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AB249426

Anti-CHMP1a antibody [EPR11938(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal CHMP1a antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

CHMP1, KIAA0047, PCOLN3, PRSM1, CHMP1A, Charged multivesicular body protein 1a, Chromatin-modifying protein 1a, Vacuolar protein sorting-associated protein 46-1, CHMP1a, Vps46-1, hVps46-1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CHMP1a antibody [EPR11938(B)] - BSA and Azide free (AB249426)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CHMP1a antibody [EPR11938(B)] - BSA and Azide free (AB249426)

This data was developed using ab167412, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling CHMP1a with ab167412 at 1/250 dilution. DAPI nuclear staining is shown in blue.

Western blot - Anti-CHMP1a antibody [EPR11938(B)] - BSA and Azide free (AB249426)
  • WB

Unknown

Western blot - Anti-CHMP1a antibody [EPR11938(B)] - BSA and Azide free (AB249426)

This data was developed using ab167412, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CHMP1a antibody [EPR11938(B)] (<a href='/en-us/products/primary-antibodies/chmp1a-antibody-epr11938b-ab167412'>ab167412</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

LNCaP cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

RAW264.7 cell lysate at 10 µg

Lane 6:

PC12 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11938(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab249426 is the carrier-free version of ab167412.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein charged multivesicular body protein 1a (CHMP1a) is an essential component of the endosomal sorting complex required for transport (ESCRT)-III also known as chromatin-modifying protein 1A. CHMP1a has a molecular mass of approximately 22 kDa. It shows expression in various tissues with significant presence observed in the brain liver and kidneys. CHMP1a plays a role in the cellular process of membrane remodeling and vesicle trafficking.
Biological function summary

CHMP1a assists in cellular processes like cytokinesis and maintenance of chromatin structure. It is an important part of the ESCRT-III complex which is important for the final stages of cell division and intracellular sorting processes. CHMP1a contributes to the degradation of membrane proteins in lysosomes and the maintenance of proper cell function by controlling the fate of cell surface receptors.

Pathways

The ESCRT-III complex including CHMP1a is involved in the endosomal-lysosomal degradation pathway. This pathway plays a critical role in the down-regulation of cell signaling. CHMP1a associates with proteins such as VPS4 which is involved in the disassembly and recycling of the ESCRT-III complex for repeated use in successive cell cycles. CHMP1a also contributes to the proper functioning of the mitotic spindle assembly ensuring successful mitosis.

Defects or mutations in CHMP1a expression can associate with neurodegenerative diseases like frontotemporal dementia. Research shows implications in cancer with altered CHMP1a expression affecting tumor progression due to disrupted cell division and regulation. The proteins within its pathways such as TSG101 and ALIX also exhibit connections to oncogenic processes further highlighting the role of CHMP1a in pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing.
See full target information CHMP1A
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Product promise

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