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Rabbit Recombinant Monoclonal CHM antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

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Images

Western blot - Anti-CHM antibody [EPR7142] - BSA and Azide free (AB248756), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CHM antibody [EPR7142] - BSA and Azide free (AB248756), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Tested
Not recommended
Tested
Not recommended
Mouse
Predicted
Not recommended
Predicted
Not recommended
Rat
Predicted
Not recommended
Predicted
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

Substrate-binding subunit of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B composed of RABGGTA and RABGGTB, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Besides, a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.

Alternative names

REP1, TCD, CHM, Rab proteins geranylgeranyltransferase component A 1, Choroideremia protein, Rab escort protein 1, TCD protein, REP-1

Recommended products

Rabbit Recombinant Monoclonal CHM antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7142
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248756 is the carrier-free version of Anti-CHM antibody [EPR7142] ab134964.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The CHM gene encodes for the Rab escort protein-1 (REP-1) which plays an essential role in the prenylation of Rab proteins a type of small GTPases involved in vesicle trafficking. The protein has a molecular mass of approximately 90 kDa. CHM is expressed in multiple tissues including the retina and nervous system. The alternate names for this protein include component of choroideremia and TCD which reflects its connection to cellular trafficking and transport processes.

Biological function summary

CHM is integral to the prenylation process which is important for anchoring Rab proteins to cellular membranes. Rab escort protein-1 acts within the Rab geranylgeranyltransferase complex facilitating the transfer of lipid groups to Rab proteins. This modification is necessary for proper Rab function affecting intracellular vesicle trafficking and membrane dynamics. CHM's biological actions are critical to cellular processes such as endocytosis exocytosis and autophagy.

Pathways

The CHM protein influences key cellular pathways such as the intracellular vesicle transport pathway and the autophagy pathway. Within these pathways CHM closely interacts with Rab proteins including Rab1 and Rab27 that are important in regulating vesicle movement within cells. The association of CHM with these pathways highlights its role in maintaining cellular transport and recycling mechanisms.

Associated diseases and disorders

CHM is strongly linked to choroideremia a genetic disorder leading to progressive vision loss. Mutations in the CHM gene disrupt the normal function of REP-1 affecting the prenylation of Rab proteins and impairing cellular processes in the retina. Additionally CHM mutations can have implications in disorders related to X-linked inheritance. Research also connects CHM with pathogenic proteins like Rab27 linked to granule release disorders highlighting the impact of its malfunction in disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-CHM antibody [EPR7142] - BSA and Azide free (ab248756), expandable thumbnail

    Western blot - Anti-CHM antibody [EPR7142] - BSA and Azide free (ab248756)

    This data was developed using Anti-CHM antibody [EPR7142] ab134964, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CHM antibody [EPR7142] (Anti-CHM antibody [EPR7142] ab134964) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: Y79 cell lysate at 10 µg

    Lane 3: A431 cell lysate at 10 µg

    Lane 4: 293T cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 73 kDa

    Observed band size: 95 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CHM antibody [EPR7142] - BSA and Azide free (ab248756), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CHM antibody [EPR7142] - BSA and Azide free (ab248756)

    This data was developed using Anti-CHM antibody [EPR7142] ab134964, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of CHM in A431 cells, using Anti-CHM antibody [EPR7142] ab134964 at a dilution of 1/250.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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