Rabbit Polyclonal CHD8 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human CHD8 aa 300-350.
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- Nucleic acids research 50:7013-70332022A dual-activity topoisomerase complex regulates mRNA translation and turnover.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuaikun Su,Yutong Xue,Alexei Sharov,Yongqing Zhang,Seung Kyu Lee,Jennifer L Martindale,Wen Li,Wai Lim Ku,Keji Zhao,Supriyo De,Weiping Shen,Payel Sen,Myriam Gorospe,Dongyi Xu,Weidong WangPubMed 35748872
- Nature communications 13:11512022Changes to gut amino acid transporters and microbiome associated with increased E/I ratio in Chd8 mouse model of ASD-like behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou Yu,Bing Zhang,Peifeng Ji,Zhenqiang Zuo,Yongxi Huang,Ning Wang,Chang Liu,Shuang-Jiang Liu,Fangqing ZhaoPubMed 35241668
- eLife 10:2021TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaochen Fan et. al.PubMed 33554859
- Cell cycle (Georgetown, Tex.) 19:3231-32482020Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Wen,Chenjiang He,Qin Zhang,Fanglin Zhang,Na Li,Yan Pan,Mengting Deng,Yue Wang,Jianping Li,Jiaxuan QiuPubMed 33198579
- Neuropathology and applied neurobiology 46:588-6012020A spontaneous missense mutation in the chromodomain helicase DNA-binding protein 8 (CHD8) gene: a novel association with congenital myasthenic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC Y Lee,M Petkova,S Morales-Gonzalez,N Gimber,J Schmoranzer,A Meisel,W Böhmerle,W Stenzel,M Schuelke,J M SchwarzPubMed 32267004
- Frontiers in molecular neuroscience 11:4812019Common CHD8 Genomic Targets Contrast With Model-Specific Transcriptional Impacts of Haploinsufficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Ayanna Wade,Kenneth Lim,Rinaldo Catta-Preta,Alex S NordPubMed 30692911
- Developmental cell 45:753-768.e82018Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuntao Zhao,Chen Dong,Magali Frah,Yaqi Deng,Corentine Marie,Feng Zhang,Lingli Xu,Zhixing Ma,Xinran Dong,Yifeng Lin,Scott Koenig,Brahim Nait-Oumesmar,Donna M Martin,Laiman N Wu,Mei Xin,Wenhao Zhou,Carlos Parras,Q Richard LuPubMed 29920279
- Nature neuroscience 20:1062-10732017Germline Chd8 haploinsufficiency alters brain development in mouse.
- Nature communications 6:64042015The autism-associated chromatin modifier CHD8 regulates other autism risk genes during human neurodevelopment.Applications:IP, ChIPReactive species:Unspecified reactive speciesJustin Cotney,Rebecca A Muhle,Stephan J Sanders,Li Liu,A Jeremy Willsey,Wei Niu,Wenzhong Liu,Lambertus Klei,Jing Lei,Jun Yin,Steven K Reilly,Andrew T Tebbenkamp,Candace Bichsel,Mihovil Pletikos,Nenad Sestan,Kathryn Roeder,Matthew W State,Bernie Devlin,James P NoonanPubMed 25752243
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99.91% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CHD8 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCK8
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted | Predicted |
| Elephant | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. Regulates alternative splicing of a core group of genes involved in neuronal differentiation, cell cycle and DNA repair. Enables H3K36me3-coupled transcription elongation and co-transcriptional RNA processing likely via interaction with HNRNPL.
Alternative names
HELSNF1, KIAA1564, CHD8, Chromodomain-helicase-DNA-binding protein 8, CHD-8, ATP-dependent helicase CHD8, Helicase with SNF2 domain 1
Recommended products
Rabbit Polyclonal CHD8 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human CHD8 aa 300-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99.91% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CHD8 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCK8
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab114126 was affinity purified using an epitope specific to CHD8 immobilized on a solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CHD8 also known as Chromodomain Helicase DNA-binding Protein 8 is a member of the ATP-dependent chromatin remodeling proteins with an approximate mass of 290 kDa. This protein plays a critical role in maintaining chromatin structure by modulating the accessibility of DNA to transcription factors and repair machinery. CHD8 is ubiquitously expressed with notable presence in the brain and other tissues. Through its ability to regulate gene expression CHD8 influences several cellular processes including embryonic development and neural function.
- Biological function summary
CHD8 acts as a chromatin remodeler involved in transcriptional regulation. It associates with multiprotein complexes such as the SWI/SNF complex to control gene expression finely. CHD8 can directly bind to promoter regions of target genes impacting their transcriptional activity. In neural development CHD8 contributes to cell differentiation axon guidance and synaptic connectivity highlighting its significance in cognitive functions.
- Pathways
CHD8 is involved in critical pathways regulating cellular proliferation and differentiation. Notably it participates in the Wnt signaling pathway and interacts with factors like β-catenin to regulate the transcription of Wnt-responsive genes. CHD8 also interacts with proteins such as CTNNB1 and TCF7L2 demonstrating its role in the modulation of signaling pathways essential for developmental processes and cellular homeostasis.
- Associated diseases and disorders
CHD8 mutations have been implicated in autism spectrum disorder (ASD) and intellectual disabilities. Aberrations in CHD8 function can result in dysregulated gene expression contributing to neurological development problems. Furthermore the protein's interactions with targets like FOXG1 and TBR1 reinforce its connection to the etiology of ASD. Understanding CHD8's role in these disorders may offer insight into potential therapeutic targets for ameliorating symptoms associated with its dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-CHD8 antibody (ab114126)
All lanes: Western blot - Anti-CHD8 antibody (ab114126) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 291 kDa
Exposure time: 30s
Immunoprecipitation - Anti-CHD8 antibody (ab114126)
ab114126 at 3ug/mg of lysate detecting CHD8 by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
Detection: chemiluminescence. Exposure time: 30 seconds.All lanes: Immunoprecipitation - Anti-CHD8 antibody (ab114126)
Predicted band size: 291 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD8 antibody (ab114126)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CHD8 with ab114126 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-CHD8 antibody (ab114126)
All lanes: Western blot - Anti-CHD8 antibody (ab114126) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: TCMK-1 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 291 kDa
Exposure time: 3min
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-CHD8 antibody (ab114126)
CHD8 western blot using anti-CHD8 antibody ab114126. Publication image and figure legend from Cotney, J., Muhle, R. A., et al., 2015, Nat Commun, PubMed 25752243.
ab114126 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab114126 please see the product overview.
Depletion of CHD8 in hNSCs significantly affects CHD8 target genes.(a) Top, Schematic depicting functional domains within CHD8. Sites in CHD8 that are targeted by knockdown shRNA constructs C and G are indicated by vertical grey bars. Bottom, Representative western blot of hNSC protein lysates demonstrating depletion of CHD8 protein levels due to transfection of each shRNA construct compared with a non-targeting transfection control (shCTL). QPCR and western blots were performed for each knockdown experiment. (b) Conserved CHD8 targets are disproportionately affected by CHD8 depletion. For each subset of CHD8 target genes shown, the P value from a Wilcoxon rank test comparing the distribution of differential expression P values in that subset versus active genes not bound by CHD8 in hNSC is plotted on the y axis, and the number of genes in the subset is plotted on the x axis (Supplementary Information). The red curve shows the smoothed (quadratic) spline fit to the data. (c) Residual values for the indicated subsets of CHD8 targets calculated from the fit lines in b. The set of CHD8 targets conserved in mouse holds the greatest fraction of genes showing differential expression by each CHD8 knockdown.
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