Rabbit Recombinant Monoclonal CHTB antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP, I-ELISA and reacts with Transfected cell lysate - Vibrio cholerae O1 biovar El Tor str. N16961, Transfected cell line - Vibrio cholerae O1 biovar El Tor str. N16961, Recombinant fragment - Vibrio cholerae O1 biovar El Tor str. N16961 samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (AB317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--immunocytochemistry-immunofluorescence-img432095.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (AB317738)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (AB317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432107.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (AB317738)")
![Immunoprecipitation - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (AB317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--immunoprecipitation-img432140.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (AB317738)")
![ELISA - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (AB317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--elisa-img432161.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (AB317738)")
![Flow Cytometry (Intracellular) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (AB317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--flow-cytometry-intracellular-img432148.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (AB317738)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | I-ELISA | |
|---|---|---|---|---|---|---|
| Recombinant fragment - Vibrio cholerae O1 biovar El Tor str. N16961 | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Transfected cell line - Vibrio cholerae O1 biovar El Tor str. N16961 | Not recommended | Tested | Tested | Tested | Not recommended | Not recommended |
| Transfected cell lysate - Vibrio cholerae O1 biovar El Tor str. N16961 | Tested | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Vibrio cholerae O1 biovar El Tor str. N16961 | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Vibrio cholerae O1 biovar El Tor str. N16961, Recombinant fragment - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Vibrio cholerae O1 biovar El Tor str. N16961, Recombinant fragment - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Vibrio cholerae O1 biovar El Tor str. N16961, Recombinant fragment - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Vibrio cholerae O1 biovar El Tor str. N16961, Recombinant fragment - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info 1/30 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Vibrio cholerae O1 biovar El Tor str. N16961, Recombinant fragment - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info 1000 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Vibrio cholerae O1 biovar El Tor str. N16961, Transfected cell line - Vibrio cholerae O1 biovar El Tor str. N16961 | Dilution info - | Notes - |
Target data
Function
The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
Alternative names
toxB, VC_1456, ctxB, Cholera enterotoxin subunit B, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Choleragenoid
Recommended products
Rabbit Recombinant Monoclonal CHTB antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP, I-ELISA and reacts with Transfected cell lysate - Vibrio cholerae O1 biovar El Tor str. N16961, Transfected cell line - Vibrio cholerae O1 biovar El Tor str. N16961, Recombinant fragment - Vibrio cholerae O1 biovar El Tor str. N16961 samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27372-4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cholera enterotoxin subunit B also known as CTB is the non-toxic component of cholera toxin with a molecular weight of approximately 11.6 kDa. CTB plays an important role in the toxin's ability to bind to target cells. Structurally CTB forms a pentameric ring that tightly binds to the GM1 ganglioside receptor on the surface of intestinal epithelial cells. While the CTB itself displays non-pathogenicity it facilitates the delivery of the toxic subunit causing the internalization of the entire cholera toxin complex. CTB is mainly expressed by the bacterium Vibrio cholerae which is found in aquatic environments especially in regions with poor sanitation.
- Biological function summary
Cholera enterotoxin subunit B serves as a binding agent in the cholera toxin complex. Once CTB binds to the GM1 ganglioside receptors on intestinal epithelial cells it assists the A subunit also part of the cholera toxin complex in gaining entry into cells. Upon entry the A subunit triggers a cascade of events that results in dysregulation of cAMP levels leading to massive chloride ion secretion into the intestinal lumen. As a part of the entire cholera toxin CTB indirectly contributes to the excessive watery diarrhea that characterizes cholera infection.
- Pathways
Cholera enterotoxin subunit B interacts with signal transduction processes related to cyclic AMP (cAMP) pathways. After CTB-mediated internalization the A subunit activates adenylate cyclase leading to increased cAMP in intestinal epithelial cells. This activation is an important event in the signaling pathway that causes altered ion transport across the cell membrane. This pathway is linked to the CFTR protein a chloride channel regulated by cAMP which plays a critical role in maintaining ion balance in epithelial tissues. This pathway emphasizes the connection between CTB and ion transport dysregulation in cholera.
- Associated diseases and disorders
Cholera enterotoxin subunit B is associated with cholera an acute diarrheal illness caused by Vibrio cholerae. CTB's role in facilitating the cholera toxin's effects links it indirectly to dehydration and electrolyte imbalance the main clinical manifestations of the disease. While CTB itself shows potential in therapeutic applications due to its non-toxic nature in context of Vibrio cholerae infection it remains an important player in the disease's pathogenesis. Interactions between CTB GM1 ganglioside and the CFTR protein underline its relevance in the disorder's mechanism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--immunocytochemistry-immunofluorescence-img432095.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)")
Immunocytochemistry/ Immunofluorescence - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Cholera enterotoxin subunit B with ab317738 at 1/2000 (0.265 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a Cholera enterotoxin subunit B expression vector (shown in magenta) containing a EGFP-tag® (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) at 1/1000 2 ug/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432107.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag. (B) HEK-293T transfected with empty vector containing a EGFP tag. tissue labeling Cholera enterotoxin subunit B with ab317738 at 1/2000 (0.265 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, negative staining on (B) HEK-293T transfected with empty vector containing a EGFP tag. The section was incubated with ab317738 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunoprecipitation - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--immunoprecipitation-img432140.jpg "Immunoprecipitation - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)")
Immunoprecipitation - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)
Cholera enterotoxin subunit B was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate with ab317738 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317738 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate
Lane 2: ab317738 IP in 293T (human embryonic kidney epithelial cell) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317738 in 293T cells transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysateAll lanes: Immunoprecipitation - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738) at 1/30 dilution
All lanes: 293T (human embryonic kidney epithelial cell) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate at 10 µg with NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s
![ELISA - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--elisa-img432161.jpg "ELISA - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)")
ELISA - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)
Indirect ELISA analysis of ab317738 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Cholera enterotoxin subunit B.
Antigen concentration: 1000 ng/ml
The recombinant protein of Cholera enterotoxin subunit B was made in house.
![Flow Cytometry (Intracellular) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--flow-cytometry-intracellular-img432148.jpg "Flow Cytometry (Intracellular) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)")
Flow Cytometry (Intracellular) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a Cholera enterotoxin subunit B expression vector containing an EGFP tag (Middle) / 293T cells transfected with an empty expression vector containing an EGFP tag (Right) cells labelling Cholera enterotoxin subunit B with ab317738 at 1/5000 dilution (0.01 ug)/Middle and Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
![Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--western-blot-img432056.jpg "Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)")
Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)
In Western blot, Anti-GFP antibody [E385] (Anti-GFP antibody [E385] ab32146) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738) at 1/5000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a EGFP tag, whole cell lysate at 2 µg with NFDM/TBST
Lane 2: 293T transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate 2 at 2 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 40 kDa
Exposure time: 180s
![Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738), expandable thumbnail](https://content.abcam.com/products/images/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738--western-blot-img432056.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738)")
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