Rabbit Recombinant Monoclonal CHK1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-Chk1 antibody [E250] - BSA and Azide free (AB239821), expandable thumbnail](https://content.abcam.com/products/images/chk1-antibody-e250-bsa-and-azide-free-ab239821--immunoprecipitation-img6107.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Chk1 antibody [E250] - BSA and Azide free (AB239821)")
![Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (AB239821), expandable thumbnail](https://content.abcam.com/products/images/chk1-antibody-e250-bsa-and-azide-free-ab239821--western-blot-img16082.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (AB239821)")
![Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (AB239821), expandable thumbnail](https://content.abcam.com/products/images/chk1-antibody-e250-bsa-and-azide-free-ab239821--western-blot-img8217.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (AB239821)")
![Flow Cytometry (Intracellular) - Anti-Chk1 antibody [E250] - BSA and Azide free (AB239821), expandable thumbnail](https://content.abcam.com/products/images/chk1-antibody-e250-bsa-and-azide-free-ab239821--flow-cytometry-intracellular-img82039.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Chk1 antibody [E250] - BSA and Azide free (AB239821)")
![Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (AB239821), expandable thumbnail](https://content.abcam.com/products/images/chk1-antibody-e250-bsa-and-azide-free-ab239821--western-blot-img419441.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (AB239821)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Expected | Not recommended | Tested | Expected | Expected |
| Rat | Expected | Not recommended | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA (PubMed:11535615, PubMed:12399544, PubMed:12446774, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15650047, PubMed:15665856, PubMed:32357935). May also negatively regulate cell cycle progression during unperturbed cell cycles (PubMed:11535615, PubMed:12399544, PubMed:12446774, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15650047, PubMed:15665856). This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome (PubMed:11535615, PubMed:12399544, PubMed:12446774, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15650047, PubMed:15665856). Recognizes the substrate consensus sequence [R-X-X-S/T] (PubMed:11535615, PubMed:12399544, PubMed:12446774, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15650047, PubMed:15665856). Binds to and phosphorylates CDC25A, CDC25B and CDC25C (PubMed:12676583, PubMed:12676925, PubMed:12759351, PubMed:14559997, PubMed:14681206, PubMed:19734889, PubMed:9278511). Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C (PubMed:9278511). Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A (PubMed:12676583, PubMed:12676925, PubMed:12759351, PubMed:14681206, PubMed:19734889, PubMed:9278511). Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A (PubMed:19734889, PubMed:20090422, PubMed:9278511). Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression (PubMed:9278511). Also phosphorylates NEK6 (PubMed:18728393). Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination (PubMed:15665856). Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation (PubMed:10673501, PubMed:15659650, PubMed:16511572). Also promotes repair of DNA cross-links through phosphorylation of FANCE (PubMed:17296736). Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A (PubMed:12660173, PubMed:12955071). This may enhance chromatin assembly both in the presence or absence of DNA damage (PubMed:12660173, PubMed:12955071). May also play a role in replication fork maintenance through regulation of PCNA (PubMed:18451105). May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones (By similarity). Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes (By similarity). May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest (PubMed:17380128). Phosphorylates SPRTN, promoting SPRTN recruitment to chromatin (PubMed:31316063). Reduces replication stress and activates the G2/M checkpoint, by phosphorylating and inactivating PABIR1/FAM122A and promoting the serine/threonine-protein phosphatase 2A-mediated dephosphorylation and stabilization of WEE1 levels and activity (PubMed:33108758). Isoform 2. Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition.
Alternative names
CHK1, CHEK1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1
Recommended products
Rabbit Recombinant Monoclonal CHK1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- E250
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab239821 is the carrier-free version of Anti-Chk1 antibody [E250] ab32531.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821), expandable thumbnail](https://content.abcam.com/products/images/chk1-antibody-e250-bsa-and-azide-free-ab239821--immunoprecipitation-img6107.jpg "Immunoprecipitation - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821)")
Immunoprecipitation - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821)
This data was developed using Anti-Chk1 antibody [E250] ab32531, the same antibody clone in a different buffer formulation.
Purified Anti-Chk1 antibody [E250] ab32531 at 1/20 dilution (1μg) immunoprecipitating Chk1 in K-562 whole cell lysate.
Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10μg
Lane 2 (+): Anti-Chk1 antibody [E250] ab32531 + K-562 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Chk1 antibody [E250] ab32531 in K-562 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 54 kDaAll lanes: Immunoprecipitation - Anti-Chk1 antibody [E250] (Anti-Chk1 antibody [E250] ab32531)
Predicted band size: 54 kDa
![Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821), expandable thumbnail](https://content.abcam.com/products/images/chk1-antibody-e250-bsa-and-azide-free-ab239821--western-blot-img16082.jpg "Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821)")
Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821)
This data was developed using Anti-Chk1 antibody [E250] ab32531, the same antibody clone in a different buffer formulation.
We are unsure how to define the extra bands.
All lanes: Western blot - Anti-Chk1 antibody [E250] (Anti-Chk1 antibody [E250] ab32531) at 1/10000 dilution
Lane 1: MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysate at 15 µg
Lane 2: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
![Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821), expandable thumbnail](https://content.abcam.com/products/images/chk1-antibody-e250-bsa-and-azide-free-ab239821--western-blot-img8217.jpg "Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821)")
Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821)
All lanes: Purfiied at 1/1000 dilution
Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
![Flow Cytometry (Intracellular) - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821), expandable thumbnail](https://content.abcam.com/products/images/chk1-antibody-e250-bsa-and-azide-free-ab239821--flow-cytometry-intracellular-img82039.jpg "Flow Cytometry (Intracellular) - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821)")
Flow Cytometry (Intracellular) - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821)
This data was developed using Anti-Chk1 antibody [E250] ab32531, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Chk1 with purified Anti-Chk1 antibody [E250] ab32531 at 1/30 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821), expandable thumbnail](https://content.abcam.com/products/images/chk1-antibody-e250-bsa-and-azide-free-ab239821--western-blot-img419441.jpg "Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821)")
Western blot - Anti-Chk1 antibody [E250] - BSA and Azide free (ab239821)
This data was developed using Anti-Chk1 antibody [E250] ab32531, the same antibody clone in a different buffer formulation.
Anti-CHEK1 antibody [E250] (Anti-Chk1 antibody [E250] ab32531) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Chk1 antibody [E250] ab32531 was shown to bind specifically to CHEK1. A band was observed at 57 kDa in wild-type A549 cell lysates with a reduction in signal observed at this size in CHEK1 heterozygous knockout cell line. To generate this image, wild-type and CHEK1 heterozygous knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.All lanes: Western blot - Anti-Chk1 antibody [E250] (Anti-Chk1 antibody [E250] ab32531) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: Western blot - Human CHEK1 heterozygous knockout A549 cell line (Human CHEK1 heterozygous knockout A549 cell line ab276102)
Lane 2: CHEK1 knockout A549 cell lysate at 20 µg
Lane 3: A431 cell lysate at 20 µg
Lane 4: MOLT-4 cell lysate at 20 µg
SecondaryLanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 57 kDa
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