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AB96045

Anti-CHIT1 antibody

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(1 Publication)

Rabbit Polyclonal CHIT1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Chitotriosidase-1 aa 200-450.

View Alternative Names

Chitotriosidase-1, Chitinase-1, CHIT1

1 Images
Western blot - Anti-CHIT1 antibody (AB96045)
  • WB

Unknown

Western blot - Anti-CHIT1 antibody (AB96045)

10% SDS PAGE

All lanes:

Western blot - Anti-CHIT1 antibody (ab96045) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 52 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Chitotriosidase-1 aa 200-450. The exact immunogen used to generate this antibody is proprietary information.

Q13231

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Primary Antibodies

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Primary Antibodies

AB118820

Anti-Factor H antibody [OX-24]

Trial Size

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Assay Kits

AB246541

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHIT1 or chitotriosidase is a glycosyl hydrolase enzyme with significant roles in chitin breakdown. It has an approximate mass of 50 kDa and recognizes chitin-containing substances. CHIT1 predominantly expresses in macrophages and acts as an important enzyme in innate immunity by degrading pathologically accumulated chitin. Alternative names for CHIT1 include chitinase-1 and it is distinguishable by its active role in neutralizing fungal pathogens utilizing this chitinase mechanism.
Biological function summary

CHIT1 participates in inflammatory processes and helps in tissue remodeling which is important in immune responses. CHIT1 does not typically form part of a complex but operates independently to mediate macrophage response through its chitinase activity. It handles polysaccharide elements in cellular debris impacting macrophage activation states. Macrophages utilize CHIT1 to counteract foreign bodies engulfed during phagocytosis playing essential roles in managing fungal infections.

Pathways

CHIT1 influences both the immune response and metabolic pathways through its enzyme function. One key pathway involving CHIT1 is the mannose receptor-mediated clearance pathway which clears glycoproteins from systemic circulation. CHIT1 activity mirrors that of other chitinases like AMCase although they derive from separate genetic backgrounds. CHIT1 contribution enhances macrophage functionality within phagolysosomal pathways important for broad-spectrum pathogen eradication.

CHIT1 correlates significantly with Gaucher's disease and chronic obstructive pulmonary disease (COPD). In Gaucher's disease elevated CHIT1 levels serve as a biomarker indicating macrophage activation and infiltration. In the case of COPD CHIT1 modulates inflammatory responses that contribute to disease progression and respiratory complications. CHIT1 interacts with proteins associated with immune regulation such as TNF-alpha in these diseases impacting inflammatory pathways and disease severities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.
See full target information Chitotriosidase-1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 289:4244-61 PubMed24338480

2013

The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lee G D Fryer,Bethan Jones,Emma J Duncan,Claire E Hutchison,Tozen Ozkan,Paul A Williams,Olivia Alder,Max Nieuwdorp,Anna K Townley,Arjen R Mensenkamp,David J Stephens,Geesje M Dallinga-Thie,Carol C Shoulders
View all publications
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Product promise

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