AB37274

Anti-Cholecystokinin 8 antibody [27F3..0,5D10]

Name: Anti-Cholecystokinin 8 antibody [27F3..0,5D10] (ab37274) | Abcam
Brand: Abcam Limited
SKU: AB37274
Price: 400 USD
Availability: InStock

Be the first to review this product! Submit a review

(16 Publications)

Mouse Monoclonal Cholecystokinin antibody. Suitable for ELISA, IHC-FoFr and reacts with Rat samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human Cholecystokinin.

View Alternative Names

Cholecystokinin, CCK

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

27F3..0,5D10

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Rat

Applications

IHC-FoFr, ELISA

applications

Immunogen

Synthetic Peptide within Human Cholecystokinin. The exact immunogen used to generate this antibody is proprietary information.

P06307

Specificity

Reacts with CCK-8 and the other CCK analogs with identical C-terminal ends as well as Gastrin 17. No reactivity was observed with Human Heparin cofactor II.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>We recommend coating with CCK-8 and using the antibody in a competitive assay protocol. Increased reactivity with sulphated CCK-8 compared to non-sulphated CCK-8 has been observed.</p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS, 2.9% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cholecystokinin-8 (CCK-8) a well-known peptide hormone influences various physiological processes. At a molecular level CCK-8 has a mass of approximately 1150 Da and acts primarily by binding to specific receptors such as the CCK-A and CCK-B receptors. These receptors distribute broadly in tissues like the gastrointestinal tract and the brain. Its alternative name is sulfated octapeptide of Cholecystokinin but researchers often refer to it as CCK-8. The peptide's expression in these areas enables it to mediate digestive enzyme secretion satiety and neurotransmission.

Biological function summary

This active fragment of the larger cholecystokinin peptide exhibits functions beyond digestion. Cholecystokinin-8 plays a role in stimulating digestion by influencing the release of digestive enzymes from the pancreas and bile from the gallbladder. It interacts with other neuropeptides within the central nervous system affecting anxiety and feeding behavior. It does not act as part of a larger complex but participates in straightforward receptor-ligand interactions allowing it to exert its effects swiftly.

Pathways

Cholecystokinin-8 interacts significantly in the digestion-related and neurological pathways. In the gastrointestinal system CCK-8 is involved in the digestion pathway by promoting efficient nutrient absorption. Additionally it participates in the modulation of appetite in the satiety pathway linked closely with leptin and its regulatory effects on hunger. These pathways add to the multifunctionality of CCK-8 making it essential for maintaining homeostasis within the body's metabolic processes.

Cholecystokinin-8 has known associations with obesity and panic disorder. In obesity CCK-8's role in regulating satiety can be disrupted leading to overeating and weight gain. The peptide interacts with leptin in this context affecting energy balance and appetite control. In panic disorder alterations in CCK-8 levels can affect the central nervous system potentially triggering anxiety-related phenotypes similar to its interaction with serotonin receptors. These associations highlight CCK-8's potential as a target for therapeutic interventions in these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.

See full target information Cholecystokinin

style

left-column

Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Neurobiology of stress 33:100680 PubMed39502835

2024

Cholecystokinin-expressing interneurons mediated inhibitory transmission and plasticity in basolateral amygdala modulate stress-induced anxiety-like behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Fang,Xi Chen,Jufang He

Neurochemical research 49:3277-3296 PubMed39266897

2024

Neuromedin U Neurons in the Edinger-Westphal Nucleus Respond to Alcohol Without Interfering with the Urocortin 1 Response.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Medrano,Wissal Allaoui,Ra'fat Ehab Salim Haddad,Leila Makrini-Maleville,Emmanuel Valjent,Ilse Smolders,Viktória Kormos,Balázs Gaszner,Dimitri De Bundel

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2301110 PubMed37325895

2023

PDGF-BB-Dependent Neurogenesis Buffers Depressive-Like Behaviors by Inhibition of GABAergic Projection from Medial Septum to Dentate Gyrus.

Applications

Unspecified application

Species

Unspecified reactive species

Hou-Hong Li,Yang Liu,Hong-Sheng Chen,Ji Wang,Yu-Ke Li,Yang Zhao,Rui Sun,Jin-Gang He,Fang Wang,Jian-Guo Chen

Scientific reports 12:17851 PubMed36284123

2022

An efficient rAAV vector for protein expression in cortical parvalbumin expressing interneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Tkatch,Kristina Rysevaite-Kyguoliene,Ignas Sabeckis,Deimante Sabeckiene,Dainius H Pauza,Gytis Baranauskas

The European journal of neuroscience 52:3353-3374 PubMed32599671

2020

Dopamine depletion induces neuron-specific alterations of GABAergic transmission in the mouse striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Ida Luisa Boccalaro,Cornelia Schwerdel,Leonardo Cristiá-Lara,Jean-Marc Fritschy,Lena Rubi

Molecular neurodegeneration 15:3 PubMed31915042

2020

Contribution of GABAergic interneurons to amyloid-β plaque pathology in an APP knock-in mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Heather C Rice,Gabriele Marcassa,Iordana Chrysidou,Katrien Horré,Tracy L Young-Pearse,Ulrike C Müller,Takashi Saito,Takaomi C Saido,Robert Vassar,Joris de Wit,Bart De Strooper

Frontiers in psychiatry 10:513 PubMed31379628

2019

Possible Implication of the CA2 Hippocampal Circuit in Social Cognition Deficits Observed in the Neuroligin 3 Knock-Out Mouse, a Non-Syndromic Animal Model of Autism.

Applications

Unspecified application

Species

Unspecified reactive species

Brijesh Modi,Domenico Pimpinella,Antonio Pazienti,Paola Zacchi,Enrico Cherubini,Marilena Griguoli

The Journal of comparative neurology 527:2030-2046 PubMed30773633

2019

Cell type-specific distribution of GABA receptor subtypes in the mouse dorsal striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Ida Luisa Boccalaro,Leonardo Cristiá-Lara,Cornelia Schwerdel,Jean-Marc Fritschy,Lena Rubi

Neuropeptides 73:71-77 PubMed30503693

2018

Acute restraint stress induces cholecystokinin release via enteric apelin.

Applications

Unspecified application

Species

Unspecified reactive species

Mehmet Bülbül,Osman Sinen,Onur Bayramoğlu,Gökhan Akkoyunlu

Journal of neuroendocrinology 30:e12635 PubMed30044523

2018

Central apelin administration and restraint stress induce hypothalamic cholecystokinin release via the APJ receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Mehmet Bülbül,Osman Sinen,Leyla Abueid,Gökhan Akkoyunlu,Özlem Özsoy

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cholecystokinin 8 antibody [27F3..0,5D10]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise