AB96273

Anti-CHMP5 antibody

Name: Anti-CHMP5 antibody (ab96273) | Abcam
Brand: Abcam Limited
SKU: AB96273
Price: 520 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal CHMP5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CHMP5 aa 1-200.

View Alternative Names

C9orf83, SNF7DC2, CGI-34, HSPC177, PNAS-114, PNAS-2, CHMP5, Charged multivesicular body protein 5, Chromatin-modifying protein 5, SNF7 domain-containing protein 2, Vacuolar protein sorting-associated protein 60, Vps60, hVps60

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP5 antibody (AB96273)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP5 antibody (AB96273)

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ab96273 at 1/100 dilution

Immunocytochemistry/ Immunofluorescence - Anti-CHMP5 antibody (AB96273)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHMP5 antibody (AB96273)

Immunofluorescence analysis of HL-60 cells fixed in ice-cold MeOH for 5 min labelling CHMP5 protein using ab96273 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

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Western blot - Anti-CHMP5 antibody (AB96273)

12% SDS PAGE

All lanes:

Western blot - Anti-CHMP5 antibody (ab96273) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

H1299 whole cell lysate at 30 µg

Lane 4:

HeLa S3 whole cell lysate at 30 µg

Lane 5:

HepG2 whole cell lysate at 30 µg

Predicted band size: 25 kDa

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Supplier Data

Western blot - Anti-CHMP5 antibody (AB96273)

Samples were separated by 15 % SDS-PAGE.

All lanes:

Western blot - Anti-CHMP5 antibody (ab96273) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CHMP5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NZZ3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses) (PubMed : 14519844). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release (PubMed : 14519844).

See full target information CHMP5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Diagnostic pathology 19:4 PubMed38178187

2024

A risk model based on 10 ferroptosis regulators and markers established by LASSO-regularized linear Cox regression has a good prognostic value for ovarian cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Tingchuan Xiong,Yinghong Wang,Changjun Zhu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2300414 PubMed37387572

2023

Autophagosomes Defeat Ferroptosis by Decreasing Generation and Increasing Discharge of Free Fe in Skin Repair Cells to Accelerate Diabetic Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Shengnan Cui,Xi Liu,Yong Liu,Wenzhi Hu,Kui Ma,Qilin Huang,Ziqiang Chu,Lige Tian,Sheng Meng,Jianlong Su,Wenhua Zhang,Haihong Li,Xiaobing Fu,Cuiping Zhang

Cancer management and research 12:2381-2391 PubMed32280275

2020

B3GNT3, a Direct Target of miR-149-5p, Promotes Lung Cancer Development and Indicates Poor Prognosis of Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Tao Liu,Lei Xian,Wenzhou Liu,Jun Liu,Huafu Zhou

Methods in molecular biology (Clifton, N.J.) 1998:219-226 PubMed31250305

2019

Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting.

Applications

Unspecified application

Species

Unspecified reactive species

Francheska Son,Katharine Umphred-Wilson,Jae-Hyuck Shim,Stanley Adoro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CHMP5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP5 antibody (AB96273)

Immunocytochemistry/ Immunofluorescence - Anti-CHMP5 antibody (AB96273)

Western blot - Anti-CHMP5 antibody (AB96273)

Western blot - Anti-CHMP5 antibody (AB96273)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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