Rabbit Recombinant Monoclonal Chk2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-Chk2 antibody [EPR5528] (AB133505), expandable thumbnail](https://content.abcam.com/products/images/chk2-antibody-epr5528-ab133505--western-blot-img30698.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Chk2 antibody [EPR5528] (AB133505)")
![Western blot - Anti-Chk2 antibody [EPR5528] (AB133505), expandable thumbnail](https://content.abcam.com/products/images/chk2-antibody-epr5528-ab133505--western-blot-img116052.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Chk2 antibody [EPR5528] (AB133505)")
![Western blot - Anti-Chk2 antibody [EPR5528] (AB133505), expandable thumbnail](https://content.abcam.com/products/images/chk2-antibody-epr5528-ab133505--western-blot-img151373.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Chk2 antibody [EPR5528] (AB133505)")
![OI-RD Scanning - Anti-Chk2 antibody [EPR5528] (AB133505), expandable thumbnail](https://content.abcam.com/products/images/chk2-antibody-epr5528-ab133505--oi-rd-scanning-img85112.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Chk2 antibody [EPR5528] (AB133505)")
Publications
Filter by
- Scientific reports 6:274842016The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLidia Wrobel et. al.PubMed 27265872
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T] (PubMed:37943659). Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978). Under oxidative stress, promotes ATG7 ubiquitination by phosphorylating the E3 ubiquitin ligase TRIM32 at 'Ser-55' leading to positive regulation of the autophagosme assembly (PubMed:37943659). (Microbial infection) Phosphorylates herpes simplex virus 1/HHV-1 protein ICP0 and thus activates its SUMO-targeted ubiquitin ligase activity.
Alternative names
CDS1, CHK2, RAD53, CHEK2, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2, Hucds1, hCds1
Recommended products
Rabbit Recombinant Monoclonal Chk2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5528
- Purity
- Tissue culture supernatant
- Dissociation constant
- 1.02 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Chk2 antibody [EPR5528] (ab133505), expandable thumbnail](https://content.abcam.com/products/images/chk2-antibody-epr5528-ab133505--western-blot-img30698.jpg "Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)")
Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 μg)
Lanes 2, 6 and 10: Chk2 knockout HAP1 cell lysate (20 μg)
Lanes 3, 7 and 11: HeLa cell lysate (20 μg)
Lanes 4, 8 and 12: HEK293 cell lysate (20 μg)
Lanes 1, 2, 3 and 4: Green signal from target - ab133505 observed at 62 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control - Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal
ab133505 was shown to specifically react with Chk2 when Chk2 knockout samples were used. Wild-type and Chk2 knockout samples were subjected to SDS-PAGE. ab133505 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/10000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)
Predicted band size: 61 kDa
![Western blot - Anti-Chk2 antibody [EPR5528] (ab133505), expandable thumbnail](https://content.abcam.com/products/images/chk2-antibody-epr5528-ab133505--western-blot-img116052.jpg "Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)")
Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)
All lanes: Western blot - Anti-Chk2 antibody [EPR5528] (ab133505) at 1/10000 dilution
Lane 1: SH-SY5Y lysate at 10 µg
Lane 2: HeLa lysate at 10 µg
Lane 3: HT29 lysate at 10 µg
Lane 4: 293T cell lysate at 10 µg
SecondaryAll lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
![Western blot - Anti-Chk2 antibody [EPR5528] (ab133505), expandable thumbnail](https://content.abcam.com/products/images/chk2-antibody-epr5528-ab133505--western-blot-img151373.jpg "Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)")
Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)
Lanes 1: Wild-type HAP1 cell lysate (20 μg)
Lanes 2: Chk2 knockout HAP1 cell lysate (20 μg)
Lanes 3: HeLa cell lysate (20 μg)
Lanes 4: HEK293 cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab133505 observed at 64 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
This western blot image is a comparison between ab133505 and a competitor's rabbit polyclonal antibody.
All lanes: Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)
Predicted band size: 61 kDa
![OI-RD Scanning - Anti-Chk2 antibody [EPR5528] (ab133505), expandable thumbnail](https://content.abcam.com/products/images/chk2-antibody-epr5528-ab133505--oi-rd-scanning-img85112.jpg "OI-RD Scanning - Anti-Chk2 antibody [EPR5528] (ab133505)")
OI-RD Scanning - Anti-Chk2 antibody [EPR5528] (ab133505)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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