AB27441

Anti-Cholecystokinin antibody

Name: Anti-Cholecystokinin antibody (ab27441) | Abcam
Brand: Abcam Limited
SKU: AB27441
Price: 515 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal Cholecystokinin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications.

View Alternative Names

Cholecystokinin, CCK

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cholecystokinin antibody (AB27441)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Cholecystokinin antibody (AB27441)

ICC/IF image of ab27441 stained CaCO2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27441 at 10μg/ml overnight at +4°C. The secondary antibody (green) was DyLight^® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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Western blot - Anti-Cholecystokinin antibody (AB27441)

All lanes:

Western blot - Anti-Cholecystokinin antibody (ab27441) at 1/25 dilution

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Brain cell lysate

Predicted band size: 13 kDa

Observed band size: 12 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Incubate for 10 minutes at room temperature.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cholecystokinin often referenced as CCK is a peptide hormone with a mass of approximately 8 kDa. It plays a mechanical role by being released from the I-cells in the small intestine particularly the duodenum. This release occurs in response to nutrients in the gut such as fats and proteins. Cholecystokinin acts by binding to its receptors CCK-A and CCK-B to execute its functions. In addition to the gut CCK is expressed in the central nervous system where it contributes to diverse neurological functions.

Biological function summary

Beyond its presence in the digestive system cholecystokinin is important for stimulating digestive enzymes secretion from the pancreas and bile release from the gallbladder. This peptide is also part of the central nervous system where it influences appetite control satiety and anxiety regulation. While not part of a classic protein complex CCK interacts with various receptors and enzymes to perform its roles in these physiological processes.

Pathways

Several important physiological pathways include cholecystokinin to regulate digestive processes and satiety signals. It is significant in the digestion pathway interacting chiefly with proteins such as trypsinogen and pancreatic lipase enhancing the breakdown of nutrients. In the neural pathway CCK influences neurotransmitter release including dopamine and serotonin highlighting its role in appetite suppression and mood regulation.

Cholecystokinin has been implicated in gastrointestinal and neurological conditions. It links to gallstone disease by influencing bile composition and flow which can affect the development of gallstones. Additionally CCK has connections to anxiety disorders given its impact on neurotransmitter systems within the brain. The peptide's engagement with CCK receptors makes it a target for studying these disorders as alterations in its signaling can contribute to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.

See full target information Cholecystokinin

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36810133

2023

Enteroendocrine cell lineages that differentially control feeding and gut motility.

Applications

Unspecified application

Species

Unspecified reactive species

Marito Hayashi,Judith A Kaye,Ella R Douglas,Narendra R Joshi,Fiona M Gribble,Frank Reimann,Stephen D Liberles

Frontiers in oncology 12:1098728 PubMed36733361

2023

CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Liang,Suohui Sun,Zheng Li,Tao Wu,Chunpu Zhang,Tao Xin

Poultry science 102:102273 PubMed36436379

2022

Cholecystokinin (CCK) and its receptors (CCK1R and CCK2R) in chickens: functional analysis and tissue expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yiping Wan,Qiuyang Deng,Zhichun Zhou,Yue Deng,Jiannan Zhang,Juan Li,Yajun Wang

JID innovations : skin science from molecules to population health 2:100153 PubMed36262666

2022

Cholecystokinin Receptor Antagonist Suppresses Melanoma Growth by Inducing Apoptosis of Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuko Funakoshi,Tetsuya Honda,Taisuke Ito,Yoshiki Tokura

Scientific reports 11:4933 PubMed33654150

2021

First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Calo,Ayelén M Blanco,Sara Comesaña,Marta Conde-Sieira,Sofia Morais,José L Soengas

The Journal of dermatology 47:1454-1456 PubMed32794215

2020

Secukinumab promotes engagement of cholecystokinin and its receptor in epidermal keratinocytes of psoriasis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuko Funakoshi,Toshiharu Fujiyama,Kazuki Tatsuno,Takatoshi Shimauchi,Yoshiki Tokura

Anais da Academia Brasileira de Ciencias 92:e20181165 PubMed32556047

2020

Localization and distribution of CCK-8, NPY, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836).

Applications

Unspecified application

Species

Unspecified reactive species

Carlos E Barrios,Juan JosÉ SantinÓn,Hugo A Domitrovic,SebastiÁn SÁnchez,David R HernÁndez

Journal of immunology (Baltimore, Md. : 1950) 202:2609-2615 PubMed30902899

2019

Cholecystokinin Downregulates Psoriatic Inflammation by Its Possible Self-Regulatory Effect on Epidermal Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuko Funakoshi,Kazuki Tatsuno,Takatoshi Shimauchi,Toshiharu Fujiyama,Taisuke Ito,Yoshiki Tokura

Gastroenterology 137:2052-62 PubMed19737569

2009

Foxa1 and Foxa2 control the differentiation of goblet and enteroendocrine L- and D-cells in mice.

Applications

IHC-P

Species

Mouse

Diana Z Ye,Klaus H Kaestner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cholecystokinin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cholecystokinin antibody (AB27441)

Western blot - Anti-Cholecystokinin antibody (AB27441)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise