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AB325428

Anti-Chemerin antibody [EPR29327-18R]

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Rabbit Recombinant Monoclonal Chemerin antibody. Suitable for I-ELISA, WB and reacts with Recombinant full length protein - Human, Human samples.

View Alternative Names

TIG2, RARRES2, Retinoic acid receptor responder protein 2, Chemerin, RAR-responsive protein TIG2, Tazarotene-induced gene 2 protein

3 Images
Indirect ELISA - Anti-Chemerin antibody [EPR29327-18R] (AB325428)
  • I-ELISA

Lab

Indirect ELISA - Anti-Chemerin antibody [EPR29327-18R] (AB325428)

Indirect ELISA analysis of ab325428 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human chemerin recombinant protein.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Chemerin antibody [EPR29327-18R] (AB325428)
  • WB

Lab

Western blot - Anti-Chemerin antibody [EPR29327-18R] (AB325428)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : U-937

All lanes:

Western blot - Anti-Chemerin antibody [EPR29327-18R] (ab325428) at 1/1000 dilution

Lane 1:

Human plasma at 80 µg

Lane 2:

HepG2 culture supernatant (ultrafiltration) at 20 µg

Lane 3:

U-937 culture supernatant (ultrafiltration) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 19 kDa

false

Exposure time: 8s

Western blot - Anti-Chemerin antibody [EPR29327-18R] (AB325428)
  • WB

Lab

Western blot - Anti-Chemerin antibody [EPR29327-18R] (AB325428)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : pancreas(PMID : 17767914)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Chemerin antibody [EPR29327-18R] (ab325428) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 80 µg

Lane 2:

Human adrenal gland tissue lysate at 80 µg

Lane 3:

Human testis tissue lysate at 80 µg

Lane 4:

Human pancreas tissue lysate at 80 µg

Lane 5:

Untreated HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 80 µg

Lane 6:

HepG2 treated with 300 ng/ml BFA for 24 hours whole cell lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 19 kDa,36 kDa

false

Exposure time: 6s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29327-18R

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Acts also as a ligand for CMKLR2. Can also bind to C-C chemokine receptor-like 2 (CCRL2), but with a lower affinity than it does to CMKLR1 or CMKLR2 (PubMed : 27716822). Positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Also acts as a pro-inflammatory adipokine, causing an increase in secretion of pro-inflammatory and prodiabetic adipokines, which further impair adipose tissue metabolic function and have negative systemic effects including impaired insulin sensitivity, altered glucose and lipid metabolism, and a decrease in vascular function in other tissues. Can have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. Acts as a chemotactic factor for leukocyte populations expressing CMKLR1, particularly immature plasmacytoid dendritic cells, but also immature myeloid DCs, macrophages and natural killer cells. Exerts an anti-inflammatory role by preventing TNF/TNFA-induced VCAM1 expression and monocytes adhesion in vascular endothelial cells. The effect is mediated via inhibiting activation of NF-kappa-B and CRK/p38 through stimulation of AKT1/NOS3 signaling and nitric oxide production. Its dual role in inflammation and metabolism might provide a link between chronic inflammation and obesity, as well as obesity-related disorders such as type 2 diabetes and cardiovascular disease. Exhibits an antimicrobial function in the skin.
See full target information RARRES2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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