (AB302956)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling CHD6 with ab302956 at 1/50 (10.04 ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Nuclear staining on human breast (PMID : 22569290). The section was incubated with ab302956 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-CHD6 antibody [EPR26714-1] (AB302956)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CHD6 with ab302956 at 1/50 (10.04 ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Nuclear staining on human breast cancer(PMID : 22569290). The section was incubated with ab302956 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-CHD6 antibody [EPR26714-1] (AB302956)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling CHD6 with ab302956 at 1/50 (10.04 ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Nuclear staining on human cerebrum. The section was incubated with ab302956 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC

Supplier Data

Immunocytochemistry - Anti-CHD6 antibody [EPR26714-1] (AB302956)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling CHD6 with ab302956 at 1/50 (10.04 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear staining in HeLa cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CHD6 antibody [EPR26714-1] (AB302956)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling CHD6 with ab302956 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• WB

Lab

Western blot - Anti-CHD6 antibody [EPR26714-1] (AB302956)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The bands beneath the target band (315 kDa) are likely to be degraded target fragments.

All lanes:

Western blot - Anti-CHD6 antibody [EPR26714-1] (ab302956) at 1/1000 dilution

Lane 1:

Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

Hela transfected with siRNA specifically targeting CHD6, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 305 kDa

Observed band size: 315 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-CHD6 antibody [EPR26714-1] (AB302956)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The bands beneath the target band (315 kDa) are likely to be degraded target fragments.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-CHD6 antibody [EPR26714-1] (ab302956) at 1/1000 dilution

Lane 1:

Hela (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 180s