AB230610

Anti-Chitinase 3 like protein 3 antibody [EPR21248]

20ul selling size
BOND RX™ Validated
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal CHIL3 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 5 publications.

View Alternative Names

Chi3l3, Ym1, Chil3, Chitinase-like protein 3, Beta-N-acetylhexosaminidase Ym1, Chitinase-3-like protein 3, ECF-L, Eosinophil chemotactic cytokine, Secreted protein Ym1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chitinase 3 like protein 3 antibody [EPR21248] (AB230610)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chitinase 3 like protein 3 antibody [EPR21248] (AB230610)

IHC image of Chitinase 3 like protein 3 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BOND^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab230610, 0.01μg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chitinase 3 like protein 3 antibody [EPR21248] (AB230610)

IHC image of Chitinase 3 like protein 3 staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica BOND^TM system using the standard protocol F (with no post primary). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab230610, 0.01μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21248

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.01 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Product details

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/Rabbit Fc).

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy.

See full target information Chil3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 17:4238-4248 PubMed40688289

2025

Effect of pirfenidone on pulmonary fibrosis in acute lung injury via the regulation of the miR-34a-5p/TGF-β1/SMAD pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chengyue Yu,Yonghuai Li,Youhui Tu,Mengyuan Dai,Liming Fei

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2413253 PubMed40087815

2025

Citrullination of NF-κB p65 by PAD2 as a Novel Therapeutic Target for Modulating Macrophage Polarization in Acute Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yu,Yujing Song,Tao Dong,Wenlu Ouyang,Chao Quan,Liujiazi Shao,Leonard Barasa,Paul R Thompson,Mao Zhang,Jianjie Ma,Katsuo Kurabayashi,Yongqing Li

JCI insight 9: PubMed39405117

2024

Loss of PADI2 and PADI4 ameliorates sepsis-induced acute lung injury by suppressing NLRP3+ macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yu,Yujing Song,Tao Dong,Wenlu Ouyang,Liujiazi Shao,Chao Quan,Kyung Eun Lee,Tao Tan,Allan Tsung,Katsuo Kurabayashi,Hasan B Alam,Mao Zhang,Jianjie Ma,Yongqing Li

Frontiers in pharmacology 15:1460692 PubMed39386033

2024

Airspaces-derived exosomes contain disease-relevant protein signatures in a mouse model of cystic fibrosis (CF)-like mucoinflammatory lung disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Mao,Amol Suryawanshi,Sonika Patial,Yogesh Saini

Cancer science 114:4521-4534 PubMed37806311

2023

M2-like tumor-associated macrophages promote epithelial-mesenchymal transition through the transforming growth factor β/Smad/zinc finger e-box binding homeobox pathway with increased metastatic potential and tumor cell proliferation in lung squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ryota Sumitomo,Toshi Menju,Yumeta Shimazu,Toshiya Toyazaki,Naohisa Chiba,Hideaki Miyamoto,Yutaka Hirayama,Shigeto Nishikawa,Satona Tanaka,Yojiro Yutaka,Yoshito Yamada,Daisuke Nakajima,Akihiro Ohsumi,Masatsugu Hamaji,Atsuyasu Sato,Akihiko Yoshizawa,Cheng-Long Huang,Hironori Haga,Hiroshi Date

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Chitinase 3 like protein 3 antibody [EPR21248]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chitinase 3 like protein 3 antibody [EPR21248] (AB230610)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chitinase 3 like protein 3 antibody [EPR21248] (AB230610)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise