AB137349

Anti-Choline Acetyltransferase antibody

Name: Anti-Choline Acetyltransferase antibody (ab137349) | Abcam
Brand: Abcam Limited
SKU: AB137349
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal Choline Acetyltransferase antibody. Suitable for WB, ICC/IF, IHC-P, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CHAT aa 100-400.

View Alternative Names

Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT

6 Images

Immunohistochemistry (Frozen sections) - Anti-Choline Acetyltransferase antibody (AB137349)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Choline Acetyltransferase antibody (AB137349)

Immunohistochemical analysis of frozen mouse spinal cord tissue labeling Choline Acetyltransferase protein with ab137349 at 1/300 dilution (Red). Hoechst 33342 was used for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody (AB137349)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody (AB137349)

Immunohistochemical analysis of paraffin-embedded mouse eye tissue stained for Choline Acetyltransferase protein at cytoplasm and nucleus with ab137349 at a 1/615 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (AB137349)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (AB137349)

Immunofluorescence analysis of DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min labelling Choline Acetyltransferase protein using ab137349 at a 1/500 dilution (Green). beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Choline Acetyltransferase antibody (AB137349)

Immunohistochemical analysis of frozen adult mouse retina tissue labeling Choline Acetyltransferase protein in the amacrine cells with ab137349 at 1/250 dilution (Green). DAPI was used for nuclear staining. Red : Protein kinase C alpha staining

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody (AB137349)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody (ab137349) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extract at 30 µg

Lane 2:

Transfected 293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 82 kDa

false

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody (AB137349)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody (ab137349) at 1/500 dilution

All lanes:

IMR32 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 82 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, IHC-Fr, WB

applications

Immunogen

Recombinant Fragment Protein within Human CHAT aa 100-400. The exact immunogen used to generate this antibody is proprietary information.

P28329

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.

Biological function summary

The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.

Pathways

Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.

Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

See full target information CHAT

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37508478

2023

CXCR2 Is Deregulated in ALS Spinal Cord and Its Activation Triggers Apoptosis in Motor Neuron-Like Cells Overexpressing hSOD1-G93A.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina La Cognata,Agata Grazia D'Amico,Grazia Maugeri,Giovanna Morello,Maria Guarnaccia,Benedetta Magrì,Eleonora Aronica,Velia D'Agata,Sebastiano Cavallaro

Bratislavske lekarske listy 124:267-272 PubMed36598319

2023

Enhanced yield of cholinergic neurons from induced pluripotent stem cells (iPSC): A two-step induction protocol.

Applications

Unspecified application

Species

Unspecified reactive species

Seyed Ehsan Enderami,Maryam Nazm Bojnordi Hatef,Marzieh Sharifi Pasandi

iScience 25:104917 PubMed36051182

2022

Deciphering the dynamic niches and regeneration-associated transcriptional program of motoneurons following peripheral nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Lian Xu,Xiaodi Li,Zhifeng Chen,Jing Chen,Tao Zhang,Xiaosong Gu,Jian Yang

Journal of molecular neuroscience : MN 65:351-358 PubMed30003430

2018

Transplantation of Human-Induced Pluripotent Stem Cell-Derived Neural Precursors into Early-Stage Zebrafish Embryos.

Applications

Unspecified application

Species

Unspecified reactive species

J Strnadel,H Wang,C Carromeu,A Miyanohara,K Fujimura,E Blahovcova,V Nosal,H Skovierova,R Klemke,E Halasova

eLife 4:e08352 PubMed26052670

2015

Forebrain deletion of the dystonia protein torsinA causes dystonic-like movements and loss of striatal cholinergic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel S Pappas,Katherine Darr,Sandra M Holley,Carlos Cepeda,Omar S Mabrouk,Jenny-Marie T Wong,Tessa M LeWitt,Reema Paudel,Henry Houlden,Robert T Kennedy,Michael S Levine,William T Dauer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Choline Acetyltransferase antibody

Immunohistochemistry (Frozen sections) - Anti-Choline Acetyltransferase antibody (AB137349)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody (AB137349)

Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (AB137349)

Immunohistochemistry (Frozen sections) - Anti-Choline Acetyltransferase antibody (AB137349)

Western blot - Anti-Choline Acetyltransferase antibody (AB137349)

Western blot - Anti-Choline Acetyltransferase antibody (AB137349)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise