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AB199031

Anti-Chk2 antibody [EPR19236]

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(2 Publications)

Rabbit Recombinant Monoclonal Chk2 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

Chk2, Rad53, Chek2, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Checkpoint kinase 2

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] (AB199031)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] (AB199031)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Chk2 with ab199031 at 1/500 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101 ready to use). Nuclear staining on spermatogonia of mouse testis is observed (PMID : 12192050 11593395). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101 ready to use.

Nuclear staining on spermatogonia of mouse testis (PMID : 12192050 PMID : 11593395).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] (AB199031)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] (AB199031)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Chk2 with ab199031 at 1/500 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101 ready to use). Nuclear staining on mouse spleen is observed (PMID : 12192050 11593395). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101 ready to use.

Nuclear staining on mouse spleen (PMID : 12192050 PMID : 11593395).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Chk2 antibody [EPR19236] (AB199031)
  • IP

Lab

Immunoprecipitation - Anti-Chk2 antibody [EPR19236] (AB199031)

Chk2 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate with ab199031 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199031 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Exposure time 3 minutes.

Lane 1 : NIH/3T3 whole cell lysate 10 μg (Input).

Lane 2 : ab199031 IP in NIH/3T3 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199031 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

false

Exposure time: 3min

Western blot - Anti-Chk2 antibody [EPR19236] (AB199031)
  • WB

Lab

Western blot - Anti-Chk2 antibody [EPR19236] (AB199031)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

false

Exposure time: 3min

Western blot - Anti-Chk2 antibody [EPR19236] (AB199031)
  • WB

Lab

Western blot - Anti-Chk2 antibody [EPR19236] (AB199031)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution

Lane 1:

C2C12 (mouse myoblast cell line) whole cell lysate at 10 µg

Lane 2:

bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19236

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (By similarity). Under oxidative stress, promotes ATG7 ubiquitination by phosphorylating the E3 ubiquitin ligase TRIM32 at 'Ser-56' leading to positive regulation of the autophagosme assembly (By similarity).
See full target information Chek2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 12:1587391 PubMed40860911

2025

Melatonin ameliorates zearalenone-induced ovarian damage in mice through antioxidative effects.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Song,Zhong Guo,Lei Song,Jianxiu Ma,Zhifang Zhao,Yanqing Ma,Xiaoyue Ma,Wenjie Jiang,Wanjing Wang,Chongran Liu,Tongtong Wei,Ling Fu,Zhengli Qi,Jin Zhao

Cell discovery 10:115 PubMed39557828

2024

Sodium oligomannate disrupts the adherence of Rib bacteria to gut epithelia to block SAA-triggered Th1 inflammation in 5XFAD transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Wang,Zuoquan Xie,Jie Yuan,Enjing Jin,Wen Lian,Shuaishuai Chang,Guangqiang Sun,Zhengnan Feng,Hui Xu,Chen Du,Xinying Yang,Aihua Xia,Ji Qiu,Qingli Zhang,Feifei Lin,Jia Liu,Liang Li,Xiaoguang Du,Zhongping Xiao,Zhou Yi,Zhiyu Luo,Changrong Ge,Rui Li,Mingyue Zheng,Yi Jiang,Tao Wang,Jing Zhang,Qihao Guo,Meiyu Geng
View all publications
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Product promise

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