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Rabbit Recombinant Monoclonal Chk2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-Chk2 antibody [EPR19482-102] - BSA and Azide free (Capture) (AB244646), expandable thumbnail
  • Sandwich ELISA - Anti-Chk2 antibody [EPR19482-102] - BSA and Azide free (Capture) (AB244646), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978).

Alternative names

Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2, Hucds1, hCds1, CHEK2, CHK2, RAD53, CDS1

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Rabbit Recombinant Monoclonal Chk2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Alternative names

Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2, Hucds1, hCds1, CHEK2, CHK2, RAD53, CDS1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR19482-102

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab244646 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Chk2. The recommended pair for sandwich ELISA is:
Capture: ab244646, Human Chk2 Capture Antibody (unconjugated)
Detector: Anti-Chk2 antibody [EPR19482-184] - BSA and Azide free (Detector) ab244907, Human Chk2 Detector Antibody (unconjugated)
The reference range value is 0.23 - 15 ng/ml.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Anti-Chk2 antibody [EPR19482-102] - BSA and Azide free (Capture) (ab244646), expandable thumbnail

    Sandwich ELISA - Anti-Chk2 antibody [EPR19482-102] - BSA and Azide free (Capture) (ab244646)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (ab218271).

  • Sandwich ELISA - Anti-Chk2 antibody [EPR19482-102] - BSA and Azide free (Capture) (ab244646), expandable thumbnail

    Sandwich ELISA - Anti-Chk2 antibody [EPR19482-102] - BSA and Azide free (Capture) (ab244646)

    Sandwich ELISA of Human Chk2 (phospho T68) Antibody Pair - BSA and Azide free ab307458 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.HEK-293 + UV: HEK-293 treated with 60 J/m2 UV, whole cell lysate. Serial dilutions of lysates were analyzed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com