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AB106269

Anti-CHL1 antibody

5

(1 Review)

|

(6 Publications)

Rabbit Polyclonal CHL1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CHL1 aa 750-1050.

View Alternative Names

CALL, CHL1, Neural cell adhesion molecule L1-like protein, Close homolog of L1

1 Images
Western blot - Anti-CHL1 antibody (AB106269)
  • WB

Unknown

Western blot - Anti-CHL1 antibody (AB106269)

All lanes:

Western blot - Anti-CHL1 antibody (ab106269) at 1/1000 dilution

Lane 1:

HL60 cell lysate

Lane 2:

Jurkat cell lysate

Predicted band size: 135 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CHL1 aa 750-1050. The exact immunogen used to generate this antibody is proprietary information.

O00533

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in 200 µL of water
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target known as CHL1 also called L1CAM homolog or close homolog of L1 is a transmembrane protein involved in cell adhesion within the nervous system. It has a molecular mass of approximately 140 kDa. CHL1 is mainly expressed in the brain particularly during the development phase including neuronal cell bodies and axonal membranes. Its structural components enable it to facilitate homophilic and heterophilic interactions which are essential for neural cell communication.
Biological function summary

CHL1 plays a critical role in neural development and synapse formation. It is part of the immunoglobulin superfamily and forms complexes with other neural cell adhesion molecules (NCAMs). CHL1 is actively involved in neurite outgrowth cell migration and axonal guidance contributing to the proper formation of neuronal networks. Its expression is tightly regulated during neural development indicating its importance in ensuring correct neural patterning.

Pathways

CHL1 functions significantly in the axon guidance and synaptic plasticity pathways. These pathways are essential for neural differentiation and response to external stimuli. CHL1 interacts with proteins like integrins and cytoplasmic kinases such as focal adhesion kinase (FAK) modulating signal transduction processes that guide axonal development and remodeling. These interactions highlight CHL1's involvement in neural connectivity and adaptability.

CHL1 associates with psychiatric and neurodevelopmental disorders including schizophrenia and autism spectrum disorders. Altered CHL1 expression or function can lead to cognitive and behavioral deficits. Its interaction with proteins like neurofascin further emphasizes its role in maintaining neural network integrity which is often compromised in these disorders. Understanding CHL1’s interactions and functions can provide insights into therapeutic interventions for such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane (By similarity).
See full target information CHL1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed36446521

2022

Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Flavia Roşianu,Simeon R Mihaylov,Noreen Eder,Antonie Martiniuc,Suzanne Claxton,Helen R Flynn,Shamsinar Jalal,Marie-Charlotte Domart,Lucy Collinson,Mark Skehel,Ambrosius P Snijders,Matthias Krause,Sharon A Tooze,Sila K Ultanir

Pharmacogenomics and personalized medicine 15:377-392 PubMed35496348

2022

Identification of and as Two Novel Hub Genes in Endometriosis Using Integrated Bioinformatic Analysis and Experimental Verification.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Chen,Mengyun Yao,Tao Fang,Chaoshuang Ye,Yongjiang Du,Yang Jin,Ruijin Wu

Journal of cellular biochemistry 121:2655-2663 PubMed31736153

2019

Long noncoding RNA CHL1-AS1 promotes cell proliferation and migration by sponging miR-6076 to regulate CHL1 expression in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanmei Shi,Jinfen Zha,Manzhen Zuo,Qian Yan,Huamei Song

European journal of obstetrics, gynecology, and re 236:177-182 PubMed30943448

2019

Aberrant expression of CHL1 gene and long non-coding RNA CHL1-AS1, CHL1-AS2 in ovarian endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Wei Wu,Xue Ye,Ruiqiong Ma,Jianjun Luo,Honglan Zhu,Xiaohong Chang

Journal of sleep research 28:e12721 PubMed29961995

2018

In search of cerebrospinal fluid biomarkers of fatigue in multiple sclerosis: A proteomics study.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp O Valko,Bernd Roschitzki,Wolfgang Faigle,Jonas Grossmann,Christian Panse,Peter Biro,Micha Dambach,Donat R Spahn,Michael Weller,Roland Martin,Christian R Baumann

Oncotarget 8:15789-15801 PubMed28178655

2017

CHL1 hypermethylation as a potential biomarker of poor prognosis in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Esperanza Martín-Sánchez,Saioa Mendaza,Ane Ulazia-Garmendia,Iñaki Monreal-Santesteban,Idoia Blanco-Luquin,Alicia Córdoba,Francisco Vicente-García,Noemí Pérez-Janices,David Escors,Diego Megías,Paula López-Serra,Manel Esteller,José Juan Illarramendi,David Guerrero-Setas
View all publications
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