AB242221

Anti-CHMP7 antibody

Name: Anti-CHMP7 antibody (ab242221) | Abcam
Brand: Abcam Limited
SKU: AB242221
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal CHMP7 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CHMP7 aa 300-350.

View Alternative Names

Charged multivesicular body protein 7, Chromatin-modifying protein 7, CHMP7

2 Images

Supplier Data

Immunoprecipitation - Anti-CHMP7 antibody (AB242221)

CHMP7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242221 at 20 μl/reaction.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-CHMP7 antibody (ab242221) at 1/250 dilution

Lane 1:

ab242221 IP in HEK-293T whole cell lysate

Lane 2:

Control IgG IP in HEK-293T whole cell lysate

Predicted band size: 51 kDa

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Exposure time: 30s

Supplier Data

Western blot - Anti-CHMP7 antibody (AB242221)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-CHMP7 antibody (ab242221) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Predicted band size: 51 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CHMP7 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q8WUX9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to CHMP7 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ESCRT-III-like protein required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase (PubMed : 26040712). Together with SPAST, the ESCRT-III complex promotes NE sealing and mitotic spindle disassembly during late anaphase (PubMed : 26040712, PubMed : 28242692). Recruited to the reforming NE during anaphase by LEMD2 (PubMed : 28242692). Plays a role in the endosomal sorting pathway (PubMed : 16856878).

See full target information CHMP7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013217 PubMed40445976

2025

Temporal gating of nuclear import: How Merkel cell polyomavirus exploits the cell cycle for nuclear entry.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Wang,Adrienne N Eady,Isabel Amaya,Alina Stanczak,Chelsey C Spriggs

Communications biology 6:1211 PubMed38017066

2023

Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline E Strong,Jiajing Zhang,Martin Carrasco,Srikanya Kundu,Molly Boutin,Harshad D Vishwasrao,Jiamin Liu,Angelica Medina,Yu-Chi Chen,Kelli Wilson,Emily M Lee,Marc Ferrer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CHMP7 antibody

Immunoprecipitation - Anti-CHMP7 antibody (AB242221)

Western blot - Anti-CHMP7 antibody (AB242221)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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