Anti-Chk2 antibody [EPR5528] - BSA and Azide free

• WB

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Western blot - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (AB248527)

This data was developed using ab133505, the same antibody clone in a different buffer formulation.

Lanes 1, 5, and 9 : Wild-type HAP1 cell lysate (20 μg)

Lanes 2, 6, and 10 : Chk2 knockout HAP1 cell lysate (20 μg)

Lanes 3, 7, and 11 : HeLa cell lysate (20 μg)

Lanes 4, 8, and 12 : HEK293 cell lysate (20 μg)

Lanes 1, 2, 3, and 4 : Green signal from target - ab133505 observed at 62 kDa

Lanes 5, 6, 7, and 8 : Red signal from loading control - ab8245 observed at 37 kDa

Lanes 9, 10, 11, and 12 : Merged (red and green) signal

ab133505 was shown to specifically react with Chk2 when Chk2 knockout samples were used. Wild-type and Chk2 knockout samples were subjected to SDS-PAGE. ab133505 and ab8245 (loading control to GAPDH) were diluted 1/10000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Chk2 antibody [EPR5528] (<a href='/en-us/products/primary-antibodies/chk2-antibody-epr5528-ab133505'>ab133505</a>)

Predicted band size: 61 kDa

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• WB

Unknown

Western blot - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (AB248527)

This data was developed using ab133505, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (ab248527) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HT29 lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

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• WB

Lab

Western blot - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (AB248527)

This data was developed using ab133505, the same antibody clone in a different buffer formulation.

Lanes 1 : Wild-type HAP1 cell lysate (20 μg)

Lanes 2 : Chk2 knockout HAP1 cell lysate (20 μg)

Lanes 3 : HeLa cell lysate (20 μg)

Lanes 4 : HEK293 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab133505 observed at 64 kDa. Red - loading control, ab8245, observed at 37 kDa.

This western blot image is a comparison between ab133505 and a competitor's rabbit polyclonal antibody.

All lanes:

Western blot - Anti-Chk2 antibody [EPR5528] (<a href='/en-us/products/primary-antibodies/chk2-antibody-epr5528-ab133505'>ab133505</a>)

Predicted band size: 61 kDa

false

• OI-RD Scanning

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OI-RD Scanning - Anti-Chk2 antibody [EPR5528] - BSA and Azide free (AB248527)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.