Rabbit Recombinant Monoclonal Chikungunya Virus antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line, Purified native protein samples.
Images
![Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (AB308396), expandable thumbnail](https://content.abcam.com/products/images/chikungunya-virus-nsp2-antibody-hl1431-ab308396--western-blot-img408169.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (AB308396)")
![Immunocytochemistry/ Immunofluorescence - Anti-Chikungunya virus nsP2 antibody [HL1431] (AB308396), expandable thumbnail](https://content.abcam.com/products/images/chikungunya-virus-nsp2-antibody-hl1431-ab308396--immunocytochemistry-immunofluorescence-img408171.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Chikungunya virus nsP2 antibody [HL1431] (AB308396)")
![Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (AB308396), expandable thumbnail](https://content.abcam.com/products/images/chikungunya-virus-nsp2-antibody-hl1431-ab308396--western-blot-img408170.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (AB308396)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Purified native protein | Not recommended | Tested |
| Transfected cell line | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein | Dilution info 1/20000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
Rabbit Recombinant Monoclonal Chikungunya Virus antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line, Purified native protein samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL1431
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396), expandable thumbnail](https://content.abcam.com/products/images/chikungunya-virus-nsp2-antibody-hl1431-ab308396--western-blot-img408169.jpg "Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396)")
Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396)
All lanes: Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396) at 1/20000 dilution
All lanes: Chikungunya Viral Lysate at 0.5 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Immunocytochemistry/ Immunofluorescence - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396), expandable thumbnail](https://content.abcam.com/products/images/chikungunya-virus-nsp2-antibody-hl1431-ab308396--immunocytochemistry-immunofluorescence-img408171.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396)")
Immunocytochemistry/ Immunofluorescence - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396)
For immunofluorescence analysis, mock and Chikungunya virus non-infected and infected cells slide were permeabilized for detection of Chikungunya virus nsP2 protein using ab308396 at 1/100 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.
![Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396), expandable thumbnail](https://content.abcam.com/products/images/chikungunya-virus-nsp2-antibody-hl1431-ab308396--western-blot-img408170.jpg "Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396)")
Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396)
Chikungunya Viral Lysate (0.5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Chikungunya virus nsP2 antibody [HL1431] (ab308396) diluted at 1/20000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Chikungunya virus nsP2 antibody [HL1431] (ab308396) at 1/20000 dilution
All lanes: Chikungunya Viral Lysate at 0.5 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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