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AB226304

Anti-CHMP2B antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal CHMP2B antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CHMP2B aa 150 to C-terminus.

View Alternative Names

CGI-84, CHMP2B, Charged multivesicular body protein 2b, CHMP2.5, Chromatin-modifying protein 2b, Vacuolar protein sorting-associated protein 2-2, CHMP2b, Vps2-2, hVps2-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP2B antibody (AB226304)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP2B antibody (AB226304)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CHMP2B using ab226304 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB staining. Counterstain : Hematoxylin (blue).

Western blot - Anti-CHMP2B antibody (AB226304)
  • WB

Supplier Data

Western blot - Anti-CHMP2B antibody (AB226304)

Cell lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-CHMP2B antibody (ab226304) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 24 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CHMP2B aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UQN3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab226304 was affinity purified using an epitope specific to CHMP2B immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
See full target information CHMP2B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:3231-3265 PubMed40240880

2025

Oligomerised RIPK1 is the main core component of the CD95 necrosome.

Applications

Unspecified application

Species

Unspecified reactive species

Nikita V Ivanisenko,Corinna König,Laura K Hillert-Richter,Maria A Feoktistova,Sabine Pietkiewicz,Max Richter,Diana Panayotova-Dimitrova,Thilo Kaehne,Inna N Lavrik

Frontiers in aging neuroscience 14:926904 PubMed35978952

2022

Intraneuronal sortilin aggregation relative to granulovacuolar degeneration, tau pathogenesis and sorfra plaque formation in human hippocampal formation.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Jiang,Chen Yang,Jia-Qi Ai,Qi-Lei Zhang,Xiao-Lu Cai,Tian Tu,Lily Wan,Xiao-Sheng Wang,Hui Wang,Aihua Pan,Jim Manavis,Wei-Ping Gai,Chong Che,Ewen Tu,Xiao-Ping Wang,Zhen-Yan Li,Xiao-Xin Yan
View all publications
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Product promise

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